EMAGE mouse gene expression spatial database

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EMAGE:21501

Sepn1 selenoprotein N, 1 ( MGI:2151208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003064_11	euxassay_003064_12	euxassay_003064_13	euxassay_003064_14	euxassay_003064_15

euxassay_003064_16	euxassay_003064_17	euxassay_003064_18	euxassay_003064_19	euxassay_003064_20

euxassay_003064_21	euxassay_003064_22	euxassay_003064_23	euxassay_003064_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21501_wholemount_strong.wlz

21501_wholemount_moderate.wlz

21501_wholemount_weak.wlz

21501_wholemount_possible.wlz

21501_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21501_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4665
Entity Detected:	Sepn1, selenoprotein N, 1 ( MGI:2151208)
Sequence:	sense strand is shown >T4665 GGACGACCAGTCCTGCTGAGGTTCCGGGCGGACTCTCCGGGAGACTGTCCTGGAAAGCCCGCCCATCCTC ACTCTCCTCAATGAGAGCTTCATCAGTACCTGGTCCCTGGTAAAGGAGCTAGAAGACCTGCAGACCCAGC AGGAGAACCCACTCCACAGGCAGCTGGCAGGCCTGCACTTGGAGAAGTACAGCTTTCCTGTAGAGATGAT GATCTGTCTGCCCAACGGCACTGTGGTCCACCACATCAATGCCAACTACTTCCTTGACATCACCTCCATG AAGCCTGAAGACATGGAGAATAATAACGTCTTCAGCTTCTCATCCAGCTTTGAGGACCCGTCCACAGCTA CCTACATGCAGTTCCTGAGGGAAGGACTCCGGCGGGGCCTGCCCTCCTCCAGCCCTAGTCCTAGAGGACC TGGACCTGGTAAAAAGCCAGCAGACAGAATCCACGCGCCTTGGAGCCACGGTGCCCGGAGTCTGTTTCAA ACTGGAAGTGGTCCCTACCTCATCCCCAGGCTGCCTAGAGAGGTCCAGGGTCATCTGAGGCTGTCACTTT CATAAATCCTGAGGGATGGTGGCCAGATGAGGGGGCCTGCCTGCCCGGTCATGTGACTGAGCTTAATCCG GCTACCACTAGCAGCCCATCTGGCTCCAAGAGCTTCTTCGAGTCCCCCCAAAAGGGGTCTGGGGAGACCT CCCTGGACCACACAGTGTAGTTCAGGACCTCGGTGTGAATAGGGAGTGGACTGGAAAGGTCCCTTCTTTC TAGGCTCCCCTTTCAAGCCAAAGGAATTAAAAGAGCTGGGCTCGGGGCCAAGCTGCCAGCGCTATGCCTG CTTGGAGCCTTCTATGCTGGCCATCCCTAGGTAGCCTTCTGTGCACAGTGCTCCCAGCTAGGGTCATTGT CAGCTGGAGACAGCTGTCTGGAGCCTCTGCACAAAGCCCTTCTACAGAAGTGGAGGCCATATTGTCACAC TGCTACCCTTCAGCCTGTTGACCTGATGACCCAAGTCCAGTCCTGTTCAGAGAGATGGAGAAGTTCACTG TGAAGTGTGTCCCAGCCAAAGAGCGCAGCCTTTCTTATTGCCAGCACTGTCTAGCCCCCCAGGGTACTGC CAGTCAACTCACCACCGGATGGCCACCAGTTCCCATCGGCTACTTTTGGGGACTTGGTGAACAGCTTGAA CGGCTGACCATGGCCAGTCTTTTTTTATGCATACAGAAAAGTGTTTTTATAAGAACTTTCTCACAGTCTA TATTTTTTAAAACTCTGAATGCTGAGGAGAAAGAAGTGGGCAGTGGCTCCCCCCAGCAACAGCCGCATGA TGGCTGGGTCTGAAATCGCAAATGGATCCAGCCTGATGTCCTCGCAGTTGCACATGTGGGGAGAAACTAA GCCCACCTTCCTTCTCTCCAGCTTTTTACAAGCAGTCAAGACTCAGTATTCCCCAGCCCTGTCCTGTGCT CCACACTCCCAAGAAGCGCCTTGAACTCAATCGCATCTGAGCCTTCTTGACATCAGCCAGGTCCCTGTGA GCCATCTTTTTACCTGGTCTCACCAGGGGCTCAAGCTTCAACCATGCAGAGGAGGAAGGCACATAGACAT TCCCTGATTCCTACTCCCCTGCCAGCCCTTGGGGCCACCACATAGAGCCCCCCTCACGCTCTCTGGCTCC CAGGAGCCTTGTCTCTGGGGTGCCCTGTGAAGATCACATACATTTTCTCATAGCAAACCATGAATTGGAA AGTTGCCCCCATTTTTCAGAGGAAGACATTGGAGTCTTAGGAAAGTAAAGTGACTTCCGGGGTCACTCAG AGGACCAGAGTTGTGATCCAAACCCACATGACTCTAAAGGCTATGTCTCGGTTTTGGGGGTGCCCTGGCT CATTTCCCCCTGTGGTGGGAAGGGAATGTTTTCAAATCATGTCTTCTTATCAGACTAAACAGAGCAGGTG TCTATAGCTCTGCCAGATTCTCTGAGCAGTCCTATGGGAAGTACTGATTGCTGGGGCTTTCTGGGTCTTC ATCAGACACCTCTGCACATCCCCAGCTGCCATCTGATCACCCCTCTGCCTTTGTTCCCCCATCCCCAGTA TGTCCTGTAATAAAATTGGAGAGCCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5012596 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5012596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003064 same experiment
	EMAGE:21504 same embryo
	EMAGE:21502 same embryo
	EMAGE:21503 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source