EMAGE mouse gene expression spatial database

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EMAGE:30933

Taf9 TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1888697)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002090_06	euxassay_002090_07	euxassay_002090_08	euxassay_002090_09	euxassay_002090_10

euxassay_002090_11	euxassay_002090_12	euxassay_002090_13	euxassay_002090_14	euxassay_002090_15

euxassay_002090_16	euxassay_002090_17	euxassay_002090_18	euxassay_002090_19	euxassay_002090_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 07 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4631
Entity Detected:	Taf9, TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor ( MGI:1888697)
Sequence:	sense strand is shown >T4631 CCCACGCGTCCGGCGTGAGCGGAGGCTTTGGCTGCAGGGGAAGGAGGACTATGAAGCTTCCGAATATCCT GCTCACCGGTACACCAGGGGTTGGAAAAACCACACTAGGCAAAGAACTTGCATCAAGATCAGGCCTGAAG TACGTTAACGTGGGAGATCTAGCTCGAGAAGTCTGATCAGCGGATATCATGGAGTCTGGCAAGATGGCGT CTCCCAAGAGCATGCCGAAAGATGCACAGATGATGGCACAAATCCTGAAGGATATGGGGATTACAGAATA TGAGCCAAGAGTTATAAATCAGATGCTGGAGTTTGCCTTCCGATATGTGACAACAATTCTAGATGATGCT AAAATTTATTCCAGCCATGCTAAGAAAGCTACTGTTGATGCAGATGACGTACGACTGGCAATCCAGTGCC GTGCTGACCAGTCTTTCACTTCTCCACCCCCGAGAGATTTCTTATTAGATATTGCACGACAAAGAAATCA AACCCCTTTGCCACTGATCAAGCCATATTCAGGTCCTAGATTGCCACCTGATAGGTATTGCTTAACTGCC CCAAACTATAGGCTTAAGTCTTTACAGAAAAAGGCACCTGCTCCTGCAGGAAGAATAACAGTTCCAAGGT TAAGTGTTGGTTCAGTTTCTAGTAGACCAAGTACTCCCACCCTAGGTACACCGACTCCACAAACCATGTC TGTTTCAACTAAAGTAGGCACCCCAATGTCCCTCACAGGACAGAGGTTTACAGTACAGATGCCTGCTTCT CAGTCCCCTGCTGTAAAAGCTTCTATACCTGCAACATCAACAGTTCAGAATGTTCTTATTAATCCATCGT TAATTGGGTCCAAAAACATTCTTATTACTACTAATATGGTATCACAGAATACAGCCGAGTCAGCAAATGC ACTGAAACGGAAACGTGAAGACGATGATGATGACGATGATGACGATGATGATGATGACTATGATAATATG TAGTCTAGCCACGATGCATGTAACCTGTGTTCTTGGACTTGGATCCATTGTACTACAAGTTAAAAAGCAT GTTAGATGTTTTAAAGCTGTACTTTAGAAAGTGGTGTTTCATACGTATGCTTTGCTTTTTGATTAAAATG TTCAGTTTTACTAGAACTAGTGATGGTTTTTCATCAACCTTTATGCACAAAAAATAAAATTGTCATTTAT CAGTTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5006430 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5006430 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002090 same experiment
	EMAGE:29483 same embryo
	EMAGE:29497 same embryo
	EMAGE:30909 same embryo
	EMAGE:29257 same embryo
	EMAGE:31325 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source