EMAGE mouse gene expression spatial database

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EMAGE:31171

Klf6 Kruppel-like factor 6 ( MGI:1346318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003793_01	euxassay_003793_02	euxassay_003793_03	euxassay_003793_04	euxassay_003793_05

euxassay_003793_06	euxassay_003793_07	euxassay_003793_08	euxassay_003793_09	euxassay_003793_10

euxassay_003793_11	euxassay_003793_12	euxassay_003793_13	euxassay_003793_14	euxassay_003793_15

euxassay_003793_16	euxassay_003793_17	euxassay_003793_18	euxassay_003793_19	euxassay_003793_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 06 07 10 11

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 06 07 08 09 11 12 13 14

thalamus mantle layer

moderate

regional

moderate expression: see section 05 06 14 weak expression: see section 07 08 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7040
Entity Detected:	Klf6, Kruppel-like factor 6 ( MGI:1346318)
Sequence:	sense strand is shown >T7040 GTCCGCTGTTTGCAAATATTGCGTGGGCTCGGCGCGCTGCGGGCTGCGGGAGGGTCCGGACCCGGCGTCC GATTGCAGCGCCATCCAGTTTGCATGAAACTTTCACCTGCGCTCCCGGGAACAGTTTCTGCTCGGACTCC TGATCGTTCACCTCCCTGTTTTCCCGACAGCGAGGACTGTCTTTTCCAACCCGACATGGATGTGCTCCCA ATGTGTAGCATCTTCCAGGAACTACAGATTGTGCACGAAACGGGCTACTTCTCGGCTCTGCCGTCCCTGG AGGAATATTGGCAACAGACCTGCCTGGAGTTGGAACGCTATCTTCAGAGTGAGCCCTGCTACGTGTCAGC CTCTGAGATAAAATTTGACAGCCAGGAAGACCCGTGGACCAAATTCATTCTAGCTCGGGGGGAGAAGAAG GAGGAATCAGAACTGAAGATTTCTTCTAGTCCCCCAGAGGACTCTCTGATCAGCTCCAGCTTTAATTATA ACTTAGAGACCAATAGCCTGAACTCTGATGTCAGCAGTGAGTCTTCGGACAGTTCAGAGGAACTTTCACC CACGACCAAATTTACCTCTGATCCCATTGGTGAAGTCTTAGTCAATTCAGGAAATCTGAGTTCCTCCGTC ATTTCCACACCTCCATCTTCTCCAGAAGTGAACAGGGAATCTTCTCAACTATGGGGTTGTGGGCCAGGAG ACCTGCCCTCACCTGGGAAGGTTCGAAGTGGGACCTCTGGGAAGTCTGGTGACAAGGGTAATGGCGACGC CTCCCCAGATGGCAGAAGACGGGTACATCGGTGCCACTTTAATGGCTGCAGGAAAGTTTACACTAAAAGC TCCCACTTGAAAGCACATCAGCGCACTCACACAGGAGAAAAGCCTTACAGATGCTCATGGGAAGGTTGTG AGTGGCGTTTTGCAAGAAGTGATGAGTTGACCAGACACTTCCGAAAGCATACTGGTGCCAAGCCTTTTAA ATGCTCCCACTGTGACAGGTGTTTCTCCAGGTCTGACCACCTGGCCCTGCACATGAAGAGGCACCTCTGA AGGAGCAGAGGGACGAATCCTGTAGGCTAAAAGAGGCTTCCAGGCTAAGAGGCGGCCATGGAAGGAGGGA TGCCTGTAACAGCCAAAGCATGCCATTTTGCTTCCTATCCAGTTACCTCCAGGGGCCTCTCTTTGGAAGG TCTTTTGAGGGCTACAAAAGTCATGTCAGGAGTGGCATAGCACCCATGGTGCATGGTGTTTGGGTGACCC CGGACTCACCACTGGTTCCTAACCTTCTGAGAGGCTCTAAGCTTTTGGCCGTGAGCATGCGCACTGAGAA TGTTAGTGGGTGGGATGGTTGTGTTGAGGATCTATTACTGACTGTATGGTGAGGCAGACTTTTTTTTTCT CCCCCTTTGTGGTATCAAATAACTGCAAGAGACAGAAAAAAAAAAAAAGCAGTTTGAATGTTTTGTGTGT GAGGAGTATACCAAGGGATGAGTTGACCACCAATCATTTCCTGAAGGGTGTCTGCACCTTAGAAAAAAAT AAACAAAAACAACAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4008402 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4008402 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003793 same experiment
	EMAGE:31738 same embryo
	EMAGE:31087 same embryo
	EMAGE:31217 same embryo
	EMAGE:31214 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source