EMAGE mouse gene expression spatial database

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EMAGE:21480

Gap43 growth associated protein 43 ( MGI:95639)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001126_01	euxassay_001126_02	euxassay_001126_03	euxassay_001126_04	euxassay_001126_05

euxassay_001126_06	euxassay_001126_07	euxassay_001126_08	euxassay_001126_09	euxassay_001126_10

euxassay_001126_11	euxassay_001126_12	euxassay_001126_13	euxassay_001126_14	euxassay_001126_15

euxassay_001126_16	euxassay_001126_17	euxassay_001126_18	euxassay_001126_19	euxassay_001126_20

euxassay_001126_21	euxassay_001126_22	euxassay_001126_23	euxassay_001126_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21480_wholemount_strong.wlz

21480_wholemount_moderate.wlz

21480_wholemount_weak.wlz

21480_wholemount_possible.wlz

21480_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21480_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 4 5 6 7 9 0 1

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 7 8 9

trigeminal v ganglion

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 7 8 9 0 1 2

vagus x ganglion

moderate

regional

moderate expression: see section 8 9 7

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 6 8 weak expression: see section 9

cervico-thoracic ganglion

weak

regional

weak expression: see section 0 1 6

cervical ganglion

weak

regional

weak expression: see section 9 7

thoracic ganglion

weak

regional

weak expression: see section 2 3 4 5

dorsal root ganglion

moderate

regional

moderate expression: see section 8 9 0 1 5 6 7 8

nasal capsule

weak

regional

weak expression: see section 8 9 0 1 2 4 5 6 7

stomach

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9

midgut

weak

homogeneous

weak expression: see section 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4263
Entity Detected:	Gap43, growth associated protein 43 ( MGI:95639)
Sequence:	sense strand is shown >T4263 AGAGGGAGGGAGAGGGAGGGAGGGAGGGAGAGCGCGCTAGCGCGAGAGAGCGAGTGAGCAAGCGAGCAGA AAAGAGGTGGAGAGGGGGGGAATAAGGAAGAGAGGAGGAAAGGAGAGAAGGCAGGAAGAAGGCAGGGGAA GATACCACCATGCTGTGCTGTATGAGAAGAACCAAACAGGTTGAAAAGAATGATGAGGACCAAAAGATTG AACAAGATGGTGTCAAGCCGGAAGATAAGGCTCATAAGGCTGCGACCAAAATTCAGGCTAGCTTCCGTGG ACACATAACAAGGAAAAAGCTCAAAGGCGAGAAGAAGGGTGATGCACCAGCTGCTGAGGCCGAGGCCAAG GAGAAGGATGATGCTCCCGTTGCTGATGGTGTGGAGAAGAAGGAGGGAGATGGCTCTGCTACTACCGATG CAGCCCCAGCCACCAGCCCCAAGGCTGAGGAGCCCAGCAAGGCAGGAGATGCACCTTCTGAGGAGAAGAA GGGTGAAGGGGATGCGGCCCCCTCCGAGGAAAAGGCCGGCTCAGCGGAGACAGAAAGTGCTGCTAAAGCT ACCACTGATAACTCCCCGTCCTCCAAGGCTGAAGATGGCCCAGCCAAGGAGGAGCCTAAACAAGCCGATG TGCCTGCTGCTGTCACTGATGCTGCTGCCACCACCCCTGCTGCAGAGGATGCTGCCACCAAGGCAGCGCA GCCTCCAACGGAGACTGCAGAAAGCAGCCAAGCTGAGGAGGAGAAAGACGCTGTAGACGAAGCCAAACCT AAGGAAAGTGCCCGACAGGATGAGGGTAAAGAAGACCCCGAGGCTGACCAAGAACATGCCTGAACTTTAA GAAATGGCTTTCCACGTTGCCCCCACCTGAACCCTGTCTCTCCTGCCCTTCTCAGCTCCACTCTGAAGTT TCCTCTCCTGTCCTGCTCACGTGTGTGAGCCTGTCCTCTCCTACCTATGAGCCCTCTCTCTCTGTGTGGC AAACATTTAAAGAAAAAAAAAAAGCAGGAAAGATCCCAAGTCCAACAGTGTGGCTTAAACATTTGTTTCT TGGTGTTGTTATGGCGAGTTTTTGGTAATGATGATGCAGTCATCTTGGGAAATTCTTGCACTGTACCCCG GTTTTTTGATCTGGTGCGTGTGGCCCTGTGGGAGTCCACTTTCCTCTCTATTTCTCTCTGTTCCAAGTGT GTGTGTGCAATGTTCCGTTCTGAGGAGTCCAAAATATTAAGTGAATTCAAAAACCATTTCTGTTTCCTCA TTTTCAATGTGATGGAATGAACAAAAAGGTTAAAAAATTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503501 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503501 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001126 same experiment
	EMAGE:21485 same embryo
	EMAGE:21483 same embryo
	EMAGE:21484 same embryo
	EMAGE:21482 same embryo
	EMAGE:21481 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source