EMAGE mouse gene expression spatial database

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EMAGE:30334

Phc1 polyhomeotic-like 1 (Drosophila) ( MGI:103248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004014_01	euxassay_004014_02	euxassay_004014_03	euxassay_004014_04	euxassay_004014_05

euxassay_004014_06	euxassay_004014_07	euxassay_004014_08	euxassay_004014_09	euxassay_004014_10

euxassay_004014_11	euxassay_004014_12	euxassay_004014_13	euxassay_004014_14	euxassay_004014_15

euxassay_004014_16	euxassay_004014_17	euxassay_004014_18	euxassay_004014_19	euxassay_004014_20

euxassay_004014_21	euxassay_004014_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4453
Entity Detected:	Phc1, polyhomeotic-like 1 (Drosophila) ( MGI:103248)
Sequence:	sense strand is shown >T4453 ACGCCCCCCGCGAGGACTCGGGTGCGGAGCCGGGGGCGAAGGGCTTGAGTCAGAGCGCCATTCTTGGGGA CCCTGGAACGCTATCATGGAAACGGAGAGTGAGCAGAACTCTAGTTCGACCAATGGGAGTTCCAGTTCAG GGGCCAGCTCTCGGCCCCAGATAGCACAGATGTCCCTGTATGAACGGCAAGCAGTGCAGGCTCTGCAAGC ACTGCAGCGGCAACCTAATGCGGCTCAATATTTCCACCAGTTCATGCTCCAGCAACAGCTCAGCAATGCC CAACTGCACAGCCTGGCAGCTGTCCAGCAGGCCACGATTGCTGCCAGTCGACAGGCTAGCTCCCCAAACA GCAGCACTGCACAGCAGCAGACCGCTACCACCCAGGCCTCGATGAATCTCGCGACCACGTCAGCTGCTCA GCTCATCAGCCGATCGCAGAGTGTGAGCTCCCCCAGTGCTACCACCCTGACGCAGTCGGTGCTGCTGGGG AACACCACTTCCCCACCTCTCAACCAGTCCCAGGCCCAGATGTATCTGCGGCCACAGCTGGGCAACCTAT TGCAGGTTAACCGGACCTTGGGCCGGAATGTGCCTCTTGCCTCCCAGCTCATCCTGATGCCTAATGGGGC AGTGGCTGCAGTCCAGCAGGAGGTGCCACCCGCTCAGTCTCCTGGAGTTCATGCAGATGCAGATCAGGTG CAGAACTTGGCAGTGAGGAACCAACAGGCTTCAGCTCAAGGACCCCAAATGCCAGGCTCCACTCAGAAGG CCATCCCTCCTGGAGCTTCTCCTGTTTCTGGCCTCTCCCAGACTTCTAGCCAGGCCCTAGCCGTGGCACA GGCTTCTTCTGGGGCCTCAGGCCAGTCACTCAACCTTAGTCAAGCCGGTGGAGGCAGTGGGAATAGCCTC CCAGGCTCCATGGGTCCAGGTGGAGGTGGCCAGGCGCCTGGGGGTTTAGGTCAGTTGCCTTCTTCAGGAT TGACTGGTGGAAGCTGTCCCCGGAAGGGCACAGGAGTGGTGCAGCCCTTACCTGCAGCTCAAACAGTGAC TGTGAGCCAAGGAAGCCAAACAGAAGCAGAAAGTGCAGCAGCCAAGAAGGCAGAAGCAGATGGGAGTGGC CAGCAGAGTGTGGGCATGAACCTGACTCGGACAGCCACACCTGCCCCCAGCCAGACGCTTATTAGCTCAG CCACATACACACAGATCCAGCCCCATTCACTGATTCAGCAACAGCAGCAGATCCACCTCCAGCAGAAGCA AGTGGTGATCCAGCAGCAGATTGCCATCCACCACCAGCAACAGTTCCAACACCGCCAGTCCCAGCTGCTT CACACAGCCACACACCTCCAGTTGGCCCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAACAGC AACAGCAGCAGCAGCAGCAGCAGCAGCAAGGAACAACCCTCACTGCCCCTCAGCCACCCCAGGTCCCACC TACTCAGCAGGTCCCACCTTCCCAATCACAACAGCAAGCCCAGACTCTGGTGGTCCAACCCATGCTTCAG TCTTCACCCCTGACTCTTCCACCTGAACCAACCTCTAAACCACCCATCCCCATTCAGTCCAAACCTCCTG TAGCGCCTATCAAACCTCCTCAACTAGGAGCTGCTAAGATGTCAGCTACACAGCAGCCACCACCACATAT