EMAGE mouse gene expression spatial database

EMAGE:21464

Col15a1 collagen, type XV, alpha 1 ( MGI:88449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

single cell

strong expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T396
Entity Detected:	Col15a1, collagen, type XV, alpha 1 ( MGI:88449)
Sequence:	sense strand is shown >T396 CTATGAGAGGCCTGTTCTGCACCTGGTTGCTCTGAACACACCGGTGGCTGGGGACATCCGAGCTGATTTC CAGTGCTTCCAGCAGGCCAGGGCTGCAGGCCTCCTGTCCACTTTCCGAGCCTTTCTGTCTTCACACCTGC AGGATCTCTCCACAGTCGTGCGGAAGGCAGAGAGGTTCGGCCTTCCAATTGTGAATCTCAAGGGCCAAGT GCTTTTTAACAATTGGGACTCGATATTTTCTGGTGATGGAGGTCAATTCAATACACACATTCCAATCTAC TCCTTTGACGGTCGGGATGTGATGACTGATCCTTCCTGGCCCCAGAAGGTGGTCTGGCACGGCTCCAACC CCCATGGTGTCCGTCTTGTGGACAAGTACTGTGAAGCCTGGCGAACCACGGACATGGCAGTAACCGGATT TGCCTCCCCACTGAGCACAGGGAAGATTCTGGACCAGAAAGCTTACAGCTGTGCTAACAGGC
Notes:	The probe template was PCR amplified from IMAGE:3156838 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3156838 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000902 same experiment
	EMAGE:21460 same embryo
	EMAGE:21459 same embryo
	EMAGE:21463 same embryo
	EMAGE:21461 same embryo
	EMAGE:21462 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source