EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30711

Man2a1 ( MGI:104669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006319_01	euxassay_006319_02	euxassay_006319_03	euxassay_006319_04	euxassay_006319_05

euxassay_006319_06	euxassay_006319_07	euxassay_006319_08	euxassay_006319_09	euxassay_006319_10

euxassay_006319_11	euxassay_006319_12	euxassay_006319_13	euxassay_006319_14	euxassay_006319_15

euxassay_006319_16	euxassay_006319_17	euxassay_006319_18	euxassay_006319_19	euxassay_006319_20

euxassay_006319_21	euxassay_006319_22	euxassay_006319_23	euxassay_006319_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35007
Entity Detected:	Man2a1, ( MGI:104669)
Sequence:	sense strand is shown >T35007 TGAATACAGGTGTTCCAAGGTGGGGTTTTCTATTTTTAAATTACCATATCAAAAATAAATGTGCCTTATT TTTTTATAAGTCTTGTTAAATCTTATTACTGTCCATATTACTGTTTGGGAGGTGGGGTATTGGGAGGTTG GGATAGGGGGTGGGTACTTTCTATGACACATAAATTATGTAAAATTTTTTGCTTGATAATGCTGGCCACA TCCTGATCTGTGTCATTACATTTGTGGTGATGTTACTCTAAACATTTTGACTATTTGAATGTACTGAGAT GTCAGAAAACGAAACAAGGGAGAAAAATATTGCTAATTAAAATATGCTGCTGCCAAGGAAACTGCAAGTT GAAGCAAGGATTTCGTAACCCACAAGATCCAAGTACTTCTTGCATTTCACAGTACTTAACCGCGTTAAAG AGAGAATGCTTTAAAAATAGACTGTTTTCTAAAATCCTCTGTGTGTAGCTCATCTTATGTTCCTTACTAA AGAACACGTTCCAAACATTTGTGTGTGGTAAACTCAGCGTATTAGTCTAGCCCATGTACCATCATGGGAC CTTTGAGGTCATTTGAGATCATTGTTTCTGTAATAGAATAATAGTATTTAATTTTGGTATAGAAAGAATG CTTTGGCTATATCCACTTTTGAAAATTCTCCTCAGTGTCTTAACCTAATTTAGTATTCTTTGACTTTAAA CCTCCACTTACCTCTTACCATTCCAGTACATTACAGAGAAAAGCATTTCTGCCTCACTGTTTATTCTGAT CGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50065. Forward Primer - name:050065_F_cDNA_Man2a1, sequence:TGAATACAGGTGTTCCAAGGTG; Reverse Primer - name:050065_N_SP6_cDNA_Man2a1, sequence:GACGATCAGAATAAACAGTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006319 same experiment
	EMAGE:32111 same embryo
	EMAGE:31559 same embryo
	EMAGE:31586 same embryo
	EMAGE:31621 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source