EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21483

Lysmd4 LysM, putative peptidoglycan-binding, domain containing 4 ( MGI:1922349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001084_01	euxassay_001084_02	euxassay_001084_03	euxassay_001084_04	euxassay_001084_05

euxassay_001084_06	euxassay_001084_07	euxassay_001084_08	euxassay_001084_09	euxassay_001084_10

euxassay_001084_11	euxassay_001084_12	euxassay_001084_13	euxassay_001084_14	euxassay_001084_15

euxassay_001084_16	euxassay_001084_17	euxassay_001084_18	euxassay_001084_19	euxassay_001084_20

euxassay_001084_21	euxassay_001084_22	euxassay_001084_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21483_wholemount_strong.wlz

21483_wholemount_moderate.wlz

21483_wholemount_weak.wlz

21483_wholemount_possible.wlz

21483_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21483_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

weak

homogeneous

weak expression: see section 4 5 6 7 0 1

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 7 8 9

trigeminal v ganglion

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 7 8 9 0 1 2

vagus x ganglion

weak

homogeneous

weak expression: see section 8 7

vestibulocochlear viii ganglion

weak

homogeneous

weak expression: see section 6 7 8 9

dorsal root ganglion

moderate

regional

moderate expression: see section 7 weak expression: see section 8 9 0 5 6

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4264
Entity Detected:	Lysmd4, LysM, putative peptidoglycan-binding, domain containing 4 ( MGI:1922349)
Sequence:	sense strand is shown >T4264 GAGCGAGTTAAGAGAAAATGAGGCAGAAGGAAGTGTTAGCCAAGAGCTTCCAAGGTCCAGCTGCTGTCTG CAGGACTCCAAACAGCCACGTTTACATGTTCAACAATGGCAGCGGGGACTCTGGGGACTCCTCTGAGGAA GAGTCACATCAGGTGGTGTTACGACCCCGGGGCAAGGAGCACCAGAAGAACAGCAGCCAACGGCCAGGAG CAGGCACCATGGTTCTGCTGCAGCGGGAGCTGGCTCAGGAAGACAGCCTCAACAAGCTGGCTCTCCAGTA TGGCTGCAAACACTCAGAGTGATTAACAGTGGCAAAGTTGCAGATATCAAGAAAGCAAACAACTTCATCA GAGAGCAAGACCTGTATGCTTTGAAGTCAATCAAGATTCCAGTGAGAAATCATGGGATTCTCACGGAGAC CCACCAAGAACTAATGCCCCTTGGGGCTTCATCCTCTGAGACCAGGGTGACCCTGGTAGACCTGCCTGAA GATGAAGATGCTGGTGGAGCAACCACTCAGGGCAACCAGCTGACAGACTTCTTCAAGGGCATTGATGAGA ACATTGAGCGGGCTGTGCATTCTGACGTCTTCCACGGTGACAGCTGCTGTGTGGAGGCCCCAGATCAGCT GCTGCTCCCCATAACCCAGAAGCCAGTAGCCGATGGTGCCGACTGTGGGATTCAGTGGTGGAATGCCGTC TTCCTTATGCTTCTCATTGGGATCGTATTGCCAGTGTTCTATCTCGTCTACTTCAAAATACAGGCCACCG GGGAGCCCTCAAATGGCTTGAACGCAACTGTTGTCCCCAATGGCTCGATGACACTGAGCCCAGTTCCAGG