EMAGE mouse gene expression spatial database

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EMAGE:30682

Zmynd8 zinc finger, MYND-type containing 8 ( MGI:1918025)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003628_01	euxassay_003628_02	euxassay_003628_03	euxassay_003628_04	euxassay_003628_05

euxassay_003628_06	euxassay_003628_07	euxassay_003628_08	euxassay_003628_09	euxassay_003628_10

euxassay_003628_11	euxassay_003628_12	euxassay_003628_13	euxassay_003628_14	euxassay_003628_15

euxassay_003628_16	euxassay_003628_17	euxassay_003628_18	euxassay_003628_19	euxassay_003628_20

euxassay_003628_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9176
Entity Detected:	Zmynd8, zinc finger, MYND-type containing 8 ( MGI:1918025)
Sequence:	sense strand is shown >T9176 AGTATTGACATTACACAGTTAACAATGCATCCACAGAGCTTGGCTGAAGAGGAAATAAAAACCGAGCAGG AGGTGGTGGAGGGAATGGATATCTCTACTCGCTCCAAAGAACCTAAGGAAGATCCTGTCTCTACAGAGAA AACGGCCCCGAAACGGAGGTTCCCCAGCCCTCCACATTCCTCCAATGGCCATTCGCCCCAAGACTCATCC ACGAGCCCCATTAAAAAGAAAAAGAAACCCGGCTTACTCAACAGTAGCAATAAGGAACAGTCAGAGCTAA GACATGGTCCGTTTTACTATATGAAGCAGCCACTCACCACAGACCCTGTTGATGTTGTACCGCAGGACGG ACGGAATGACTTCTATTGCTGGGTTTGTCACCGGGAAGGACAAGTCCTTTGCTGTGAGCTCTGTCCCCGG GTTTATCACGCTAAGTGTCTGAGACTGACATCGGAGCCAGAGGGGGACTGGTTTTGTCCTGAATGTGAGA AGATTACAGTAGCAGAATGCATCGAGACGCAGAGCAAAGCCATGACCATGCTGACCATTGAACAACTGTC CTACCTGCTCAAGTTTGCCATTCAGAAAATGAAGCAGCCAGGGACGGATGCATTCCAGAAGCCTGTTCCA TTGGAGCAACACCCTGACTATGCAGAATATATTTTCCACCCCATGGACCTTTGTACATTGGAAAAGAATG CAAAAAAGAAGATGTACGGCTGCACAGAAGCCTTCCTGGCCGATGCCAAGTGGATCCTGCACAACTGCAT TATTTTTAATGGGGGAAATCACAAGTTGACGCAAATAGCAAAAGTCGTCATCAAAATCTGTGAGCACGAG ATGAATGAAATCGAAGTCTGTCCAGAATGTTATCTTGCAGCTTGCCAAAAACGAGACAACTGGTTCTGTG AGCCCTGTAGCAATCCGCACCCTTTGGTCTGGGCAAAACTGAAAGGATTTCCATTCTGGCCAGCGAAAGC TCTGAGGGACAAAGACGGGCAGGTTGACGCCCGTTTCTTTGGACAACATGACAGAGCCTGGGTTCCAGTC AATAATTGCTACCTCATGTCTAAAGAAATCCCCTTTTCTGTGAAAAAGACTAAAAGTATCTTCAACAGCG CCATGCAAGAGATGGAAGTTTACGTGGAGAACATACGGAGGAAGTTTGGGGTTTTTAATTACTCCCCGTT CAGGACGCCCTACACGCCCAACAACCAGTACCAAATGCTGCTGGATCCCAGCAACCCCAGCGCGGGCACA GCCAAGACAGACAAACAGGAGAAGGTGAAGCTTAATTTTGACATGACAGCGTCCCCCAAGATCCTTCTGA GCAAGCCCCTTCTGAGCGGGGGTGCCGGCCGCAGGATCTCCCTGTCCGACATGCCTCGCTCCCCCACCAG TACGAACTCTTCCGTGCACACGGGCTCCGATGTGGAGCAGGACCCCGAGAAGAAGGCCCCGTCCAGCCAC TTCAGCGCAAGCGAGGAGTCCATGGACTTCCTTGATAAGAGCACAGCTTCTCCAGCCTCCACCAAGACGG GGCAAGCCGGGAGCTTGTCTGGCAGCCCAAAGCCTTTCTCTCCGCAAGCGCCGACACCCATCATGACAAA ACCCGACAAGACTTCCACCTCCACCACCGGGAGCATCCTGAACCTGAACTTGGATCGAAGCAAGGCCGAG ATGGACCTGAAGGAGCTGAGCGAGTCGGTCCAGCAGCAGTCAGCCCCCGTCCCTCTCATCTCTCCCAAGC GGCAGATTCGAAGCCGGTTCCAGCTCAACCTGGACAAGACCATAGAGAGTTGCAAAGCACAGCTAGGCAT