EMAGE mouse gene expression spatial database

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EMAGE:30593

Rcan1 regulator of calcineurin 1 ( MGI:1890564)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7469
Entity Detected:	Rcan1, regulator of calcineurin 1 ( MGI:1890564)
Sequence:	sense strand is shown >T7469 TGCAAAGGAACCTCCAGCTTGGGCTTGACTGAGAGAGCGAGTCGTTCGTTAAGCGTCTGCCCCGTGAAAA AGCAGAATGCATTTTAGGGACTTTAGCTACAATTTTAGCTCCCTGATTGCTTGTGTGGCAAACGATGATG TCTTCAGCGAAAGTGAGACCAGGGCCAAATTTGAATCCCTCTTCAGAACATATGACAAGGACACCACCTT CCAGTATTTTAAGAGCTTCAAACGTGTCCGGATAAACTTCAGCAACCCCTTATCTGCAGCCGATGCCAGG CTGCGGCTGCACAAGACCGAGTTCCTGGGGAAGGAAATGAAGTTGTATTTTGCTCAGACTTTACACATAG GAAGTTCACACCTGGCTCCGCCCAATCCCGACAAACAGTTCCTCATCTCCCCTCCGGCCTCTCCTCCCGT TGGCTGGAAACAAGTAGAAGATGCCACCCCCGTCATAAATTACGATCTTTTATATGCCATCTCCAAGCTG GGGCCAGGAGAGAAGTATGAACTGCATGCAGCGACAGACACCACTCCCAGTGTGGTGGTCCACGTGTGTG AGAGTGACCAAGAGAATGAGGAGGAAGAGGAAGAGATGGAGAGAATGAAGAGACCCAAGCCCAAAATCAT CCAGACACGGAGACCGGAGTACACACCCATCCACCTCAGCTGAACCGGCGCGCAGACTGAGACAGCGCTC TGAATCCCACGTGTGGGAAGGAATCTTTTACTGTGCAGGTGGCTGGCCGTGGCTTTGGAGGGAGCGGCCG TGACCACTGTGGCAGAAATTCCAGTTCATGATGCTCAGAAGAGAGTCAAGGCCTCCTCCCCTTGTTCTGA TGCTGCCCCTCAGTCCATGTCCACGGCACCTGGGACTGTCTTGGGCCATTCACTGAATGTGTGGCAGTCA CACAAGGACACTGGGGACATCCTGAGAAACTGATAGTTCTTGTAATTGCTCATTTCTAGGTTCTGTTTTT GGCAAGGACAGGTTGACTGGTGGCCCAGGGAGAGATTTCTGTTTCCAACCAGAGTTCAGGGTGCAGCCCC CCCACTGGTAGGCAGAGGCACACCCCCCAAAGCCACCAAGTGTCCAGTGCAGAAAGGTGGTGGGACAACC CCCGAACCGTGTGGAATTGTCCTTCTCGTTCCTTCCCCATGGTCTGACGTTCATGCATGTGTATTACTGG GTCCCAGGACCAGCCTTTGTTCACGTAGGAAGTTACGCTGTTTTCCATCTTGGGAAGCCAGGAAAGTTCT GGAACACTCTTACCACGTGTCCTCGTTTTCTCTGCACATGGGGTCGAGGCTGGAGACATCCACACGTCAC CCTGCCTGGTGCATTGTGTCACTTGTGCTTCCCTGCAAAAGAAGTTGATCTTGAGTCCCCAAGTAGAACT TAGCCTAACCTGTGGGAGAGCAGCAGGGTTGCTGCCAGCCGTCTGTCTGGGCTGGGAAGGTTTGGTGTGG GTGCTTGCGTTCAGCACCGGTCTCCGTTGTTTCCTTCATGTAGTGGTTTGTGTGTGAATTTCATCCCAGA CAGTCTTTGGGAACTGTTGGTTGAGACAGTTTTTCACCCCCATTGAAACGTGAAGATAAACTTGTAAATA CAGCCAAGAGCATATCTTAGTACCTGTCTGTACCTGGGTGGAAGTAGAGGGCAGAGGGAGTTTCAAGCTA CCTGTGACTTGTGTGTGGTAGAGGTTACTCTCATGTGTTTAATGGGGTGAATCAGAGCATGTGGAGTCCG TCGTAGCCATGTGTTCCTAGAGGGACACAGTGCCTTTCCCCTTTCATCCAGATGACCACGACACTCGGTG AACATTTGAGAATTTCTGCTTTCCCAGTCATCGTGTGTGTGTCTGGTAAATAGGTATTCTATTTTGGTCT TACCATTGCCATTACAAAGTTTGTCACTCTGAATAACCTGATGCCAGTTAACAGTGCATGCTTTGGGGAC TCCGGAGGTGGCTTCCCCTGGGGAGCAAATGGGTTGATGTTCTCCAAACCCTGTTTCCAGCGCTCGGTGG GAGGGAGCCCACAGGTGGGAGGCCATCCCGTTGCCGCCGGCCCAGTGTTTCTAGTTCTGCACTTTGAACC GGAGAGGCTAACCTCAAAGATATTTTTTTTAACTGCATTCTATAATAAATCAGCACAATATGCTCTTCTG AAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236038 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236038 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005074 same experiment
	EMAGE:31032 same embryo
	EMAGE:30408 same embryo
	EMAGE:30410 same embryo
	EMAGE:31852 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source