EMAGE mouse gene expression spatial database

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EMAGE:30893

Ccnd2 cyclin D2 ( MGI:88314)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005143_01	euxassay_005143_02	euxassay_005143_03	euxassay_005143_04	euxassay_005143_05

euxassay_005143_06	euxassay_005143_07	euxassay_005143_08	euxassay_005143_09	euxassay_005143_10

euxassay_005143_11	euxassay_005143_12	euxassay_005143_13	euxassay_005143_14	euxassay_005143_15

euxassay_005143_16	euxassay_005143_17	euxassay_005143_18	euxassay_005143_19	euxassay_005143_20

euxassay_005143_21	euxassay_005143_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 17 moderate expression: see section 14 15 16

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 05 06 07 08 09 10 11 13 14 15 16 17 18 weak expression: see section 03 04 19

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 weak expression: see section 04

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 weak expression: see section 09

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 15

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

rest of cerebellum marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 weak expression: see section 09

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8598
Entity Detected:	Ccnd2, cyclin D2 ( MGI:88314)
Sequence:	sense strand is shown >T8598 CGAGCTGAGGAGAGCCGGGCAGTTCGGAGGGAAGGACCGGTGCGAGTCAGGCGGCCCTTGAGGCTCCGCT CGCCCACCTTCCACTCTTCTCTCTCTCTCTCCCTCTCTCTCTTTGCCATTTCTTTCCTCTCCCAAATCTC CCATTCAGCCAAAGGAAGGAGGTAAGGGAAGCACTCCCCGACCCCCCCGCACCTCCAAAAAATAATAATA ATAAAAAAAATTTACAGTCGGGACCGAGTGGTGGCCGGCTGGCTATGGAGCTGCTGTGCTGCGAGGTGGA CCCGGTCCGCAGGGCCGTGCCGGACCGCAACCTGCTGGAAGACCGCGTTCTGCAGAACCTGTTGACCATC GAGGAGCGCTACCTCCCGCAGTGTTCCTATTTCAAGTGCGTGCAGAAGGACATCCAACCGTACATGCGCA GGATGGTGGCCACCTGGATGCTAGAGGTCTGTGAGGAACAAAAGTGTGAAGAAGAGGTCTTTCCTCTGGC CATGAATTACCTGGACCGTTTCTTGGCTGGAGTCCCGACTCCTAAGACCCATCTTCAGCTCCTGGGTGCA GTGTGCATGTTCCTAGCTTCCAAGCTGAAAGAGACCATCCCGCTGACTGCGGAAAAGCTGTGCATTTACA CCGACAACTCTGTGAAGCCCCAGGAGCTGCTGGAGTGGGAACTGGTAGTGTTGGGTAAGCTGAAGTGGAA CCTGGCCGCAGTCACCCCTCACGACTTCATTGAGCACATCCTTCGCAAGCTGCCCCAGCAAAAGGAGAAG CTGTCCCTGATCCGCAAGCATGCGCAGACCTTCATCGCTCTGTGCGCTACCGACTTCAAGTTTGCCATGT ACCCGCCGTCGATGATTGCAACTGGAAGCGTGGGAGCAGCCATCTGTGGGCTTCAGCAGGATGATGAAGT GAACACACTCACGTGTGATGCCCTGACAGAGCTGCTGGCCAAGATCACCCACACTGATGTGGATTGTCTC AAAGCCTGCCAGGAGCAAATCGAAGCTCTGCTGCTGAACAGCCTGCAGCAGTTCCGTCAAGAGCAGCATA ACGCCGGATCCAAGTCTGTGGAAGATCCGGACCAAGCCACCACCCCTACAGACGTGCGGGATGTTGACCT GTGAGGAAGCCATTCGGGCGGCAAGAGAGAGGCGTGTTCGTCATCTGCTAGCCCCTTCTCTCTCTAGTTA TGTCTTGTTCTTTGTGTTTTTAGGATGAAACTTCAAAAAAGAAAAAAAAAAAACAAAAAACAAAAACAAA ATCTGCCCCCACCTAGATCCTATTTAAAGGTCTTTTAGAAGTGAGAGGAAAAGGCCGTATAGTTGTAACG CATGTGGTGCCTGTTCAAAGTCCTGCATAAGGGGATCCCTGTACACTCGAACCGTTATTATTTGATGATG TAAAAGTCATAGTGAGATGCTTACAGGAGAACCCGCAGACTAGCTAGAGAGTGTGCGTACCTGCAACATG GGAACGAATTAGAGGAGACTGTCTTTTGTGCTTGTGACCTAGTGCATATGCCCCCTTTAGTAGAATTGCA AGGAACCGTGGGTGCCAGGACCGCATGAGTAGTTTGCAAAGCAGTGAACCCAAGGAGGAATCAGAAACGA GAAGGGACATGCCGGGGAGGTTGCGCAAAACCACCCAACCACATGACTGAACCATTTTGGATGTAAGAAG CGCCTTGATGCCTCACCACGTGTTCCCAAGACTGAGCGTCCGGGTCTCTCCGTCGCATACTCTTGCTGAC TAAAGTAGCTGCTACCTAAGGGATATGTCTTTTGCCAGTTGGACACAGGTGAACGGCTCCTAAGTCTCAT GTTTGGTGAATTTGGGCTTCTACTTCCAAACATGGTTCATTGTAGATGTATTTTACTGTTATCTGATGGA GGGAATTCGCTATGGGCCATGACCAAAACGCATCTAAATGGGGGCAGATGGAGACCATAGACGGAATCTA AAACAAAGCTGCCTTGTGTAAGGGAAGAGGGTGAGGTGATCTTTAATCCCCTTGTTAAGAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5716186 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5716186 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005143 same experiment
	EMAGE:30946 same embryo
	EMAGE:30857 same embryo
	EMAGE:30866 same embryo
	EMAGE:29224 same embryo
	EMAGE:31337 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source