EMAGE mouse gene expression spatial database

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EMAGE:29789

Cebpg CCAAT/enhancer binding protein (C/EBP), gamma ( MGI:104982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006937_01	euxassay_006937_02	euxassay_006937_03	euxassay_006937_04	euxassay_006937_05

euxassay_006937_06	euxassay_006937_07	euxassay_006937_08	euxassay_006937_09	euxassay_006937_10

euxassay_006937_11	euxassay_006937_12	euxassay_006937_13	euxassay_006937_14	euxassay_006937_15

euxassay_006937_16	euxassay_006937_17	euxassay_006937_18	euxassay_006937_19	euxassay_006937_20

euxassay_006937_21	euxassay_006937_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6791
Entity Detected:	Cebpg, CCAAT/enhancer binding protein (C/EBP), gamma ( MGI:104982)
Sequence:	sense strand is shown >T6791 CGTAACCGTCGCTCCTCCTCGCCGACTCGCGGGCTGCGAGGCCTGGGTCGGGTCGGGTCGGGCCGCGCCG CGCGGGGCCGCTCGGAGTGGAGGCCGTCTGGGGGCGGGCGGGCCGGCCGGAGGCGCAGGTACATGTGAAG ATTTTTTGGCAGTTGAGTGTGGCCTCCTCGGATCACATTGCTCTGATTTCTACCTATTCTGTGTTGGCAA AGGAACGTGCCCAAATGAGCAAGCTGTCGCAGCCAGCCACTACTCCAGGAGTGAATGGAATAAGTGTCAT TCATACTCAGGCACATGCCAGCGGCTTACAGCAGGTTCCTCAGCTGGTGCCCGCTGGGCCTGGGGGAGGG GGCAAGGCTGTGCCTCCAAGCAAGCAAAGCAAAAAGAGCTCACCCATGGATCGGAATAGTGACGAATACC GCCAGCGCAGAGAGCGGAACAATATGGCGGTGAAAAAAAGCCGGTTAAAAAGCAAGCAGAAAGCTCAAGA TACACTGCAAAGAGTAAACCAGCTCAAGGAAGAGAATGAACGGTTGGAAGCCAAAATTAAGTTGCTGACA AAGGAATTAAGTGTACTGAAAGATTTGTTTCTTGAGCATGCGCACAGCCTCGCAGACAACGTGCAGCCCA TCAGCACGGAAACTACAGCGACAAATTCTGATAACCCAGGGCAGTAGATCTCCTTCCAGGCCCAGGGCTT GTGACTTGAACATGAGAGGTGTGACCACCCTGCACCCCTTGCTTCAGTGGCTGAACTCGGTCTCCTTCCA TTGGAGGTTGTTTTCTAGGCTCAACACTGAACAGCTGATTAGCCATGTAATTTATCTGGTCTTAAATGAT AATGGATTTTTGAAGCACTAAAAGGATTTAGGGTTTATCGTTAAAACAAAATTCCTGGACTTTAATATTC TTAATAAATCCTCACTTCCCCAGAAATGGCTCTTTTGTAGGAATGGGACAATGGAAGGTCATGTAGAAGG TACTGGGTTCTAATGAGAGAATACCTTGGATTAAGAAAAGCTGAATTCTAGCCGGGCGGTGGTGGCACAC GCCTTTAATCCCAGCACTTAGGAGACAGAGGCAGGCGGATTTCTGAGTTCGTTGCCAGCCTGGTCTACAA AGTGAGTCCTAGGTCAGCCAGGGCTATACAGAAAAACCCTGTCTCGAAAAACAAAAACAAATAAACAAAA AAAACAAAACAAACAAACAAAAAGAAAAGCTGAATTCCATCTGGGTTCCTCAGTTTGTGATTTTATTCTA AATTGTGTATGGTAAATTCTGGGGCTACAAAAACCAGTCCTAATAATTTGGCCCTATTTTATAGATGTAT AAACCTGTGTTAGCATTGTCAGCATTGTTTTTTCCAACATACATATTTTTAAAAATGACTTTAGGTGAAG TAAAGAAACCCCATCTGTCATGTTGAGGAGGTGGTTAAAATACAAAGTGTGTTTAGGAAGGAAGAAGTGG TCTTGGGCTTTTTTTTTTTTCCCTTTTGTTTGTTTTAAAGGGCATAGAGTGCGATTGAACTTTGAGGGGC CTTCTGCTTATTAGATAAGCATGGTCTCTGTCCTAAAAAACAGCATCTACTGTGTACTGACATTTTAGTT TCTGTGGACGCAAGTAAATGCAGCATTTGGTTTGGGGGAGAAACCATTTTTTGTCAGCCAAGTTAGTAAA TAAAATGCCTTGGCTTGATGATACAGACCATGATTTACAATACTACTAATTCATTTGGTTTCTCCTGTGT TTGTTCAAAATAACTGCCAATGGGGCCATGATTTTTAAAGGTAAAGTGTTCGTGTGTGTGTGCTTGTGTG AAGAGGCACCTTTTGTTGTTTGATCTCAACTGAACAATTATTAGAAAAATGAATCAACTTTATGCCATCT