EMAGE mouse gene expression spatial database

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EMAGE:30241

Ppil4 peptidylprolyl isomerase (cyclophilin)-like 4 ( MGI:1914668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007901_01	euxassay_007901_02	euxassay_007901_03	euxassay_007901_04	euxassay_007901_05

euxassay_007901_06	euxassay_007901_07	euxassay_007901_08	euxassay_007901_09	euxassay_007901_10

euxassay_007901_11	euxassay_007901_12	euxassay_007901_13	euxassay_007901_14	euxassay_007901_15

euxassay_007901_16	euxassay_007901_17	euxassay_007901_18	euxassay_007901_19	euxassay_007901_20

euxassay_007901_21	euxassay_007901_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9607
Entity Detected:	Ppil4, peptidylprolyl isomerase (cyclophilin)-like 4 ( MGI:1914668)
Sequence:	sense strand is shown >T9607 CTGCGGAGGAGCCGGCGCCATGGCGGTTCTCTTGGAGACCACTCTGGGCGACGTGGTCATCGACTTGTAC ACTGAAGAGCGTCCCCGGGCTTGCTTGAATTTTTTGAAGCTGTGCAAAATAAAATATTACAACTATTGCC TCATACACAATGTACAGAGGGATTTTATCATACAAACAGGAGATCCCACGGGGACTGGCCGCGGAGGAGA GTCTATATTTGGACAACTGTATGGTGATCAAGCAAGCTTTTTTGAAGCAGAAAAAGTACCAAGGATAAAG CACAAGAAGAAAGGCACTGTGTCCATGGTGAACAACGGCAGTGACCAGCACGGGTCTCAGTTTCTTATCA CTACAGGAGAAAACTTAGATTACCTTGACGGTGTTCATACAGTGTTTGGTGAAGTGACAGAAGGCATGGA CATAGTTAAGAAAATCAATGAGACCTTTGTGGACAAGGATTTTGTACCATATCAAGATATAAGGATAAAT CATACAGTGATTTTAGATGATCCATTTGATGACCCTCCTGATTTATTAATTCCTGATCGATCACCCGAGC CTACAAAGGAACAATTAGATAGTGGTCGAATAGGAGCAGATGAAGAAATTGATGACTTCAAAGGAAGATC AGCTGAAGAAGTAGAAGAAATAAAGGCAGAAAAAGAGGCTAAAACTCAAGCTATTCTCTTGGAGATGGTG GGAGACTTACCTGATGCAGATATTAAACCTCCAGAAAATGTGCTGTTTGTGTGTAAATTGAATCCAGTGA CCACAGATGAGGACTTGGAGATAATATTCTCAAGATTTGGGCCAATAAGAAGTTGTGAAGTTATCCGGGA TTGGAAAACAGGAGAGTCCCTCTGTTATGCTTTTATTGAATTTGAAAAGGAAGAAGACTGTGAGAAAGCA TTCTTCAAAATGGATAATGTGCTTATAGATGACAGGAGGATACATGTGGATTTCAGCCAGTCTGTTGCAA AGGTTAAATGGAAAGGAAAAGGTGGGAAATATACCAAGAGTGATTTCAAGGAGTATGAAAAGGAACAGGA TAAACCAGCCAATTTGGTTCTGAAAGAAAAAGTAAAGCCCAAACAGGATGCAAAATATGATCTTATACTA GATGAGCAGGGAGAAGACTCCAAATCAAGTCACTCACACACAAGTAAAAAGCACAAAAAGAAAACACGCC ACTGCTCCGAAGAAAAAGAAGATGAAGAGTACATGCCAATCAAAAATCCTAATCAGGATATCTACAGGGA AATGGGTTTTGGTCATTATGAAGAAGAAGAAAGCTGTTGGGAGAAACAGAAGAATGAAAAGAGAGACCGA CGTCAGAACCGCAGTCGTAGCCGGTCTCGAGAAAGGGATGGTCACTATAGCAACAGCCACAAACCCAAGT ACCAGACAGAACCTTATGAGAGGGAAAGAAGCAGAAAGAGGGACAGAAGCAGGAGTCCAAAGAAATCCAA AGCTAAAGAAAAATCCAAGTACAGATGAGCAGAGGAGGTGGACAATTGTAGCAGGTCTGCTCCGGCCTGA GCTGGGGCACGCAGACGCACTCAGTGCCTGCCAGCACAGCTGACTGCGGTTTCGATGTTAGCGTTGTTGC CTTTCAGGTTACACTGATGGTGTGGGGCTCTGGAAGAGAGGGAGGCATTTTGTGTATACTTTTTATACAA ATTTTATTGTTGTGCATACATGGTAACGTTCATATTTGACATCTTTCATAGTTTCAGTCTTTTAAGAATG AATCATTTCATATCACAGAAGTCCATAAACTTGTATGTAAAGCATAAAACAGAAAATGTACCTGGCTGCA GCGCAAGAGGTAAGAGTGTCACTCACTATGAAAGCTCCATATATCCCTTCTCCTTGCAGGAATTATTCTG AGTTGTGTTTACCCTGCTGTTAATTTTGTATGTTTTTGAATTTTATACAAATGGCACAATGTATATTTTC CTTCTGTGACTTTCTTTTTTTAAGAGTCCATTTATTTTTATTGTTACATAGTTGTGAATGTACTACGCTT GATCTGTTGATGGACTTTTAGGTTGTTTGTTAGTTTTTGCTATCTGAGGAATGCCACTGTGAACATTTCA ACTGAGCATGGCACTTTCTGGCCATGTGGTTTGAGTTTTCAATGCATTTATAGTTATGGTTGCTGTAATT TACCTGGTATGTGAATTGTATGGGGTCAGGGGTAGCCACAAGAGTAGTTAGTCTACCATACAGCTCAGAG CACATTTGCTCCATTATTGTACATAGTTTTGACTACCAATAGAAAAGGCTTAACTATATCAGGAGCTAGC ATCTTTTCTAGGGAGATGGCTCAGTGGGTACAGTGTTTCCTCATCAGCACAGGACTGAGTTCAGATCCTC ACCACATACCCAGTGCATGCGACTAATAATCCAGTACTGGGGAGACAGGAGCGGGGGAAGCTCCAGGCAG TTCGAGTGACCGAGGAAGACACTCGGCACTGACCTCTTGCCTCCACATACTGTACAAACTCGTGCGTGCA GGCACACACACACCCGAATACACAGCTGTTCTGCATACAGAACGGTAGGTGTTGCTCACACAGATGAACC TAAAACATGGTCTAGAAGAATGAGCAGGGACAGGCTGGAAGCAACGAGAAGTCATCCACTGACTGCGGAG ACTCGATTTAAGAGCTCTGGAGTATTGAAATAGGAAAATGTATGCACCAAGCGTTTCAAGGAAAACATAT CCTTGATTCCAAATGATAAGAAACCGTTTTATTTTTCCTATGTATTTGACAGTAACTATATGCTTATGGG AGTGTAATATGTTTGCTATATGTTAATATTCTGGAACAAGTAAATCCATTTGCAGACAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30661438 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30661438 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007901 same experiment
	EMAGE:31637 same embryo
	EMAGE:30239 same embryo
	EMAGE:30173 same embryo
	EMAGE:29621 same embryo
	EMAGE:30238 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source