EMAGE mouse gene expression spatial database

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EMAGE:21606

Pphln1 periphilin 1 ( MGI:1917029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004340_01	euxassay_004340_02	euxassay_004340_03	euxassay_004340_04	euxassay_004340_05

euxassay_004340_06	euxassay_004340_07	euxassay_004340_08	euxassay_004340_09	euxassay_004340_10

euxassay_004340_11	euxassay_004340_12	euxassay_004340_13	euxassay_004340_14	euxassay_004340_15

euxassay_004340_16	euxassay_004340_17	euxassay_004340_18	euxassay_004340_19

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21606_wholemount_strong.wlz

21606_wholemount_moderate.wlz

21606_wholemount_weak.wlz

21606_wholemount_possible.wlz

21606_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21606_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5894
Entity Detected:	Pphln1, periphilin 1 ( MGI:1917029)
Sequence:	sense strand is shown >T5894 GGCGGCTGAGGCGGCGGCGACAGAGGCGGCGGCGACAGAGGCGGAGGCTCAGCAGTGGGACGTAGTGGCT TCCACAAGAGATGAAATGTGGTCTGAGGGACGCTATGACTACGACCGACTTCCGAGAGAACGCGTGCCTC CCCGGAGCCATCCCAGTGATGGCTACCATAGAGTAGTTAATGTTGTGCCAAAGAGACCACCTCTGCTAGA CAAGAGACCACCTCTGCTAGACAAGAGACCACCTCTGCTAGCCAGACCTGATGAAGGAGGCTACAGTAGA TATTACAGTCATGTTGATTGCCGAGTATGTGACGAGGGCCGCAGTTTTTCTCATGATCGAAGAAGTGGCC CATCTCACAGTGGAGATGAATCTGGCTACAGATGGCTAAGAGATGATCATTCCACAAGCCGGCAGCCTGA CTACAGGGACATGAGAGACGGCTTCAGAAGAAAGAGTTTCTACTCTTCACATTATTCGAGAGATCGGTCT CCCCATAAAAGGGACGCTCCCTTCTTCAGAGAATCCCCTGTCGGCCGGAAGGACTCCCCACACAGCAGAT CCGGCTCCAGTGTCAGCAGCAGAAGCTATTCTCCAGAGCGAAGCAGGACTCACTCCTTCCATCAGTCTCA GCATAGAAAGTCCTCGCGTGTCGGTGCTTCCTACAAACGACAGAATGAAGCAATTCGTGGAAGAGGTAAA GAGAGATCCATCCAGTCAGTGAAAACCTCGAGAGATGCGTCACCCTCAAGTTCCTCAGCAGTTGCTTCAT CCAAGGCGTTAGACAAACCCAGCAGGCTAACTGAGAAGGAACTTGCTGAGGCTGAAAGCAAGTGGGCTAA TGAAACACTAGAGAAGTCAGACGAAAGTAACTTGGCTGAAATGAATGAGTTTGAGGCGGGATCCACGGCA CCCTTATTTATTGACCAGACAGAAGAACCCGAGTCAAACACAGTAGATGGTACAGAACTGTATGAAGACA GCCAGCTCAGCAACCGCTCTAAAGCCATTGCCTCGAAAACCAAAGAGATTGAGCAGGTTTACCGACAAGA CTGCGAGACTTTCGGGATGGTGGTGAAAATGCTGATTGAAAAAGATCCCTCATTAGAAAAGTCTGTCCAG TTTGCACTGAGGCAGAACTTACACGAGATAGGTGAGCGCTGCGTGGAAGAGCTCAAGCGTTTCATTACTG AGTATGACAACTCTGCTCGGGACTTTGGAGACCCCTTTTAGAAGAAGAAGTACCCAGGTCAAAAGGTTTT CTTAGATTGTTTTTTTCTCCTTGGATTGTGTAAATCCTTACTATTTTGTGATGAAGAGAAATACTTTATG ATAGCTAAGCACATTTCTAGTACTAAATAAACATCTACAATAGCTTAAAGTATCTTAATTAGAATGTTTC CCCTATGTGTAGAATATTCTACTCTATCTTCATACATTTCCTCCCTCCAAAAATAATATTCAGTTACTTT AATGTTCAAAAGAAAAAAGCAGCTCTGGAGAGCAGCTCCCTGTATTTAACCCATGTTTCATAGTCTTCCA