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EMAGE:30713

Kl ( MGI:1101771)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30713
euxassay_006318_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35009
Entity Detected:Kl, ( MGI:1101771)
Sequence:sense strand is shown

>T35009
TCCTGTTGGGGAGTAACACTCTCTCAATTACCATCTTAACACCTGGACTTTACCTGATCCAGTTTTACAA
GGTGAAGTAGAAAAATATCCAGTAAAGGTGGCCAAGAGCCCTGAGTCCAGAGCAGCCCATTAAGAAGCAC
TATTCCTACCAAATGCTGCTAATGTCAATTTACAAATATACTTAGAAAGCACATTATGGACATTTGTATT
CTTGTGAATGTTTTTGAGGTGTGCCCTAAACCCCAGATCCTTGAGGGCTTTCTCTTACCAACTTTCCTTT
CAGAGCCTGCTTGTTGGAGATTCTTCCCCAGCCCCCTTCCCCTTTCCCTCTTGCTCTGCCCCACCTCGCT
CCACCCAGCTTGCTCCAGCCCAAAGATTCTTTATTTGTTTCTCATTACCGAAGGTTGTGAGCCACCATGT
GGTTTCTGGGATTTGAACTCATGACCTCCGGAGGAGCTGTCATGCTCTTAACCAGCCCATGTTGAAGATT
CTTTTGATAAATATTCACAAAAAATAAAGATGAGCCATGAGCTGTTGGCCTCTTCGGAAGCGGAAACTGA
GTGATTTGATTGAACATCCTTTTATCTTTGACCAGACCTTGGAATGAATGCAATGACCTTTCCCACAGGA
AGAAGGAGGAGCTCTCAGTCAAACTGTAAAGAATGCCTCTTCAGAATATGCTGTCAGTGCTTGGATGCCA
TGATGTTCAACTTTCTTAGTCGATCCGGCAGCAATCACAGTGTGAGCACACTGGGAACCTGTCCTTGCGG
CCGCCGAGATCTACCGTGTGCTTCTGTGAAGAGGCTTTGACGTAGCCCCTCTTTGAGCTCTTACACCATG
CTACTGACTTCTAGAAAGGCTAATTAGGTCTTCTTCTACACCTAATACCCTAAGTCTTACTGACTCTCAC
GGGAGAAGTCTCTGTGCTACACCTGAGTGGTCTTATTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50075. Forward Primer - name:050075_F_cDNA_Kl, sequence:TCCTGTTGGGGAGTAACACTCT; Reverse Primer - name:050075_N_SP6_cDNA_Kl, sequence:CAATAAGACCACTCAGGTGTAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006318 same experiment
 EMAGE:31549 same embryo
 EMAGE:30449 same embryo
 EMAGE:30465 same embryo
 EMAGE:32119 same embryo
 EMAGE:31551 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS