EMAGE mouse gene expression spatial database

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EMAGE:30857

Sox11 SRY-box containing gene 11 ( MGI:98359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005167_01	euxassay_005167_02	euxassay_005167_03	euxassay_005167_04	euxassay_005167_05

euxassay_005167_06	euxassay_005167_07	euxassay_005167_08	euxassay_005167_09	euxassay_005167_10

euxassay_005167_11	euxassay_005167_12	euxassay_005167_13	euxassay_005167_14	euxassay_005167_15

euxassay_005167_16	euxassay_005167_17	euxassay_005167_18	euxassay_005167_19	euxassay_005167_20

euxassay_005167_21	euxassay_005167_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8597
Entity Detected:	Sox11, SRY-box containing gene 11 ( MGI:98359)
Sequence:	sense strand is shown >T8597 GCAGCCGCGGACGCGCGCGGCCAAGGACTTTGCAACTTGCCGGAGAAGGTGGAGGGGCGGGAGGGGGAGG GGCGCCCGCGCAGGAGACCGAGCGCCCAGCCCCGCCGCGGACCATGGTGCAGCAGGCCGAGAGCTCGGAA GCCGAGAGCAACCTGCCCCGGGACGCGCTGGACACCGAGGAGGGCGAGTTCATGGCGTGCAGCCCGGTGG CCCTGGACGAGAGCGACCCGGACTGGTGCAAGACGGCGTCGGGCCACATCAAACGGCCCATGAACGCCTT CATGGTGTGGTCCAAGATCGAGCGCAGGAAGATCATGGAGCAGTCGCCCGACATGCACAACGCCGAGATC TCCAAGAGGCTGGGCAAGCGCTGGAAGATGCTGAAGGACAGCGAGAAGATCCCGTTCATCAGGGAGGCGG AGCGCCTGCGCCTCAAGCACATGGCTGATTATCCCGACTACAAGTACCGGCCGCGCAAAAAGCCCAAGAC GGACCCAGCGGCCAAGCCCAGCGCGGGCCAGAGCCCCGACAAGAGCGCGGCGGGCGCCAAGGCAGCCAAG GGCCCCGGCAAGAAGTGCGCCAAGCTCAAGGCGCCTGCGGGCAAGGCGGGCGCGGGCAAGGCGGCGCAGC CGGGGGACTGCGCCGCGGGCAAGGCAGCCAAGTGCGTCTTCCTGGACGACGACGATGAAGACGACGACGA AGATGACGAGCTGCAGCTACGGCCCAAGCCGGACGCTGACGACGACGACGACGAGCCCGCGCACTCGCAC CTGCTGCCGCCGCCGACGCAGCAGCAACCCCCTCAGCTGCTGAGGCGCTACAGCGTGGCCAAGGTCCCCG CCAGCCCCACGCTCAGCAGTGCCGCCGAGTCCCCCGAGGGCGCGAGCCTGTACGACGAAGTGCGCGCGGG CGGCCGGCTCTACTACAGCTTCAAGAACATCACCAAGCAGCAGCCTCCGCCCGCGCCTCCCGCGCTGTCG CCCGCGTCCTCCCGCTGCGTGTCCACCTCCTCATCCAGCGGCAGCAGCAGCGGCAGCGGCGCCGAGGATG CAGACGACCTCATGTTCGACCTGAGCTTGAATTTCTCCCAGGGCGCGCACAGCGCCTGCGAGCAGCCACT GGGCGCGGGAGCGGCGGGGAACCTGTCCCTGTCGCTGGTGGATAAGGACCTGGATTCCTTCAGCGAGGGC AGCCTGGGTTCCCACTTCGAGTTCCCCGACTACTGCACGCCGGAGCTGAGCGAGATGATCGCGGGGGACT GGCTGGAGGCGAACTTCTCCGACCTGGTGTTCACGTATTGAGAGGCGCAGGGCTCCTCGGGAGGCAGTCG TGGCGGTCAGGATAAAGAGGATGGTGGGGGGAGGGGGAGAAGATGCTCATGGTCAGAGAGATGTGGCGCT GAGAAATTGGAAACGGTGATGAAATGTTGTTGGAGTTAAAGTGAAATGAGTAGTTTTAAACAGATTTTCC AAGTTTTTTTTTTAATTTTTATTTTTAAAAAATTTTTTGGTCTCCCCACTCTTCCCGTCCTTTATCGTGT CTCAAGGTAGTTGCACACCTAGTCTGGAGTTGTGATTTTTTCCCCACCCGTAAAAAAACGTGTTTTGTAA TTTCTATTTCTTTTTCCTGAAATTCGTGATTGCAACAAAGGCAGAGGGGACCGGGCGGGGGAGGGGAGGA GGCCCCGCTCCGGAAGGCGCTGTTTGAAGCTTGTGGCTCTTTGAAGTCTGAAAGACATCGGCAGAGGACC CTTTAGACGGCACAACTGTTACTCTAGGGAGTTGATTTTTTTTTCTTAAGAGATTTAAAAAGGAACTGGT GATTTAAAAAAAGAAAAACACAAAAAGGGACCATTGCAACCTTTTTATTTTATTTTCTTTTTTGGAGGGA GAAAGCTGATGTCTTCTATGCATCCGATTTCTTAAACTGCAGGGAGCTTGAAAAAATGCAGTCTGTACAA ACGCTTACAAAAATCATAACTGTGAACTGACTTAAGACCAGAGTTTACTTTTCAGATCAAATTGTTTATG GTTTTACAAATGTGATTTCTACTTGGCAACCTTTTTTTGGTAACTCGTTCCCTTATACCTCCTTGATTGA ATACCTTACAGCCTAGACCTCAGTACAAAAAGGTATTGAAACATTTTTGATACATAACAGACCTCAATCT TTTTTAAAAATTAATATATTTTCAGGCGTATTTTTGTACAGTGAAAAGGGAACAATCTTGCTGTGTTTTT TCAGTAAGACTTTCAGGCACTTCTTCCCTTTTGATTTCTTTTTCTTTTTTTCTCTTCTTGTTTTTGTTTT TCTTTTTTGTTTGTTTTTTTAGCATGCAAATATGTTGGTACGTTATGTCCTGGTTTAAAAGGATTAAAAA TTTAAAAGTAATCCTTGCATCTAAAGGCCTTGTGGTTTAAAAAAAAAAGCAAACTTTTTTGTACAGCTAT AGTAGAGATTTGTTCAATATTTGTAGGTAAAGATTTATTGAAAATGGTGACCTAGACCTCAGAGCTGTTC TTAGTTTAAGACTGTATGTACTGTATCATAGTAGGACCTATGTATCTCATCGCTGTGATGTGTGGACGGG GCCCCAGACAGAAGGTTTGAAACTGGATACGCGATTTTTATCAAAAAGAAAAAGGAAAGGCACATAGTTT AAAATTTTCTCATTTTGTGCAATATAATTAAATAAACAGACCATTGCATATTTAGTAGCAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716171 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716171 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005167 same experiment
	EMAGE:30946 same embryo
	EMAGE:30866 same embryo
	EMAGE:30893 same embryo
	EMAGE:29224 same embryo
	EMAGE:31337 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source