EMAGE mouse gene expression spatial database

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EMAGE:29609

4632419I22Rik RIKEN cDNA 4632419I22 gene ( MGI:1921288)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017233_01	euxassay_017233_02	euxassay_017233_03	euxassay_017233_04	euxassay_017233_05

euxassay_017233_06	euxassay_017233_07	euxassay_017233_08	euxassay_017233_09	euxassay_017233_10

euxassay_017233_11	euxassay_017233_12	euxassay_017233_13	euxassay_017233_14	euxassay_017233_15

euxassay_017233_16	euxassay_017233_17	euxassay_017233_18	euxassay_017233_19	euxassay_017233_20

euxassay_017233_21	euxassay_017233_22	euxassay_017233_23	euxassay_017233_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8596
Entity Detected:	4632419I22Rik, RIKEN cDNA 4632419I22 gene ( MGI:1921288)
Sequence:	sense strand is shown >T8596 CCCAAACCCCACCCCGCGAAAAAGACTGGGACAAGTTCCGCACATTTGCTTGAAGGAAAAAAAAAAATTC TTGTACTGTGAGACTTTAAGAAGCACAAAATGGGCGTTAGACGGGTCTTACATTTGGTGCATTGACCGGG AATCGTCCTGCTGGCGGGTCCAGTGACAGGAGACCGGAGGATTGTTCGAGCAGGTTAAAAGGCCTGCAGC TGGTGCAGAGAGAAGTGTGATCACAGCTGGCAGCTGCATATGAGCCTGATACTCCTAAGACATCGCATCA GTTCGAGGTTGGAGATTCATTCTACGTGAGGCAACATCGTGCCCAGAGGCTCGAGCCCCATTGGAAAGGA TCCTATCTAGTACTGCTAACTACTCCAACGGCTGTCAAAGTGGATGGAATTGCTGCCTGGATACATGCCT CACATGTGAAGCCAGCCCCCTTAGCTGAGACCAGAATTCCAGGAAACCACCCTGTCTGGAAGGCCCAGGC TACGGAGAACCCTCTGAAAGTGAAGATCGTCAAAGGGTCCAAAGATGCAAGCCCTTCCCCTGGTACTGTA TCTTAGTTTCTTCTCCTTATATTCTGCAGACCTTGGTCCCCACCAGCCACGCAACCTAACGTGGCAGGTG ATATCAGGGGCTGGAGACACTATTTGGAGTCTATCCAAGGTTACCACTCCATCTACCTGGTGGCCTGACC TTTTCCCTGACATCTGTAAGTTGGCTATAGGAGCACCTCCTGGATGGGACTTGGAGGGGTACTCTGACAT TCAGAGGGCACCTTCAACACCCCCTCCGTATGTAGAAAAACATCCAAGAGACCCATGGGGTGGTTGCTCT AACCAAAGGGATAGAAGCATGCTTCGAATCCACCCCTTCTATGTCTGCCCCGGGCCCCACCGAAGTCAGT CCCTCAATCCAAAATGCGGAGGTAAGACTGACTTCTTCTGCAAGAGCTGGGGTTGTGAGACTTCAGGTGA AGTCTACTGGAAGCCCTCCTCGAGCTGGGACTATATTAAAGTAAGAGCCAACTATTCCCTAGAGTCTTAC GTACCTGGAGGATTTGACCTAGACGAGTGTACCGACTGGTGCCATCCACTCCGTGTCACCTTCACTGAAC CAGGGAAAAGAGCTCTAGAATGGACAAAAGGATACACCTGGGGTCTTAGGCTTTATAAGGAAAGATATGA TGAGGGATTATTGTTCACTATCAGATTAAAAATAGAGACCCCCTACAATCCTTTAGGCCCCAACAAAGTC TTGGCCCCAGATGAGATAAATCCCAGGCCAACACCCCCCTCTAGTTCCAGGCCAACACCCCCACCCAATT CTCGTGGTACTGTGGCTCCTTCAAAAAGGATCACGGAGACTGTTTCCCTTTAATCGCCTGGGCAATCTCA TTTAACTCATCTAGTTCAAGAAGCTTTCAGAATTATCAACAGCACCAACCCTGAAGCCACTAAATCATGT TGACTCTGTTTATGATGTAGCTCCTCCTTACTATGAAGGCATGGTATTTATAAAAATGCTAAAATCACTC TGATGCCACAACATACCAGTGGCAGCAGCAGAGCGCCAGACTTAACCTTGTCAGCTATAACTGGGCAGGG ACTGTGTGTGGGAAAAGTTCAGTCTTGTTGGGGGTGGGGCTGGGAGTGGCAGGGGCTGCTATGGGATCAT CTGCACTGGCCTTGCAGACCCAGAGTTACCAAAGTCTGATGGCTGCCATAGATGCTGATATTGTAGAGAT GGAAAACTCCATCTCTAAGCTCCAGGAGTCTCTGACCTTGCTGTCAGAAGTGGTCCTCCAAAATAGGAGA GGCTTGGATCTCCTGTTCCTCCAACAAGATCTGTGTGCAGCCCTAAAAGAAGAATGCTGCTTCTACATAG ACCACTCTGGAGTGGTAAAAGATTCGATGGCCAAAGTGAGAAAGGGGCTGGCGAAAAGAAAGAGAGAAAG AAAACAGAACCAGGGGTGGTTTAATCTTGGTTTAACTCTTTCCCCGTGGCTTCACGACCCTGATTTCTAT CCTTCTGGGACCATTGCTAATCCTGATGTTGCTTTTAACTTTTGTTCCCTGCATTTTAAATTGCTTGCTA GCTTTTATTAGAGAACACATAAGCACAGTTCAGATTATAATGCTGAGACAACAGTATCAAGAGTTAAGTC AGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCCTGAGTTCAAATC CCAGCAACCACATGGTGGCTCACAATCATCCGTAATGAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGA CAGCTACAGTGTACTTACATATAATAAATAAATGAAGAGTTAAGTCAGAATGAGCTATAATTTCATGACT GAAATACAAGAAAAAGGGGGGAATGTGAAAGTTTAAGAAGCACAAAATGGCTGCTAGAAGGAGGATCTTG CCAGAAATAAGACTTTGGGTACAGAAGGATTATGACTAATTCCTCTTCCCAGAATGTCCCAGAAAAACAG GAAGGCCTTTACTGCAGAGCAGGGTGGGCCAGGTGCAGATAATTCAGTTCCCCTTTGAAGATAACAAAGA CCTCACCCCACTTAGAACAACCAATGCTGAGAAAGGTCGATACAGACTACTAATAAGCTTTAACCAACTC ATACTGTTGCTGAAGTCTGCCCCCAAGAACTGTATAAAAGATGCTTGAAATGTTCAGGGTAGCCTCTGCC CTAATTGCAGGATGGCCCCAATGCGCTGGATCATTAAACCTCTTGCTTGTTGCAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716200 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017233 same experiment
	EMAGE:29571 same embryo
	EMAGE:29597 same embryo
	EMAGE:29563 same embryo
	EMAGE:29589 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source