AAATGAGATCTCAGAGGATGTTTATACAGCCGTGGAGCACAGCGATTCCGAGGACTCCGAAAAGTCGGAG AGCAGCGACAGCGAGTACGTCAGCGATGAGGAACAGAAGCCCAAGAATGAGCCCGAGGACCCCGAGGACA AAGAGGGGAGTCGGGTGGACAAAGAGGCCCCTGCCATCAAAAGGAAGCCCAAACCCACAAACCAGGTAGA GGTCAAAGAGGAAGCGAAGAGCAACTCTCCTGTCAGCGAGAAGCCGGACCCCACACCCGCCAAGGACAAG GCCAGCCCAGAGCCTGAGAAGGACTTTGTAGAGAAAGCAAAGCCATCACCTCATCCCACAAAGGACAAAC TGAAAGGAAAGGATGAAACGGATTCTCCCACAGTGCACTTGGGCTTGGATTCGGACTCGGAGAGCGAACT TGTCATAGACTTAGGAGAGGATCCTTCTGGGAGGGAGGGTCGAAAAAACAAGAAAGATCCCAAGGTGCCG TCGCCTAAGCAAGACGCTATAGGTAAACCGCCACCGTCGTCCACTTCGGCGGGCAACCAGTCTCCCCCAG AGACACCGGTACTCACCCGCTCAGCCACCCAAGCACCCGCGGCTGGGGTCACCGTGGCCGCCGCCACCAC CAGCACGATGTCTACCGTCACAGTCACGGCACCGGCCACCGCCGTCACGGGAAGCCCGGTGAAGAAGCAG AGGCCGCTCTTACCGAAGGAGACTGTCCCAGCTGTGCAGCGGGTCGTGTGGAACGCATCAAGTAAGTTTC AAACGTCCTCCCAAAAGTGGCACATGCAGAAGATACAGCGCCAGCAGCAGCAGCAGCAACAACAACAGCA GAGCCAACAGCAGAGCCAGCAGCAGCAGCCTCAGTCTTCCCAGGGGACGAGATATCAGACCAGACAGGCT GTGAAAGCTGTCCAGCAGAAGGAGGTCACCCAGAGCCCATCCACGTCCACCATCACGCTGGTGACCAGCA CACAGCCGGCAGCCCTGGTCAGCAGTTCGGGCTCAGCAAGCACCCTGGCGTCTGCAATCAATGCCGACCT TCCCATTGCCACCGCCTCGGCCGACGTGGCCGCAGACATTGCCAAGTACACCAGCAAAATGATGGATGCC ATAAAGGGGACGATGACAGAAATCTACAATGACCTCTCCAAGAACACCACTGGGAGCACAATAGCTGAGA TTCGAAGGCTGAGGATTGAGATTGAGAAACTGCAGTGGCTGCACCAGCAGGAGCTCGCTGAGATGAAGCA CAACCTGGAGTTGACCATGGCCGAGATGCGGCAGAGCCTGGAACAGGAGCGGGATCGGCTCATTGCCGAG GTGAAAAAGCAACTGGAGCTGGAGAAGCAGCAGGCGGTGGACGAGACCAAGAAGAAGCAGTGGTGTGCCA ACTGCAAGAAGGAGGCCATTTTCTACTGCTGCTGGAACACCAGCTACTGTGACTACCCCTGTCAGCAGGC CCACTGGCCCGAGCACATGAAGTCCTGTACCCAGTCGGCGACTGCCCCTCAGCAGGAAGCAGATGCCGAG GCAAGCACAGAAACAGGAAATAAGTCATCGCAGGGCAACTCCTCCAACACACAGTCAGCACCTTCAGAAC CGGCCAGCGCCCCCAAAGAGAAAGAGGCGCCAGCGGAGAAGAGCAAGGACAGTAGTAACTCGACCCTGGA TCTTTCCGGCTCCAGAGAGACGCCCTCCTCTATGCTCTTAGGCTCCAATCAAAGCTCTGTTAGCAAGAGG TGTGACAAGCAGCCTGCCTATACCCCAACCACTACAGACCACCAGCCGCACCCCAACTACCCAGCCCAGA AGTACCATTCCCGGAGCAGCAAGGCAGGTTTGTGGAGCAGCAGCGAGGAGAAGCGAGCGTCATCCCGCTC TGAGCACAGTGGAGGGACCAGCACGAAGAACCTCATGCCCAAAGAGTCCCGGGAGTCTCGGCTAGATGCC TTCTGGGACTAGGGGTGCATTGTGAGCCAGAACACCTCCCTATAGGCGGGAGAAACAGCCCAAACCACAG GAAGCAACAGAAACTGGAGAACCGCCACCTTTAGAATTTCCAACACTCAGGCTCTGCCCGTCGGCTTGGC TGCAGGACAGAGACTTGACTCCATCCCAGCTCTGGTCTTGGCTGGGATGGCACTACCCTGTGAGCCAAAC ACTTTAGGTGTAAGTAGGTACACTTGTGACGTCACTGACCTAGCGTACCTTTCCTTTTCATATCTACACT GACCTTAACTTATAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827541 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827541 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003628 same experiment
	EMAGE:29868 same embryo
	EMAGE:30646 same embryo
	EMAGE:30684 same embryo
	EMAGE:30689 same embryo
	EMAGE:30654 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source