EMAGE mouse gene expression spatial database

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EMAGE:21521

A130010J15Rik RIKEN cDNA A130010J15 gene ( MGI:2441776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000996. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000996_01	euxassay_000996_02	euxassay_000996_03	euxassay_000996_04

euxassay_000996_05	euxassay_000996_06	euxassay_000996_07	euxassay_000996_08	euxassay_000996_09

euxassay_000996_10	euxassay_000996_11	euxassay_000996_12	euxassay_000996_13	euxassay_000996_14

euxassay_000996_15	euxassay_000996_16	euxassay_000996_17	euxassay_000996_18	euxassay_000996_19

euxassay_000996_20	euxassay_000996_21	euxassay_000996_22	euxassay_000996_23	euxassay_000996_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21521_wholemount_strong.wlz

21521_wholemount_moderate.wlz

21521_wholemount_weak.wlz

21521_wholemount_possible.wlz

21521_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21521_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0 7 8 9 0

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4339
Entity Detected:	A130010J15Rik, RIKEN cDNA A130010J15 gene ( MGI:2441776)
Sequence:	sense strand is shown >T4339 GTGGGAGGGCACTACGCCGCTGGCGGGCAGGCGGAAGGGCTTCCTGCGCCCCCGAACCTGCGGCGGAAAT GCTAGCCGAGTGTCTTGAGGCAGAGCGCTTCCTTTTGTAACTAGTCTACCACCGCGTAGGCACGTGTGGG ATCTTTCTCAAGTGCTGGGAGCTGAACTGTGTGTGGCCCAGGGAAAGGAACTGTGGCTAGCTAAGGAGGC CATATGTCATGTAACTTCCAGATCTTATGTCAACACCAAGCCCTCAGCTGCTGGTGGCAGCTGCTCAGCA GACTCTGGGCATGGGCAAGAGAAAATGCCCACCTCGAGCTACCTGCCTTCACCTGGCAGGAGAGGTGCTG GCTGTGGCCCGCGGGCTGAAGCCAGCTGTACTCTATGATTGCAACTCTGCAGGAGTATTGGCGCTCCAAA GTTATCTGGAGGAACTACAGGGGCTGGGCTTCCTGGAGCCAGGACTTCACATTCTTGAGATTGGAGAGAA TAACTTTATTGTCAGTCCTGAGTATGCCTGTCAGCATTTGGAACAGACACTGCTTGGTACTGTAGCTTTC GTGGACGTTTCCCGCTCCCAACCTCATCCTTCTGTCCGGTCGGTGGACCAGCTCCCGGACTTGAAATCTC TCATAGCTGACGTCATCACACGTTTTCGAGGACTTAAGAAAGATGTGTCTCAGGGAGTCTCCTACACCAG GCTACATTCCTCAGACTGGAATCTCTGCACTGTATTTGGGATACTCCTGGGATATCCAGTCTCTTACACA TTTGACCTGAACCGTGAAGATGACAACTGCTTAACCATGACTCCCCTGCGGGTGTTTACTGCCCGGATTT CGTGGCTGCCTGGTCAACCATCAATTCTGCTGTACTCCTTTAGTGTTCCAGAGAGTTTGTTCCCAGCCCT GAAAAACTTTCTAAGTGCCTGGGAGAAGGAACTCAGAACCCGATTTAGGGCTCAGAATGCCTTTGCTGAC CTCAGCATCTCCTCTGAGGTGGTCACACTTCCTGCTGTGGCCCTCTGAGTTAACTCTGTCCGTTGAGAAG GTATCAATGAATGTCAATTACGATGAATGTTGTGCAGTCAATTGAACACGTGTGAATGGGGAGAGTCGTT GGCAGCAGTAGTATGATTTCAGTCTTTATATTAGGAGTTCCTAGGTCCTTAGCCGAGGTGTCTGTTTATT TTTCATTGAATGAGAGATGGAAAAGGAGCATTGTGGAGACATGAGTGTACCCTGGGATGGTGGCCTTCCC TGGTGGATAGTGCCTTAGAGGGAAGGAGAATGACTAGGTAATCTATGTATGGAACTCTAACTGAAGTAGG GTGCGAGACAATGATGCTTAAGGGTTACACTTGTGGCTTATTTCTCAGGAATACACAGTTCTAAAGAGTA GTCTTGTGAGATAAAGTTTATTTACAACCTGGTGAAAAAAAAATAAAACTAAGAGTCCGGATTCATTTGG AGGGAAACCCAAGTTATAAAGTACCAGAACTCAAGGTTTTCATGGAGTTGTTTGCATCTGAGTTTTACTT CAGATAGATGCCAGAAGTTCTTCAGATGACCAGGTTGCCTTTGTTTGCCAGGAACACTGCCAGTGCTGTA GCAATCACCACCATCAGCACTGGGAGCCATTGACCACACTGCAGCTGTATGGAAGGAGGTGGAGACAGGA GAGAGCAGCTATGAATTAGCTGGAGAAAGTATGGGCAAGAATAGTCCTTTGGCATTTTAACCCAGTAGAA GACCTCACACTGGTGGCAGAAGGGGCTTTTTTTTTTTTTTCTCTTCAGTGTCTCCCTCACTTTTTGTCAT TTCCACTAGATAAAACCAGTTTACTAGTGGCCTAGAGACTCATATCTGACAGGGGACAGATATTTAAATG CTGATCTGAATGAGGTGGAAATTAACTCAGCAGAATAACACTCTTTGTACACGCCTCAATACTTTTGCAG TCCTCCTCTGCCCTTGTCATTATGTTTACAATAAATCAAAAAAGTACTCTTTCATGCTTGAACCAATACA GGCTGAATATCCTTTTTCTGAAATGCTTGGGATTAGGACTCATTCAAATTTTGTCTTTTTTTTTCCCATT AGTATTTGTTTAGTATTTGTATTAGTATTTGTAGTAGTAGTATTAGTATTTGTTTACCAGTTGAGCAGAC GTAATAAAAAGACATCTGAATACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527064 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527064 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000996 same experiment
	EMAGE:21523 same embryo
	EMAGE:21520 same embryo
	EMAGE:21522 same embryo
	EMAGE:21519 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source