EMAGE mouse gene expression spatial database

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EMAGE:21523

Gabrg2 gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 ( MGI:95623)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001003_01	euxassay_001003_02	euxassay_001003_03	euxassay_001003_04	euxassay_001003_05

euxassay_001003_06	euxassay_001003_07	euxassay_001003_08	euxassay_001003_09	euxassay_001003_10

euxassay_001003_11	euxassay_001003_12	euxassay_001003_13	euxassay_001003_14	euxassay_001003_15

euxassay_001003_16	euxassay_001003_17	euxassay_001003_18	euxassay_001003_19	euxassay_001003_20

euxassay_001003_21	euxassay_001003_22	euxassay_001003_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21523_wholemount_strong.wlz

21523_wholemount_moderate.wlz

21523_wholemount_weak.wlz

21523_wholemount_possible.wlz

21523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

homogeneous

not examined expression: see section 2 3 4 5 6 7 8 9 0 1

brain

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

facial vii ganglion

moderate

homogeneous

moderate expression: see section 3 4 6 0 1 2

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 6 7 9

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 2 3 4 5 6 7 8 7 8 9 0 1 2

vagus x ganglion

weak

homogeneous

weak expression: see section 8 9 7 8

spinal cord

moderate

homogeneous

moderate expression: see section 0 1 2 3 4 5 6 7 8

dorsal root ganglion

moderate

regional

moderate expression: see section 7 8 9 weak expression: see section 8 9 0 1 2 3

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4277
Entity Detected:	Gabrg2, gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 ( MGI:95623)
Sequence:	sense strand is shown >T4277 GTATCTGCAAGCGTTTGTACTGATCTTTATTTCTGCCCCCTGAATATTAATTTTTAAGCTGGTAGCAATA CATCTCTGAGTGACGGGACCTACTAGAGGCAGGTGGGGGGAGCCGGCATCAAATCATCAGCATAATAATA ATACAAAGGGGAGGGATTCTTCTGCAACCCAGAGGCGAGAGGCGAGAGAAAAAAAAAAAGCGATGAGTTC GCCAAATACATGGAGCATTGGAAGCTCAGTCTACTCTCCTGTATTTTCACAGAAAATGACGCTGTGGATT CTGCTCCTGCTATCGCTCTACCCAGGCTTCACAAGCCAAAAGTCAGATGATGACTATGAAGATTACGCTT CTAATAAAACATGGGTGTTGACTCCAAAAGTTCCAGAGGGTGATGTCACTGTCATCTTAAACAACCTGCT GGAAGGGTATGACAACAAACTTCGACCTGACATCGGAGTGAAACCAACATTAATTCATACAGATATGTAT GTGAACAGCATTGGTCCAGTGAATGCTATCAATATGGAATATACAATTGATATTTTTTTTGCCCAAACCT GGTATGACAGACGTTTGAAATTTAACAGTACCATTAAAGTTCTCCGGTTGAATAGCAATATGGTGGGGAA AATCTGGATTCCAGACACTTTCTTCAGGAACTCCAAAAAGGCTGATGCTCACTGGATCACCACTCCCAAC AGGATGCTGAGAATTTGGAATGATGGTCGAGTTCTCTACACCTTAAGGCTAACAATTGATGCTGAGTGCC AGTTGCAATTACACAACTTCCCAATGGATGAACACTCCTGCCCCCTGGAGTTCTCCAGTTATGGATATCC TCGTGAAGAAATTGTTTATCAATGGAAGCGCAGTTCTGTTGAAGTGGGTGACACAAGATCATGGAGGCTG TATCAATTTTCCTTTGTTGGATTGAGGAATACAACTGAAGTAGTGAAGACAACTTCTGGTGACTATGTGG TGATGTCTGTGTACTTCGATCTGAGCAGAAGAATGGGCTACTTCACCATCCAGACTTACATTCCCTGCAC ACTCATCGTGGTCCTGTCCTGGGTGTCCTTCTGGATCAATAAGGATGCTGTTCCTGCCAGAACATCTTTA GGAATCACTACTGTCCTGACCATGACAACTTTAAGCACCATAGCCAGAAAATCTCTGCCCAAGGTCTCCT ATGTCACAGCAATGGATCTCTTTGTATCTGTTTGCTTCATCTTTGTGTTTTCTGCTTTGGTGGAGTATGG CACCCTGCATTATTTTGTCAGCAACCGGAAGCCAAGCAAGGATAAAGACAAAAAGAAGAAAAACCCTCTT CTTCGGATGTTTTCCTTCAAGGCCCCTACCATTGATATTCGTCCCAGATCAGCAACCATTCAAATGAACA ATGCCACACACCTTCAAGAGAGGGATGAAGAATATGGCTATGAGTGTTTGGATGGCAAGGACTGTGCCAG TTTCTTCTGCTGTTTTGAAGATTGCCGAACAGGAGCCTGGAGACATGGGAGGATACATATTCGCATTGCC AAAATGGACTCCTATGCTCGGATCTTCTTCCCTACCGCCTTTTGCTTGTTCAATCTTGTTTACTGGGTCT CCTATCTTTATCTGTAAAGAGGTATGGGTTTCATTGATAGGGGTGTTATTCGCTGAATCTTATGGAGACA AAATGCACTTTCTAAGTCCAACGATATAATCCCCTATGTGGTCACCGAATGTGTTTCTCTGTCTCAACGT AGTAATACATATAATGTCATACTATCCATGCCCAATCCGTGGTTATTGTAATTTGAATTTCAGCGTGCAC TGTCCTTCAAACCTGCAAGGCAAAGTAAAGTTAGAACAAGAGCTGTTACACTGAGCAAGATACCTTTGAG CAACAGCAATGAAAACAGTGGAAGCTGGGAGGGTTTAAAGTGGCATTATCAGTCTTTGACTCATATATAG TTTAGTACAAAAGGTATAAAGCTGTGTACTATGTTCACTTGGGATCAAGTTGTGGTAAACTGGAAAAAAT TAGAATACCTCCCGTATCTGTGAAGAATCACAATAGAAAAGCCTAGATTAGAATTCTATTATACCTTTAT TCCAAGGTAGAAAGCAAATTTCCTTCATATCATATGTACAGTGATGTAAATATTTCAGTAATGTAGAATG CTTCAGGTTTAATGCATGCATCTCTTTAGAGCCAAAATAAATGAACTCATGTTATCATCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504205 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504205 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001003 same experiment
	EMAGE:21521 same embryo
	EMAGE:21520 same embryo
	EMAGE:21522 same embryo
	EMAGE:21519 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source