EMAGE mouse gene expression spatial database

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EMAGE:31203

Dppa4 ( MGI:2157525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006817_01	euxassay_006817_02	euxassay_006817_03	euxassay_006817_04	euxassay_006817_05

euxassay_006817_06	euxassay_006817_07	euxassay_006817_08	euxassay_006817_09	euxassay_006817_10

euxassay_006817_11	euxassay_006817_12	euxassay_006817_13	euxassay_006817_14	euxassay_006817_15

euxassay_006817_16	euxassay_006817_17	euxassay_006817_18	euxassay_006817_19	euxassay_006817_20

euxassay_006817_21	euxassay_006817_22	euxassay_006817_23	euxassay_006817_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 15 16 17

vomeronasal organ

weak

regional

weak expression: see section 11 14

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14 15

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 16 17 18

ovary

moderate

regional

moderate expression: see section 05 06 07 18 19 weak expression: see section 20

thyroid gland

weak

regional

weak expression: see section 10 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8140
Entity Detected:	Dppa4, ( MGI:2157525)
Sequence:	sense strand is shown >T8140 GGAGGATTCATCTATTCTCAAGATGGAGACTGCTGGAGACAAGAAGTGGAGCGCAGAAGAGCCGAAGGAA GAAGTGGAATTGCAGATGTCTAGTCAACCAGGCACGGCTCCTGCAAAGGCTAAAGCAACGGGGAAAAAAC AAAAGAAGTCGGAGACAGATAATGGTTGTAAACCAAAGGAGGGAAGACCACAAGACACTGAGACGCCAGG ACAGACTCGTAGGAAGGTACCAATTCCTCCTATTCCAGAGTATCTGCCCCCAGTGAACCTGATTCACCGA GATGTTTTGCGGGCATGGTGCCAGAAGAAACGAGTGAGCAGCAAAGGCCAGAAATTAGATGCTTATAAAC GACTCCTTGCAAGGGCTTTCCCAGAACAAATGCTGGAGTTGAGGAACGTCCCTGACTCAGCCAAAGACGC CAGGTTGAAGACAGCTCACAAAAAAATGAAGACTGAACCGGGGGAGGAGTCTGAGGTGACAGTTCCTCTG GAAATGGTCCCTGTGCCAGAGGAGCAGATACCTGCCCTCATTGACCCTCCTATGCTCTATGAGGAAGTCA GCACCACCGTAGTGACTACACCTGCCACTGAGGCCGTGTTAGCATCTTGGGCCAGAATTGCATCCAATGC TAAGAAGTACGAGGCAGTGCCAGCCGATGCTTCGTCCTCATCAGAAGTCAAAGGGGAAATGTGGTGTGTG GTTCATGGGACCAGCCTTCCTGGGAACTCGCGTGGTTGGGTTCGGCTGCAGTTCCACGCTGGACAAGCCT GGGTACCCGATAAGAAAGGAAAAGCCATTGCCCTCTTCCTGCTTCCGGCCTGCACATTTCCACCCCCACA CCTGGAGGACAACATGCTGTGCCCCAAGTGTGTTCATAAGAACAAGATCTTGACTAAGAGCCTCGAAGGA TAAAGGACAGATCTCTTGTTTGAGCTAGCTGCTGGCGTCACTCACTCTCGTGGTGTGATCAGCAGAGCTG CGGCTGACGCTGAGACTGATAGAAATCTACATCTCTGCCTTTGAAGGTGCCTTTCTCATCCAGAACTTAA GGGATAGTGTAAGTTAGTTGATGCTTAAGTTCACGCAGTTTGAAAAAAGCCAGCGAGGCCACCTCTGCTC GCTGTGATGACTGATCACTGAGAGTGAACTCTTACTCTTTTGTGAAGATAAAAATACATCCTATGTGTTT TGCTGCAAGTAGGTCCTGGGGCAGGAGGATGATATCTCCAGATAACAATAGTTGCTAGTCCTGTCACCAG CACAACGGGCGTCATAACCAGTTCAGCAGTCAACTGTAAGGACTTGGCTTATACCTGTAGCCTCCGCATG CATGTCCTCTTCCCTGGGTTGTAGTGTGTTGTTAGTGTTTAATACTTGTGTTTGTTTTCTGCTGTTGTAA AATATTACACTAAATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30024281 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30024281 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006817 same experiment
	EMAGE:31381 same embryo
	EMAGE:31440 same embryo
	EMAGE:31477 same embryo
	EMAGE:29414 same embryo
	EMAGE:29525 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source