EMAGE mouse gene expression spatial database

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EMAGE:30943

Serpinc1 ( MGI:88095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004038_01	euxassay_004038_02	euxassay_004038_03	euxassay_004038_04	euxassay_004038_05

euxassay_004038_06	euxassay_004038_07	euxassay_004038_08	euxassay_004038_09	euxassay_004038_10

euxassay_004038_11	euxassay_004038_12	euxassay_004038_13	euxassay_004038_14	euxassay_004038_15

euxassay_004038_16	euxassay_004038_17	euxassay_004038_18	euxassay_004038_19	euxassay_004038_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4937
Entity Detected:	Serpinc1, ( MGI:88095)
Sequence:	sense strand is shown >T4937 ATTTTAGTGAAGTGGTGAGATATAAGACATAATCAGAAGACATATCTACCTGAAGACTTTAAGGGGAGAG CTCCCTCCCCCACCTGGCCTCTGGACCTCTCAGATTTAGGGGAAAGAACCAGTTTTCGGAGTGATCGTCT CAGTCAGCACCATCTCTGTAGGAGCATCGGCCATGTATTCCCCTGGGGCAGGAAGTGGGGCTGCTGGTGA GAGGAAGCTTTGTCTCCTCTCTCTGCTCCTCATCGGTGCCTTGGGCTGTGCTATCTGTCACGGAAACCCT GTGGACGACATCTGCATAGCGAAGCCCCGAGACATCCCCGTGAATCCCTTGTGCATTTACCGCTCCCCTG GGAAGAAGGCCACCGAGGAGGATGGCTCAGAGCAGAAGGTTCCAGAAGCCACCAACCGGCGGGTCTGGGA ACTGTCCAAGGCCAATTCGCGATTTGCCACTAACTTCTACCAGCACCTGGCAGACTCCAAGAATGACAAC GACAACATTTTCCTGTCACCCTTGAGCATCTCCACTGCTTTTGCTATGACCAAGCTGGGTGCCTGTAACG ACACTCTCAAGCAGCTGATGGAGGTTTTTAAATTTGATACCATCTCCGAGAAGACATCCGACCAGATCCA CTTCTTCTTTGCCAAACTGAACTGCCGACTCTATCGAAAAGCCAACAAGTCCTCTGACTTGGTATCAGCC AACCGCCTTTTTGGAGACAAATCCCTCACCTTCAACGAGAGCTATCAAGATGTTAGTGAGGTTGTCTATG GAGCCAAGCTCCAGCCCCTGGACTTCAAGGAGAATCCGGAGCAATCCAGAGTGACCATCAACAACTGGGT AGCTAATAAGACTGAAGGCCGCATCAAAGATGTCATCCCACAGGGCGCCATTAACGAGCTCACTGCCCTG GTTCTGGTTAACACCATTTACTTCAAGGGCCTGTGGAAGTCAAAGTTCAGCCCTGAGAACACAAGGAAGG AACCGTTCTATAAGGTCGATGGGCAGTCATGCCCAGTGCCTATGATGTACCAGGAAGGCAAATTCAAATA CCGGCGCGTGGCAGAGGGCACCCAGGTGCTAGAGCTGCCCTTCAAGGGGGATGACATCACCATGGTGCTC ATCCTGCCCAAGCCTGAGAAGAGCCTGGCCAAGGTGGAGCAGGAGCTCACCCCAGAGCTGCTGCAGGAGT GGCTGGATGAGCTGTCAGAGACTATGCTTGTGGTCCACATGCCCCGCTTCCGCACCGAGGATGGCTTCAG TCTGAAGGAGCAGCTGCAAGACATGGGCCTCATTGATCTCTTCAGCCCTGAAAAGTCCCAACTCCCAGGG ATCGTTGCTGGAGGCAGGGACGACCTCTATGTCTCCGACGCATTCCACAAAGCATTTCTTGAGGTAAATG AGGAAGGCAGTGAAGCAGCAGCGAGTACTTCTGTCGTGATTACTGGCCGGTCACTGAACCCCAATAGGGT GACCTTCAAGGCCAACAGGCCCTTCCTGGTTCTTATAAGGGAAGTTGCACTGAACACTATTATATTCATG GGGAGAGTGGCTAATCCTTGTGTGAACTAAAATATTCTTAATCTTTGCACCTTTTCCTACTTTGGTGTTT GTGAATAGAAGTAAAAATAAATACGACTGCCACCTCACGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125534 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125534 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004038 same experiment
	EMAGE:30031 same embryo
	EMAGE:30947 same embryo
	EMAGE:30897 same embryo
	EMAGE:30334 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source