GCAGGCTCCCAGGTTGGCAATCCCAGTGCCTACCCTCCCCGCTTCAGACAGCCAGGTCAGCCCTACCACC CAGGCAGGGGCCTAAGCGCATTGTTTCCTAGAGTTGGCAGACTTTGGCAGTTGGCCCTTGTGTAACTGTC ACCAGCTGTTTGCATTTCGCTTCCTGAGATGTGTGGACACCATGGCAATCTGGATGGTCTAGACTGAGGA ATAGCTTGGCCTAACTCAAGGATGCAGGAGCCCAGACATCCACCATCCCAGCAAGAGAGACATTTATATG AAACTCACAGCTCTTGAAGACATGTAGGGGCCGGCTGTGAGTTCCTGTGCAGCACTCTGTGCAGGCTCCT AAGTAGAATGTGACAAAGGTTCTGCACTATTGCTGCCCCCAGTGTTGAATAGGGTTTCAATGGTGGGCTC AGAGACATGCTCTGTGTCTAGATCAAGGTGCTATATAGTATGTCACTGAGTTTGTCAGTTGACAGCCTGA CATGTTATGTTTGCCTCTTCACTTGGCCCCACTGAGGCAGTGCTTCAGAAATGAGAACCTGAAATCCAAA CCGCCACCCTCTAATTTAAGCTCTACCCCCAGAGTCACCCTAAGAGCATCTCTGTGGTGGTGGCTCCCTG GGTTCCCCAGTTGGCATCTGGGAAGCAGCTGGTATTGGCGAATGGTTTGAAGGGACTACATCCACCATTA AGCTTCTGTCACATGGACCTTTGAAGCAAACTGGTTGCCATGCCAGCCCAGCATTGTTAATAACAGTGAG TCCCACCCCACCCTGATCATGTGCGCCCAAAGCAGAGTTCTCCCACTGCCTCCTTGACTCATCTATTCTT TGGGTCCTTAGAGTAAACTTGCACATGCTGTGTCTCAGTGTGAAAACCAGACACAATGTCAGTCAGCCGT CTTTTACTGTAACAAAATCATCTCCAGTGGGAAAGGTTTATCTTGGCTCATGGTTTCAATCCATGGCCTG ATTTGGGGCTGGGTGGCTGGGGGCTATGGTAAGAACACCATGAAGGAGAGTGTGGTAAAGCAGATTGTTC ATGTGGCAGGGAGAGAGAAGGGGAGAGGAGCTTGGGCGCAGGCATCACCTTCAGAGTTCTGCCTGCAGTG GCCTGACTTCCTCCCACTGGGTCCCATTTCTTAAAGGACCCATTCCTTCCCAACACTGAGATGAGGACCA AGCCTTTAAGATACTATCCAAACCCCACCAAGGTAACTCTGGCTCCGAAAGGCTGGAGAGAGAGATGTAG GAGGGTACAAAATGCTCAGGGTTTCCTCAGCCACTCCCTGAGAGCAGTTGGCCATCAGTCCTGTTAGGGA TGTTGGGGGGAGGGCTAAGTCAGCCTGCCTTGTCACAGCAGGGGCAGGCAGAGGTGGCTGGGAGCTCAGC CTGGGGAGGACAGGCTAGCAGGCTTCTGGGATGGTTAGCAACTTGACACATAGGCCTAGAAATCTGACCA GAGGAGCAGGACCTTACTTGAGCTGGGCCTCCTGTCTGAAAAACAACCCACCCAGGGTGCAGATAGCAAA TTTAAATCATCTTCAGAATTGAGTATTTTGAGGCAGAAAATGAACCCATGAATCTTGGTACAATAAACAT CTTGATTATGTAAGTGATTTCTATCTGGATGCTCAAAGACCGTTGTGGCTGTGAAGTTTCTCTTAAATGT TATCATTAACTTCATCAGGCAGGGCTCAGAGTCCCGTAGGGAAATGATTCTTTTGGCAGACAGAGAATGT CTATATTTTTTCTATCTTCTGTCTCAAGTGTTGGGGACTTATTACCACCTGTATATAGCCCTGCGTGGGT TCCTAAGTAAAAGGTGACAAGGGATCTGCAGTGTCAGGGCTCCTGAGGTTGGATGAGGTCAAATGACTAT CACAGTGTCCCTGCCTGCTGAGGGCAGGGGTGGGCTTGGCTCAGAGATTTGTGTTCTGTGTCTAGGCAGA CCATGGTGCTATGCAATAGTATCAGTATGTCACTGAGTTTTAGACATGTTACAGTTTTAACTTGTATTTC TGTATTTTTCTCAAGTGGAATAAAACAATTTCTTCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504057 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504057 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001084 same experiment
	EMAGE:21485 same embryo
	EMAGE:21484 same embryo
	EMAGE:21480 same embryo
	EMAGE:21482 same embryo
	EMAGE:21481 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source