EMAGE mouse gene expression spatial database

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EMAGE:29226

Capn6 calpain 6 ( MGI:1100850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006493_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7939
Entity Detected:	Capn6, calpain 6 ( MGI:1100850)
Sequence:	sense strand is shown >T7939 CTGGCTAAGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGCAGGGTTCCTGAGCTAACTCAGACCTAGTTTGATAGCAATGGGTCCTCCTCTGAAGC TCTTCAAAAACCAGAAGTACCAAGAACTGAAGCAGGAGTGCATGAAGGATGGCCGCTTTTTCTGTGACCC AACCTTCCTACCGGAGAATGATTCTCTGTTTTTCAACCGGCTGCTTCCTGGGAAGGTGGTGTGGAAGCGT CCACAGGACATTTCTGATGACCCCCACCTGATTGTGGGCAACATCAGCAACCACCAGCTGATCCAGGGCA GATTGGGGAACAAGGCAATGATCTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCACACTGGACAAAGGC AATTCCCAACCACAAGGATCAGGAATGGGATCCTCGAAAGCCAGAGAAATACGCTGGAATCTTTCACTTC CGCTTCTGGCATTTTGGAGAATGGACCGAGGTGGTGATTGATGACTTGCTTCCCACCATCAACGGAGATC TGGTCTTCTCATTCTCCACCTCCATGAATGAGTTTTGGAATGCTCTACTGGAAAAAGCGTATGCAAAGCT GCTGGGCTGTTATGAGGCTTTGGATGGTCTGACCATCACTGATATCATCATGGACTTCACTGGCACACTG GCTGAAATCATTGACATGCAGAAAGGACGATACACTGATCTTGTTGAGGAGAAGTACAAGCTGTTTGGAG AACTGTACAAAACGTTCACCAAAGGAGGTCTAATTTGCTGCTCCATTGAGTCTCCCAGCCAGGAGGAACA AGAAGTTGAAACAGACTGGGGACTACTGAAGGGTTATACCTACACCATGACTGATATTCGCAAGCTCCGT CTCGGAGAAAGACTTGTGGAAGTCTTCAGTACTGAGAAGCTGTATATGGTTCGCCTAAGGAACCCATTGG GAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCAGAGGAGTGGCAGCAACTGACTGTAACAGATCG CAAGAACCTAGGACTTGTTATGTCTGATGATGGAGAATTTTGGATGAGTCTGGAAGATTTTTGCCACAAC TTTCACAAACTGAATGTCTGCCGCAATGTGAATAATCCTGTTTTTGGCCGCAAGGAGCTGGAATCAGTGG TGGGATGTTGGACTGTGGATGATGACCCTCTGATGAACCGATCAGGAGGTTGCTATAACAACCGTGATAC CTTCTTGCAGAATCCTCAGTACATTTTCACTGTGCCCGAGGATGGCCATAAAGTCATCATGTCACTGCAA CAGAAGGACCTACGCACTTACCGCCGAATGGGAAGACCTGATAATTACATCATTGGTTTTGAGCTCTTCA AGGTGGAGATGAACCGAAGGTTCCGTCTTCACCATCTGTATATTCAGGAGCGTGCTGGGACTTCCACTTA TATCGACACCCGTACTGTGTTTCTGAGCAAGTATCTGAAGAAGGGCAGCTACGTGCTTGTTCCAACCATG TTCCAACATGGCCGTACCAGTGAATTTCTGCTGAGGATCTTCTCTGAAGTACCCGTCCAGCTCAGGGAAC TGACCTTGGACATGCCCAAGATGTCTTGCTGGAACCTGGCACGTGGCTACCCAAAGGTGGTTACCCAGAT CACTGTCCACAGTGCTGAGGGCCTGGAGAAGAAGTATGCCAATGAAACTGTCAATCCATATCTGATCATC AAATGTGGAAAGGAGGAAGTCCGTTCCCCTGTCCAGAAGAATACTGTGCATGCCATTTTTGACACGCAGG CCATTTTCTACAGAAGGACCACTGACATTCCTATTATCATCCAGGTGTGGAACAGCAGAAAATTCTGTGA TCAGTTCCTGGGGCAGGTTACTCTCGATGCTGACCCCAGCGACTGCCGTGATCTGAAATCTCTGTACCTG CGTAAGAAGGGTGGTCCTACTGCCAAAGTCAAGCAAGGTCACATCAGCTTCAAAGTTATCTCTAGCGATG ATCTCACTGAGCTCTAAGTAGTCATCATCAGAGAATCCTGACAGCTTTCCATCTTCTTTTGTCAGGCACA AGGTCTTAACTAAGACCCCACGATTACTGAAATTCCACATTTATTAATGTTTTCTCTTTTCTGATATTTC CCATACCTCCTGATCCAAGTAGCATCAGTAGCTACATAACCTATATACCTCCAGCAGCTGGACAGGGGAG GTGACAATCCCATCTGGAGATCATACACACGTTTACTGAGAAAAGATCCTTAGGGCTTCCAGGGGTGAGA TTCAAATGGGACAATAGCAAGAATGTGGACATCCTACATGTGTGTTTGCTGTTTCCACCTGCCTAACACA TTTTACCTGTAGGGCTTGTTGATGAAGGAGGACAGAGGTGATCAAGGCCAAGCTGGTCTAGCCTGACATC CCAGCTCCTAACTGACACTATGGATTTATCAGCCACATTTCACCAGTAGCCAGAGCTGACTTTTAACAGT CCCAGCCCTTACCACCACCACCACCTCTGGAAAGTGTGGTCCTGCCCTAACTCAAGTAACTCCCCCACAG TGTTTTACCTTCATGTTGAGGAAGCTTCCTAGGTGCTTAATCAAGAGCTGGAGTTCAATGCGGCTGAGAC AGTGAGAAAGGCTTGAGCTTTAGCAATTGGGAAGCCAGCTGTGTGCCATAAGAGGGAGAAGTTGAAGAAG CTGCAGTGGGAGACAAAGCCTCGGTCCCCCACCCATCCACACACACCTACACTCACACACGCGCACGCAA GCGCACACGAACTACCATTCAGTCAGTGGGCAAGAATAAGGACTAGTGAAGTATCATGCACACCAAAAGA TGGATGAATCTATTCAGTAATAGGACAGCCAGTTTGGGGTCCCTGACTACAATGTGAAACCTCTTGCTGC TGCTAGATTTACAAACAAGCCCATTGCCCTGTGCTTCCTAATATCATTGGTACTGAAAACTCTATATGGG AACCTGAGAGTCTGGGTCCCACCCCGACCCCTCAGGCTTTTCCCTCTGTTGGGAAGCTCCACTCCCCAGG GGTTTGTTACTCAGTATGTCTACAAAATTTCCTTGTGAATCAGTCATTGTATGTTCTTCCCTTCATCCTC ATCTATCAGTATTCTGCCCCTATTCAAGGCCCAGCATAAATTCCACCTCCTCCATGAAGTTCTTTCACAT CTTATCCCAAGTCCCACCTCCAACAATATTTCAGTGCTTCTGCAATGATAAAAAACTAAACATAGTTGTA GTACTTAGGCTAGTTCAGCATGCATTCATTTAAAAATACATTTTGTAGCTCGCTCTTTTTGAACTTTCTC ATTTTCTATCACATTTTGCAGTCTTTGTAACTTTTATAGTGCCTTTGCCACACTGCCTTACAAACAAAAT CACAATAAAGTTCATATTCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6517565 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6517565 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006493 same experiment
	EMAGE:29882 same embryo
	EMAGE:29871 same embryo
	EMAGE:32167 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source