EMAGE mouse gene expression spatial database

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EMAGE:21573

Micall2 MICAL-like 2 ( MGI:2444818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001230_01	euxassay_001230_02	euxassay_001230_03	euxassay_001230_04	euxassay_001230_05

euxassay_001230_06	euxassay_001230_07	euxassay_001230_08	euxassay_001230_09	euxassay_001230_10

euxassay_001230_11	euxassay_001230_12	euxassay_001230_13	euxassay_001230_14	euxassay_001230_15

euxassay_001230_16	euxassay_001230_17	euxassay_001230_18	euxassay_001230_19	euxassay_001230_20

euxassay_001230_21	euxassay_001230_22	euxassay_001230_23	euxassay_001230_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21573_wholemount_strong.wlz

21573_wholemount_moderate.wlz

21573_wholemount_weak.wlz

21573_wholemount_possible.wlz

21573_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21573_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0 7 8 9 0

vibrissa

moderate

regional

moderate expression: see section 7 8 2 3 weak expression: see section 9

dorsal root ganglion

weak

regional

weak expression: see section 7 8 9 0 1 2 6 7 8 9

liver lobe

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

chondrocranium

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 0 5 6 7 8 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4671
Entity Detected:	Micall2, MICAL-like 2 ( MGI:2444818)
Sequence:	sense strand is shown >T4671 ACCTGGGCCAGCTCCCTGCCTCATGCCACACGCTCCTGCTCCACCTCCGAGCCTGGGTTTTCCACAGAGT TTAAGTAGGTGCCTGCCCACAAGGACCATTTCCGCAATGAAGCCACCCTGCGGCTGCCAGAAACTTCAGT GCCCTCCGGAAGGAAAACATTTACGAGAACAACAAGTTGGCCTTCCAAGTGGCTGAGGAACAGCTGGGCA TCCCAGCCCTGCTAGACGCTGAGGACATGGTGGCCTTGAAGGTTCCAGACCGGCTGAGCATTCTCACCTA TGTGTCACAATACTACAACTACTTCCATGGCCGGTCCCCAATTGGGGGCATGGCTGGCATAAAGAGACCC TCATCCGATTCGACCGAAGAACTTTCTGGAAAGAAGGGCCTGTCCCAGCCTGCCAAGTTGCCCTCGCCGG CTCAAACCCAGAGGTCACCACTGTCCCCAGCTCGGACAAACCCTGTGGTTCAGAGGAATGAAGGTGGCTC ACAAAGACCATCCCCAAAGGCTGCTCCGGGGACCGCGGGAAGCTCTGTCAGCAGTATCTGTGGGGTCTGT GGCAAACATGTTCACCTTGTGCAGCGGCACCTGGCTGACGGGCGCCTCTACCACCGAAGCTGCTTCAGGT GTAAACAGTGCTCCAGCACGCTGCACTCGGGGGCCTACCGTGCCACGGGAGAGCCCGGAGTCTTCGTCTG TACCCACCACAGCTCAGAAGTCACCTCTGTGAGCCCCAAGTCATCAAACCTGGCCTCCAGGAAACCAGGG GGTGTCACTGCAGACACCAGGTCAGTCGGCGTCTCATGGACGGTTCAGGAGGCAAATGGAGAGGGGACAC CACTGAGGGTCAGGACAGCAGCATGGGAACACGCTGGAGGAAACACAACCGCCAAAGGTTTTGTCCAGAC GGAACTTAAGCCACCATCTACCTCCCAAGTCCACGTGGGGAGCTCTGCGGGGCCCAAGCTACCCACGAGC ACAGTGACTACTACTTCTGTGACTAGCAAAGCTCTCACCCATGTGACGAATAGCTCCCCAACAGGATGGT