EMAGE mouse gene expression spatial database

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EMAGE:21484

Nrm nurim (nuclear envelope membrane protein) ( MGI:2146855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001085_01	euxassay_001085_02	euxassay_001085_03	euxassay_001085_04	euxassay_001085_05

euxassay_001085_06	euxassay_001085_07	euxassay_001085_08	euxassay_001085_09	euxassay_001085_10

euxassay_001085_11	euxassay_001085_12	euxassay_001085_13	euxassay_001085_14	euxassay_001085_15

euxassay_001085_16	euxassay_001085_17	euxassay_001085_18	euxassay_001085_19	euxassay_001085_20

euxassay_001085_21	euxassay_001085_22	euxassay_001085_23	euxassay_001085_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21484_wholemount_strong.wlz

21484_wholemount_moderate.wlz

21484_wholemount_weak.wlz

21484_wholemount_possible.wlz

21484_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21484_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

weak

homogeneous

weak expression: see section 9 0 1 5 6

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

rest of cerebellum ventricular layer

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 5 6 7 8 9 0 1 2

midbrain ventricular layer

weak

regional

weak expression: see section 8 9 0 1 2 3 4 5 6 7

nasal capsule

weak

regional

weak expression: see section 8 9 0 1 2 3 4 5 6 7 8 9

chondrocranium

weak

regional

weak expression: see section 1 2 3 4 5 6 7 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4262
Entity Detected:	Nrm, nurim (nuclear envelope membrane protein) ( MGI:2146855)
Sequence:	sense strand is shown >T4262 CTCCCTGGGCAGGGCCAGCAGTCGCGGGAACCCTGAGCCCGCCTTCTCCTCAGGCTCCGCGCTGCTCGCC ATGGCACCGGCGCTGCTGCTGGTTCCTGCGGCCCTCGCCTCCTTCATCCTGGCCTTTGGCACCGGAGTGG AATTCGTGCGTTTCACCTCCCTGAGGCCGCTGCTTGGAGGGATCCCGGAGTCTGGCGGTCCCGATGCCCG CCACGGGTGGTTGGCTGCCCTGCAGGACCGGAGCATTCTTGCCTCCTTGGCTTGGGATCTATGTCTGCTG CTACTGTTTGTGGTACAGCACAGCCTTATGGCGACTGAGGCAGTGAAGGCGTGGACATCCCGGTACTTTG GCGTCCTTCAGAGGTCGCTCTATGTGGCGTGCACAGCCCTGGCCTTGCAGTTGGTGATGCGGTACTGGGA GACTACACCCAGAGGCCCAGTGTTATGGGAGGCTCGGGCTGAACCATGGGCCACCTGGGTACCTCTCCTC TGCTTTGTGCTCCACGTTGTTTCCTGGCTCCTCATCTTTAGCATCCTTCTGGTCTTTGACTACGCTGAAC TGATGGGCCTCAAGCAGGTTTATTACCATGTGCTGGGACTGGGCGAGCCCCTGTCCCTGAAGTCTCCTCG GGCTCTGAGACTCTTTTCCCACCTTCGACACCCAGTGTGTGTGGAGCTGCTGACTGTGCTGTGGGTGGTC CCCACCTTGGGCACCGATCGGCTCCTCCTGGCTCTTCTCTTTACCCTCTACCTGGGCTTGGCTCATGGGC TTGACCAGCAAGATCTCCGCTACCTTCGGTCTCAGCTACAAAGAAAACTCCACCTTCTCTCCAGACCTCA GGATGGGGAAGCAGAGTGAGGAGCTAGTTCTAAGCCCTGAATTTACTTCCCACCTCAAAATCAAATCCCT TAGTATCCAGACGGAGTTTGCTTCAGACCGCGGAGGAGGTTGGAAGTCATGCCCTGAAGGGGCTGTGCCC TCTCCTGCTGGAGAACTCAGTTCCTGAATCTTGATCCTGAATTCTTATTCAAAGTTTCCTCCACTGGTCT TCAAGATCCGCCTCTCACCAGCCAAGAATGGTTACAAAACTCATGAGTCTCCTTTTCTCTTAGAGCATGA CCTCCACACACACTGGGTCAGTTCACACAGGGGAGCACCTCAGCTCACACTGGGGAGCACTCACTCCCCT GGCACTGTTATTGGCTCTGCACCTCAGGGATTCCCTTCCATGTTTCCACCTCAGGGAGCTATGTCAGGGC CTATCTGAAAGTTTATAAGTAGTGGCTCTTTTGTACCGCGGCTGTGCTTCCTCCCCACTCCTGCCTTAGT TTCTCCTTAGGTCTTTCCCTACAGCTCTGTGTGGTCTCCTAAGGCGACGTCTCCCGATTTTAACTCGGGT TGACTCAGGGCTCCCTGCTCTCCCAAAGGATATGCGCTGCAGAAAAAATAAAATTTTATACATATGAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503599 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503599 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001085 same experiment
	EMAGE:21485 same embryo
	EMAGE:21483 same embryo
	EMAGE:21480 same embryo
	EMAGE:21482 same embryo
	EMAGE:21481 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source