TGACGTCTATGATCATCTTTTTGCATACCGTGTAAAGGTTGTCTTTTTTATTTTGTTTTGTGTTTTTTGT TTTTTTGTTCTTTTTTTTTCGAGACAGGGTTTCTCTGTGTATCCCTGGCTGTCCTGGAACCCACTCTGTA GACCAGGCTGGCCTCTAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCA CCACCACTGCTGTATTCAGATGTTGATTTCCAGCTGAATACTCTTTGGTATTGTTATTTGAATTGCTAAG CACCTTCATTGTAGTTTGTAAACTCTCCTCTCCACCACCTTCTGATTGGTAAACACTGAGGGCCTGTAGG TCAGGAAAGGAAAGGCAGGGCAGGGCAGGGCATCACAAAAGGAAGTCTGACTTTCAGGGACTGAAAGATA ACGGGCCATGTGACAAACATGGACCAAAATAAATGGATTAGTTAAGCCATAGGAGCTAGTTGGGAAGAAG CCTAAGATATAAGGCCTTGGCATTCATAAATAATAAAAAGTCTCTGTGTTATTGTTGAGAGTCAGGGGTC GAAACAGTCTAACAGTAGTGATCATTTGGTCTCAGGAGTTTAATTTGTAGTTAAGATCATTCCAGATTGC ATACAATTAGTGGTACCGTTAGCATTTTGAGTTGGTGATGTCAAAATATGCTGTCTTAATGAGTTAGAAC TTTGCTATCATTCAATATTTGCTTTGAGAATAATTCTTATTCAATATACAAAAAAATGCACTTGAAAATC TGTAAGGATCTGTTAGAACAAAATCTTCTCCCAAGTCAGTAAACTTGGTTCATCTCTTCTATGAAGTGCT CTTAAGGCTTCTACCTGCTCTCTCTAGACAATATTATGGACTAACAGGAGAGGTAATCTGAGATGCAAGA TGAATATTAAGTTGAGATGGTCTCAGAAACCCATCTCATGGGTATAATAAAAAAATTAGAATATACAGAG CACTTAAGTTATGTTTTAGGCCCACATTATATCAAAAACGGGACCTGACATGGCACTCATGCTTGGGATT AAGCTATAGATTACTCTGTCCATCTCCGTGATCTCAGTTCTGTGGGCGTAGAGGGGCTCACTGCTCACAA GTGTCCTCAGAACACACTGAGGCCAGCACAGCTGCCAGCAGCTCCTGAGTGAGCCACTGAATCAGTGAAG AGACACTTAGGATCTCTTGCTCACTTCCTGGTCCCTGGTTCACTGAGGCAAAATACTAAATTTGTGACAT TAGGCACCTTAAAAAAAATCACTGCCCAATCAAATTATCTACCTTTTTTTTGGGGGGGGGGGGTCAGGGT CTTACAGTATAGTCCTAGCTAGCCTGAAAATCTCTGTGTAGACTAGGATAGCCTTAAACTCACAGACATC TCCCCACCCCTACCTCTTATATGCTGGCGTTAAGCCCACGGGCCACCCTGCTCAGCATCCTTTTGTTTGT TTTCATTTTCATTTTTTTTCTGTCCTTATTACTTTTTGATTCGAGACAGGGTTTTGCTGTGTGTCCTAGG CTGGCTTCAAACTTTCCTGCTCTGGCCTTTAGCTTTCGGGATTCCTGCCTCAGCTTTCCTGAGCCCCAAT GCTTGCCGTGACCATTGAGTGGCCTCTATGATCCTGTCTGCGGATATCCCTAAACCACATACAGATTATA CTTAAGTAGAAATAGAAGAATGGTTAATTTTAATGAGCCCTAAAATAGATGTGTGAAGATTTGTATTATG CCATTTTGAAAATTTAAAACTAATTGACGACATGTTTTTTGTTTTGTTTGTTTTCTTAAATTCCCCAATG AGAACACTTGTACGTTTTGGCATTGAGTCTTTCCATCCCTTTTCTGCGCACACTCCCCTTCTGAGCGTGT GCTGCGCAATGCCTGTCACTTCAGTAATACCACAAGCGATTTTCCTTCTTCCCTTCCACTAATTCTCAGC TCTTTAAATCAAGTTATTAACAAGCATACGTATAAAATTTGAGGAGCTTCCAAGGCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3157250 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3157250 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004340 same experiment
	EMAGE:21607 same embryo
	EMAGE:21611 same embryo
	EMAGE:21610 same embryo
	EMAGE:21612 same embryo
	EMAGE:21609 same embryo
	EMAGE:21608 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source