CCCCGTGCAAGTTGTCGGTACCCGGCAGCCAGGTTCAGCCCAAGCACAGGCTTTGGGATTAGCACAGCTG GCTGCTGCTGTACCTACTCCCCGTGGAATAACAGGAGCAGTCCAGCCTGGCCAGGCTCATCTGGCCTCCT CGCCACCTTCATCACAGGCGGCTCCTGGTGCACTTCAAGAATGTCCTCCTGCATTGGCTGCTGGGATGAC CCTTGCTCCTGTGCAGGGGACAGCACATGTGGTAAAGGGTGGGCCTACAGCCTCCTCTCCTGTTGTGGCC CAGGTCCCTGCTGCTTTCTACATGCAGTCGGTGCACCTGCCGGGTAAAGCCCAGACCTTGGCTGTGAAAC GCAAAGCTGAGTCTGAGGAGGAGAGAGATGACCTCTCCGCATTGGCCTCTGTGCTTCCTACAAAGGCCTC CCCAGCAGCAGAGAGCCCAAAGGTAATAGAGGAGAAGAATAGTCTTGGAGAGAAAGCTGAACCAGTGGCC AGTTTGAATGCTAATCCTCCAAACAGTGATCTAGTAGCCTTGGCCCCTACCCCATCAGCACCACCTCCTA CTCTGGCCTTGGTGTCCAGACAGATGGGTGACTCAAAACCCCCACAGGCCATTGTGAAGCCCCAGATTCT CACACACATCATTGAAGGCTTTGTTATCCAGGAAGGAGCAGAGCCTTTTCCGGTGGGATGTTCTCAGTTC CTGAAAGAAACCGAGAAGCCACTGCAGGCTGGCCTTCCGACCGGGCTGAATGAGAGTCAGCCAAGTGGCC CTTTGGGGGGAGACAGCCCCTCTGTAGAGTTAGAGAAGAAGGCAAATCTCCTCAAGTGTGAGTACTGTGG CAAGTACGCCCCAGCAGAGCAGTTTCGAGGCTCTAAGAGGTTCTGCTCCATGACGTGTGCAAAGAGGTAC AATGTGAGCTGCAGCCACCAGTTCCGCTTGAAGAGGAAAAAAATGAAAGAGTTTCAGGAAGCCAGCTATG CACGTGTTCGGAGGCGTGGACCCCGCCGCAGCTCCTCTGATATTGCTCGTGCTAAGATCCAGGGCAAGCG CCATCGGGGTCAAGAGGACTCTAGCCGGGGATCAGATAATTCCAGCTATGATGAAGCACTCTCTCCAACA TCTCCTGGGCCGCTGTCAGTAAGAGCCGGGCACGGAGAACGTGATCTAGGAAACACCATTACAACCCCAT CCACGCCAGAGTTACAGGGCATCAATCCCGTGTTCTTGTCCAGTAATCCTAGCCAGTGGAGCGTCGAGGA GGTCTACGAGTTTATTGCTTCTCTGCAAGGCTGCCAAGAGATTGCAGAAGAGTTTCGTTCCCAGGAGATT GATGGACAGGCCCTTTTATTACTTAAAGAAGAACATCTGATGAGTGCCATGAACATCAAATTAGGTCCTG CTCTCAAGATCTGTGCCAAGATCAATGTCCTCAAGGAGACCTAAGTGACCCTCTGCACAATTCAGACTAA GGCAGAAGGCCTGGCTGTTCAGGTTATAACCTGGAAGAGCTGTTCCTTTTGTGTGACCTGTGGGGTTTGG AGGCAGAACTCTTAAGGATTGGTTGCTGGTGCTACATGGTGACAGCTTTGATACCTTCTCTGGGTTTCAC TTTAACAATAAAAACCTTCTCACTATTCACACCTACCCTAATCCAGTTTTTAAACAGGCAGACTAGAGAC TAGGACTGCTTAGCCTTCCTTATCCTGGAGTGGAGGGACTAGCCAGGGTTCACTTCTCCAAGGAGGAATG GATGGTAAGGCAAGGAAATGGATGCCATGTAGGTTTGCCTCCCTATGGGAGAGGTCAGCTTTCCTAGCTT ATGACAGGAGCAGCAAACCTGGTACTCTGCTGGAGTCTCACTGTGGCTGTAGCTCAGTTTTCTGTTTCAT TTTCTCCCTTAATACTGAATATTTCTTCTGCATTCATGGCAGGGTCACCAGCCATCATAGACACTTGGTT GGTATCGGTTTTGCTGCAGGGACGAAGTGTTTGAGTGACACATGTGGCTGTTGCCATTCTTTCTGCACCC CAGCTCCCAACTGTTCCCACCTTCAACCCTCTTTCCCATTCCAACGCTGCTCTGTCCTATAGCTTCACAA AACAGGAGCGTGTGGGGCTGAGGTCAGGACTGTACCTTGGGCACTATTCCTTATACAAAATATTAAATAT TTTTTTTCCTCAGTAAAAGGATGAAAGTTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935713 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935713 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004014 same experiment
	EMAGE:30943 same embryo
	EMAGE:30031 same embryo
	EMAGE:30947 same embryo
	EMAGE:30897 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source