CTCAAAAGACTAGTCAAAGAAAACTTGAAAGTATGAGGTTTGAATCTTTAATTTATTTCCTAAATTTTTA AATTTTTATTTCTTTGAGGAATCTTTTTCTAGTTTAGTGGGCTGTTCTTCCAGACCTCATGTGTTATTGC TTCCATTAGTATGCTTTTGTGTTCTGTATAACTTATTTTAGAGAGAAAATGCTGATAAAACTCAGGATAT TGACTCTTGTTGTGAAACTGAAAAGCTGGGTGTCTGTAGGGTCTGCTCTTATCAGTGTTGTATTTAGATC GTCTTCTTGGTAACTCATAGTTTGTGCACAGCTCCAGTGTCAGTTTGTTAGTTCCTGAGACGGTAGCTAA ACATGGGTCTTGTGTGATCAAAGTTTGGGTTCTGGGAATCTTGTTTGAATGTGGTCATTTCTGGCCCTGT GTTCTACTAGGACTTGGTAACCCCAGTGTCGCTCAGGTTGCTCCCTAGTATGGCAGGAAGTTGCTGACTG TTCGGGACAGAAGCTTTCAGGGCCCTGGAGCTCAGCAGAGCTGAAGACAGGAGCCGACACTGTTCTTTGG GAGCCAAGAAATGAGTGGGGTTTTAAAAATTAGGGCTCAGTACCATTTTACCTGGGTTGGAATACCATCT ACTCCTAAAGATTGAGAGATGGTTTGGATAACTAACAAGGACTCACCCATATAATCCTAATCTTCATCAG TGGATTGATAATTACGTAATGAATTTAATTGGTGACACAGTTAGGTCCCACTTGAACATGTATGAGGCTA GGCAGACTTCCGGAAACTCTTACTTCTAAACACCAGCTTTCACAAATGGATGTTCTTGTGGAATTTGTGG TTACCAGTGCTAGGACTTTAAAAAGCTGTAGGGAGGTCAGTGCTCAAGGTATCAAATCAGAACATACAAA TGAGGTCCATCTTTGCTCTTACCTTTGTAAAAGAGATTGTTAGCACAAGCAAGTAGTTCCATATTGAACA CAAATGTTTGGACTCAAATGTTCTTACTCAAGTGGGAATGATGTTTAATTTGATAGATTTCTCTGTAAAG TTGGGGGAAACTAGGTTTGGGGAATGGGTAAACTTAAGGCTTTGGAAACTGTAGAGTGACTTTTGAAAGT CTTGTTACCGGGGGACCTTTAGAGTTTGATCTGCCTTGTGGACAGGTTGGTGGCTTAGGAATCAAACTGT AGTGTGAATTAGCCTTGTCTCTGTCTCCTGGGATGGCTGCCCTTTGACGAGTTCTGGGCATAGAGGGGGA ATCAGAGCCACCCTCTCTGTCTAGCCTTTTGGGTCACAGGTGACTTTGGGATCTCAGTAAGGATTTAACT AAAATATGACTGGGTGTTTGAAATTGGGTCATGAGGCCATTCAGTTTTTACGGTTCTCAATTTATGAAAC TAGAAACATGTACAGTTTGATAAGAAAGGATGCAGCTGCTTTTGTTTCTTAACCCTCTCACCCCTTTCCA GGGAAGCTTCGTGCCTGGACAACCCATGGAGCTTGTAGAGTGCTCCTGATGCCTTAGAAATAGGAAAAGA CGCCTGTTTGTGTGACAGGCTGGGGAAGATTCCATTCCACAGCTAATCAGATCTGCCTCAGGAAAAATAC TAACTTGTTCTATCTGTTCCTGGCCTTCAGTTTGTGAGATTACTCTATTTTTTTAAAATATAATTTTATT TCTTTCAACGAATTTAAAAATAAAAAACACCTTTTGGAACAACGACTTTTTCTTCGTGGGTTTCTTTATC TTGGACATGAGCAAGAAGTTCATTTGTAATTGGCCCCAAAGAGCCTGCAAGGTGCCTCTTGCTGTTGTCC TGGGCTGGAGTGCATGGACAGCGGGGTACTTTGTGCAAGTTTGGATTTCCCTGAGTTGACAGGGAAGGAG TTGTCTCTAAACTTGGAATGCAGCTTCAGTTGATTGTAGTTGTTTGGTCTAACTTAAAACTGCATCCCAG TGTAGGGGGCAGGATGAAAGGGCTGCCTGTGTCCTGGTGGGTCCTAGCCTATGGTTTCCATTTGAGGTGT ATCCCCAACACTACATTTTCTAGTATGAATACCATCTTGACTTACACAGGGTATATGCCTGGATATGGAA GTGTACTGGCTGATTTCAGAAATAAAACAAGACTCTAAGAAACAGAATTGTGTGTTGGGGGTGGGGGGTG GGGCAGGAAGAGGCCATTTAGACGCTTTACTTTGGTCCTTTTAGTCTTTTCGTGTTTGGGGAAATAAGTT TCTAATTGTTGTGTGGTTGTGAAAATTGTGTGGCTGATTATGGTGGTATACACCTGTAACCCCAGTATTA CAGAGATGAAGGTTGAGTTTAAGGCCAATTTGAGTTTGAGTACCATTGAGACCCTGTCTTAAAAATGAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709074 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709074 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006937 same experiment
	EMAGE:31928 same embryo
	EMAGE:29792 same embryo
	EMAGE:31909 same embryo
	EMAGE:32202 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source