EMAGE mouse gene expression spatial database

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EMAGE:31274

Spink4 ( MGI:1341848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002408_01	euxassay_002408_02	euxassay_002408_03	euxassay_002408_04	euxassay_002408_05

euxassay_002408_06	euxassay_002408_07	euxassay_002408_08	euxassay_002408_09	euxassay_002408_10

euxassay_002408_11	euxassay_002408_12	euxassay_002408_13	euxassay_002408_14	euxassay_002408_15

euxassay_002408_16	euxassay_002408_17	euxassay_002408_19	euxassay_002408_20	euxassay_002408_21

euxassay_002408_22	euxassay_002408_23	euxassay_002408_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 16 17 18 19 20

trigeminal v ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 11 19 20 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 16 weak expression: see section 10 11 17 18

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 13 14 15

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 14

pons ventricular layer

moderate

regional

moderate expression: see section 14 15

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3022
Entity Detected:	Spink4, ( MGI:1341848)
Sequence:	sense strand is shown >T3022 TGGCCTCGAGCAGATTCGTCGACTCACTTGACTGCAGTCACATAGCTCACAAGCAGCATGGCTATGCATC TGTGGCTGGTCACCCTGACCTTGGTTCCCCTCCTTGGCATGGACAGGGAACTGATGGTGTCAGCTGGGAG CCTTGTTTTCCCCAGAATGCCCTTCTGTGAGCACATGGCTGAGCTTCCAAACTGTCCCCAGACACCCAAC CTGATCTGTGGCACGGATGGGCTCACATATGAAAATGAATGCCACCTTTGCCTGACCCGGATGAAAACCA TGAAGGACATTCAAATCATGAAGGATGGCCAATGCTGAGCTCAGAGGTGCACTCAAGCCATGAAACTTCA TGCTGGAGAACAGTGATGCCCCAGAGGCTATGACATGAAATAAAAGATGCAGCCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1545489 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1545489 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002408 same experiment
	EMAGE:31154 same embryo
	EMAGE:31267 same embryo
	EMAGE:29448 same embryo
	EMAGE:30301 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source