EMAGE mouse gene expression spatial database

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EMAGE:21503

Wdr4 WD repeat domain 4 ( MGI:1889002)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003073_01	euxassay_003073_02	euxassay_003073_03	euxassay_003073_04	euxassay_003073_05

euxassay_003073_06	euxassay_003073_07	euxassay_003073_08	euxassay_003073_09	euxassay_003073_10

euxassay_003073_11	euxassay_003073_12	euxassay_003073_13	euxassay_003073_14	euxassay_003073_15

euxassay_003073_16	euxassay_003073_17	euxassay_003073_18	euxassay_003073_19	euxassay_003073_20

euxassay_003073_21	euxassay_003073_22	euxassay_003073_23	euxassay_003073_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21503_wholemount_strong.wlz

21503_wholemount_moderate.wlz

21503_wholemount_weak.wlz

21503_wholemount_possible.wlz

21503_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21503_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4684
Entity Detected:	Wdr4, WD repeat domain 4 ( MGI:1889002)
Sequence:	sense strand is shown >T4684 CCCACGCGTCCGCGGAGGCGGCGGGCGGGCCCATGGCGAGCTCTGCGGGGCTGGCACTGTGCGCCCAGAC GCTGGTGGTGCGAGGAGGCAGCCGGTTCCTAGCCTTCTCCACTACGGGCAGTGATGATGACTGTGTCTTC ACATACGACTGCAGTACTGCAGAGAAGAAGGCCACGCCAGAAGATAAAGGGGAGGACGGACAGCCCGCAG ACACAGGGAGTGACTCGATTCTGGCGTCCACCTTCTCCAAGTCTGGCCGCTATTTTGCTTTAACAGATGA CAGTAAGCGTCTGATTCTTTTCCGTACAAAACCATGGCAATGTCTGAGTGTCAGGATGGTGGTGCGGAGG TGCACCGCCCTGACCTTCACAGCCTCAGAGGACCGAGTCTTGGTGGCTGACAAGTCTGGAGACGTCTACT CCTTTTCGGTGCTGGAGCCAGATGGATGTGGCAGGCTGGAGCTTGGGCACCTCTCCATGCTGCTAGACGT GGCTGTGAGTCCTGATGACCAGTTTGTGCTTACTGCAGACCGGGATGAGAAGATCCGGGTCAGCTGGGCT GCTGCCCCGCATAGCATCGAGTCTTTCTGCCTGGGACACACTGAGTTTGTGAGCCGCATCCTTGTAGTGC CCAGTCATCCTGAACTGCTGCTTTCTTCCTCTGGGGATGGCACCCTGAGACTCTGGGAGTACAGAAGCGG TAGGCAGCTGCAGTGCTGTGACCTGGCCGGCCTACAGGAGCCTGGAGAGCAGCCAGGCCACAAGGGGTTG GCCGCGTCCAGGATTGCATTCTGGGGACAGGAGAGCTATGTGGTGCTTCTGTGTGAGTGCGTTCCCGTGG TCTTCGTCTTCCAGCTTGATGCCAGCAGACAGCAGCTGGTGTTCAGACAGCGGCTGACTTTCCCTCACCG AGTGTGGGATGTTGTGTTTGAGGAGGCCCGGGGGCTGTGGGTTCTACAGGACTGCCGTGATGCCCCCCTG GTGCTCTGGAGGCCTGTGGGTGGTGAGTGGCAGGCTGCTCCAGACGGTGCTGTGTCCCCGAGACTCTGCA GCCATCTCCGTGAGAGCTGGGCCATGCTGGAAGGTTCTGTTGGTACAGATGACAGCTTCCGCAGCCTGTA CAAGGCCACCTTTGACAACATGACCTCTTACCTGAAGAAAAAGGAAGAGAGACTGCAGCAGCAGCTGAAG AAGAAGCGGCAAAGGAGCCCCTTCCCAGGGTCCCCGGAACAGACCAAAAAGGCGTGCCCGGGCCAGTCAG CCCTTAGTTGCTGACCTTGGACATGGCCACAGCTCTCATTCCTGAGGCCCTGTGGGCAGCTCTCTCCCTC CTCCTCCCTGGTTCATCCCTAGGGAGGGAGATCACGGCAGCTCAGTCCCTACCACTAGCTCAACAGTCCC TGATCTCCAGAGTCTGCCTTTGTCACTGAGAACAGGCTCCTGCCGGGTTGCAGGGAAGGCAGGTGGTGTT AGCTTAGCTAAGAGTCTAGAGCTGTGTGGAAGCACCTTCACATTGAAGGATACACTGGCCCACAGCACCA CGCATGTCTGTCGGGGCCCACAGTGTCGTCCCTGGGGAGCTCTAGGGAGACCTGCAGGACAGGACCTGCC TTCACATCATTGCCGGGGGCTCTCCAGGTGGAGCTCTGTATGGCTCTGTAGCTGCTTGCTGTTTTGAGGC AGATTCAGTTGGTGCTGTTCGCACTCCTGGGCTGGGCTGAGCAGACTGAGCAAGCTTTGGGGCTCTGAGG GTGTGACCTTGCCAGGCCAGCCTTGTTTTACAGGGAATGATGGTGACCTCGTGGTTCTGTTGCTCTGAAC TCTGCATACACTGCAGAACAGAAGGAAATGAACAGAAATCACAGCATCAATAAAGACAAACTTTGCATAG CTTTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5027600 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5027600 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003073 same experiment
	EMAGE:21504 same embryo
	EMAGE:21502 same embryo
	EMAGE:21501 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source