EMAGE mouse gene expression spatial database

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EMAGE:21610

Jun Jun oncogene ( MGI:96646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004331. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004331_01	euxassay_004331_02	euxassay_004331_03	euxassay_004331_04

euxassay_004331_05	euxassay_004331_06	euxassay_004331_07	euxassay_004331_08	euxassay_004331_09

euxassay_004331_10	euxassay_004331_11	euxassay_004331_12	euxassay_004331_13	euxassay_004331_14

euxassay_004331_15	euxassay_004331_16	euxassay_004331_17	euxassay_004331_18	euxassay_004331_19

euxassay_004331_20	euxassay_004331_21	euxassay_004331_22	euxassay_004331_23	euxassay_004331_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21610_wholemount_strong.wlz

21610_wholemount_moderate.wlz

21610_wholemount_weak.wlz

21610_wholemount_possible.wlz

21610_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21610_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 1 metacarpal

strong

regional

strong expression: see section 1 2

forelimb digit 2 metacarpal

strong

regional

strong expression: see section 1 2

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 4 3 4

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 4 3 4

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 5 3

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 4 5 6 3 4

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 5 2 3

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 4 5 6 3 4

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 5 2 3

hindlimb digit 5 metatarsal

moderate

regional

moderate expression: see section 4 5 3

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 2

telencephalon ventricular layer

moderate

regional

moderate expression: see section 9 0 1 5 6 7 8 9 0 1 2 3 weak expression: see section 2 3 4 5 6 7 8

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 9 0 5 6 7

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 2 3

ventral grey horn

strong

regional

strong expression: see section 1 2 4 5

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 9 0 weak expression: see section 1 2 5 6 7 8

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5873
Entity Detected:	Jun, Jun oncogene ( MGI:96646)
Sequence:	sense strand is shown >T5873 GCGCTCAGGCAGACAGACAGACAGACGGACGGACTTGGCCAACCCGGTCGGCCGCGGACTCCGGACTGTT CATCCGTTTGTCTTCATTTTCTCACCAACTGCTTGGATCCAGCGCCCGCGGCTCCTGCACCGGTATTTTG GGGAGCATTTGGAGAGTCCCTTCTCCCGCCTTCCACGGAGAAGAAGCTCACAAGTCCGGGCGCTGCTGAC AGCATCGAGAGCGGCTCCCGACCGCGCGAGGAAATAGGCGAGCGGCTACCGGCCAGCAACTTTCCTGACC CAGAGGACCGGTAACAAGTGGCCGGGAGCGAACTTTTGCAAATCTCTTCTGCGCCTTAAGGCTGCCACCG AGACTGTAAAGAAAAGGGAGAAGAGGAACCTATACTCATACCAGTTCGCACAGGCGGCTGAAGTTGGGCG AGCGCTAGCCGCGGCTGCCTAGCGTCCCCCTCCCCCTCACAGCGGAGGAGGGGACAGTTGTCGGAGGCCG GGCGGCAGAGCCCGATCGCGGGCTTCCACCGAGAATTCCGTGACGACTGGTCAGCACCGCCGGAGAGCCG CTGTTGCTGGGACTGGTCTGCGGGCTCCAAGGAACCGCTGCTCCCCGAGAGCGCTCCGTGAGTGACCGCG ACTTTTCAAAGCTCGGCATCGCGCGGGAGCCTACCAACGTGAGTGCTAGCGGAGTCTTAACCCTGCGCTC CCTGGAGCGAACTGGGGAGGAGGGCTCAGGGGGAAGCACTGCCGTCTGGAGCGCACGCTCCTAAACAAAC TTTGTTACAGAAGCGGGGACGCGCGGGTATCCCCCCGCTTCCCGGCGCGCTGTTGCGGCCCCGAAACTTC TGCGCACAGCCCAGGCTAACCCCGCGTGAAGTGACGGACCGTTCTATGACTGCAAAGATGGAAACGACCT TCTACGACGATGCCCTCAACGCCTCGTTCCTCCAGTCCGAGAGCGGTGCCTACGGCTACAGTAACCCTAA GATCCTAAAACAGAGCATGACCTTGAACCTGGCCGACCCGGTGGGCAGTCTGAAGCCGCACCTCCGCGCC AAGAACTCGGACCTTCTCACGTCGCCCGACGTCGGGCTGCTCAAGCTGGCGTCGCCGGAGCTGGAGCGCC TGATCATCCAGTCCAGCAATGGGCACATCACCACTACACCGACCCCCACCCAGTTCTTGTGCCCCAAGAA CGTGACCGACGAGCAGGAGGGCTTCGCCGAGGGCTTCGTGCGCGCCCTGGCTGAACTGCATAGCCAGAAC ACGCTTCCCAGTGTCACCTCCGCGGCACAGCCGGTCAGCGGGGCGGGCATGGTGGCTCCCGCGGTGGCCT CAGTAGCAGGCGCTGGCGGCGGTGGTGGCTACAGCGCCAGCCTGCACAGTGAGCCTCCGGTCTACGCCAA CCTCAGCAACTTCAACCCGGGTGCGCTGAGCAGCGGCGGTGGGGCGCCCTCCTATGGCGCGGCCGGGCTG GCCTTTCCCTCGCAGCCGCAGCAGCAGCAGCAGCCGCCTCAGCCGCCGCACCACTTGCCCCAACAGATCC CGGTGCAGCACCCGCGGCTGCAAGCCCTGAAGGAAGAGCCGCAGACCGTGCCGGAGATGCCGGGAGAGAC GCCGCCCCTGTCCCCTATCGACATGGAGTCTCAGGAGCGGATCAAGGCAGAGAGGAAGCGCATGAGGAAC CGCATTGCCGCCTCCAAGTGCCGGAAAAGGAAGCTGGAGCGGATCGCTCGGCTAGAGGAAAAAGTGAAAA CCTTGAAAGCGCAAAACTCCGAGCTGGCATCCACGGCCAACATGCTCAGGGAACAGGTGGCACAGCTTAA GCAGAAAGTCATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGCAAACGTTTTGA GAACAGACTGTCAGGGCTGAGGGGCAATGGAAGAAAAAAAATAACAGAGACAAACTTGAGAACTTGACTG GTTGCGACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156091 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156091 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004331 same experiment
	EMAGE:21607 same embryo
	EMAGE:21611 same embryo
	EMAGE:21612 same embryo
	EMAGE:21609 same embryo
	EMAGE:21608 same embryo
	EMAGE:21606 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source