EMAGE mouse gene expression spatial database

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EMAGE:31271

Arhgdig Rho GDP dissociation inhibitor (GDI) gamma ( MGI:108430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000468_01	euxassay_000468_02	euxassay_000468_03	euxassay_000468_04	euxassay_000468_05

euxassay_000468_06	euxassay_000468_07	euxassay_000468_08	euxassay_000468_09	euxassay_000468_10

euxassay_000468_11	euxassay_000468_12	euxassay_000468_13	euxassay_000468_14	euxassay_000468_15

euxassay_000468_16	euxassay_000468_17	euxassay_000468_18	euxassay_000468_19	euxassay_000468_20

euxassay_000468_21	euxassay_000468_22	euxassay_000468_23	euxassay_000468_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16 17 18 19 weak expression: see section 11

facial vii ganglion

strong

regional

strong expression: see section 04 moderate expression: see section 21 22

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 09 10 17 18 weak expression: see section 07

vagus x ganglion

moderate

regional

moderate expression: see section 09 19

upper jaw molar

moderate

regional

moderate expression: see section 13 14 weak expression: see section 10 11

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 moderate expression: see section 03 06 07 08 09 17 18 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 15 16 17 18

ventral grey horn

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 14 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3821
Entity Detected:	Arhgdig, Rho GDP dissociation inhibitor (GDI) gamma ( MGI:108430)
Sequence:	sense strand is shown >T3821 TGGCCTCGAGCCAGATTCGGACGAGGCAAATGTCTGCATCACACCTATCGTCGGGGACTGCGTGTGGACA AGGCCATCTTCATGGTGGGAAGCTACGGCCCCAGAGCCCAGGAGTATGAATTTGTGACTTCAGTGGAGGA AGCACCAAGAGGAGCATTGGCACGTGGCCTCTACGTGGTCAGGTCCCTCTTCACTGATGACGACAGGCTG AACCACCTGTCCTGGGAGTGGCACCTCCATGTCTGCCAGGACTGGAAGGACTGAACCCCACTTGGGGTCT GTATTCCCAATTTTCTTGTCAGTTGCAGAGACCTGCAAGCATTNCCTANACCTCCCAGTTGGTGACCA
Notes:	The probe template was PCR amplified from IMAGE:407050 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:407050 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000468 same experiment
	EMAGE:30316 same embryo
	EMAGE:30289 same embryo
	EMAGE:31223 same embryo
	EMAGE:30286 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source