CATCGCCTGCCCAGAGCAGCCCAGCGAACTTCAACTCCCGCCCAGTTGTATCCCCAAGTGCTCGGAACAC TCACCTACCTGGGTCCCAAGGGCAGACAGCCTCAAAAGGTGTTAAGACTCAGCTCAACTTGAACTCAGAG TCTTCAAACACAGCGGTCACCCCAGCCTGGACTTCCTCAGCCTCTAAGACCCAGCAGGCCCGAGAGAAAT TTTTCCAGACCCCTCCCTCGGCCCCAGCCCCAGCCTCAGCCCCAGCCCCAGCCCCCACCAGCAAGGTCCC CACTGTAGTGACTGTCCCCACCAGCAAGGTCCCCAATGTGGTGACTGCCCCCACCAGCAAGGTCCCCACT GTAGTGACTGTCCCCACCAGCAAGGTCCCCACTGTGGTGTCTGCCCCCACCAGCAAGGTCCCTACTGTGG TGTCTGCCCCCACCAGCAAGGTCCCCACTGTGGTGAATTCCACTAATAGCAGGGTCACCACGGTAGTAAA TGCCCCCACCAGCAAGGTCCCCACTGTAGTGAGTGCCACAAATGGCAGGGTGCCCACTGTGGTGACTGCC CACACTGGTAGGGTCCCTGCTGTGATGAATACCTCTGCCAGCAAAGTCTCTCCTGTTGTGGATGCCCCTG CCCAGGAGAGCAGCCGGGAGCAAGCATTAAGTGTCCTTAGGAAGGCCCTTCCGGCGCTGACAGGATCTGG TACCCAGGCTCCAAACAGGTCCTTCCCAGCCACTTCCTCTGTCCTGGTAACCCTTCCCAAGAATGAAGTG CCACAAAAAGTTCCCTCAGACAAGCTCTCAGCTTTAACCACCCAGACTCCTAATTTCACTATAAAGCTAG AGCCTTCAGCCCCAGTGAATGTGGGCAATACCGCTGTATTTCTCCAGGCTGGCAAAAAGAGCCCGAGTAT TTCTCCAAGGGTTGGTAAGACCAGCGTTGGCTCCAGGCCACAAGCAGAGGTGGCAGGAGTAAAGGGTCCA GGTCCCATCTCTCAGGAAGGCCAAGAGGAGGGACCAGAAGGATGGAGGGCCCGGCTGAAGCCTGTGGATA AGAAAACCCCTGCTGGGAGGTCTCTGGAGCAAAAAGAGCCAGTCCTGGCAGAGCCAAGGATAGGTGACAC TTCCAGGAAAGCCTCTAGTAGCTCTGACTCCAGCGTCCATATCACCTTGACTTCTATTCAGCACAAGAGG AAACCATGCCCTGCTGGTTCCGGGCCCAGTCCTGCAGCCCTCTCTCCTTCTCCATCACACCGCAAGAAAC TAGCTGTCCCTCCCAGCCTTGATGTTTCAGCTGATTGGCTCCAGCCAGAGCCCAAGAAGCAGGAAGATGG GACCAGGAGCTGTAAGGAGGAGAAGTCACCCACCAGGTGGTCAAGAGAGAGGTCTGCAGTCCTGGACAGT GGCCTTGCTCCACCTGGTGAAGCTGTCACTTCCCCAGTCAGGCTCCACCCCGACTATATTCCCCAGGAGG AGCTGCAGAGGCAGCTGCAGGACATTGAGAGCCAACTGGACGCCCTGGAGCTCAGGGGTGTTGAGTTGGA GAAGCGGCTGCGGGCAGCTGAGGGAGATGCTTCTGAGGATAGCCTCATGGTGGACTGGTTCCGGCTTATT CATGAGAAGCAGCTACTGTTGAGGCTGGAATCGGAACTCATGTACAAGTCCAAGGACCAGCGCCTGGAGG AGCAGCAGCTGGACCTCCAGGGTGAGCTGCGCAGGCTGATGGACAAGCCAGAGGGTCTGAAGTCCCCCCA GGACCGCCAGCGGGAGCAGGAGCTGCTGAGCCAATACGTGAACACCGTGAATGACCGAAGTGACATCGTT GACTTCCTGGATGAGGATCGGCTCCGGGAACAGGAAGAGGACCAAATGCTGGAGAATATGATCCAGAACC TGGGCCTCCAGAGAAAGAAGTCCAAGTCCTTCTTGTCCAAGATCTGGTCCTCGAAGAGCAAAAGCGGGCA GGCCTGAGCTGCACGTGGCTGGCACTGGGCCCTGGACCTCCTCAGGAAGAGACTGGTGCTGCGGTCCCCG AAAGGGATACTGGGGGTGCCAGGGCCTGGGCCTCCCACTCTGGGTCCTCGTCACCTCAATAAAGACACTG ACCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5025620 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5025620 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001230 same experiment
	EMAGE:21569 same embryo
	EMAGE:21572 same embryo
	EMAGE:21571 same embryo
	EMAGE:21570 same embryo
	EMAGE:21574 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source