Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31379

Smoc1 SPARC related modular calcium binding 1 ( MGI:1929878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379
euxassay_003378_01 euxassay_003378_02 euxassay_003378_03 euxassay_003378_04 euxassay_003378_05
EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379
euxassay_003378_06 euxassay_003378_07 euxassay_003378_08 euxassay_003378_09 euxassay_003378_10
EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379
euxassay_003378_11 euxassay_003378_12 euxassay_003378_13 euxassay_003378_14 euxassay_003378_15
EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379 EMAGE:31379
euxassay_003378_16 euxassay_003378_17 euxassay_003378_18 euxassay_003378_19 euxassay_003378_20
EMAGE:31379 EMAGE:31379
euxassay_003378_21 euxassay_003378_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
saccule
moderate moderate
regionalmoderate expression: see section 05 06 07 08 17 18 19 20
hip mesenchyme
strong strong
regionalstrong expression: see section 01 02 03
testis medullary region
moderate moderate
regionalmoderate expression: see section 06 07 08 09 19 weak expression: see section 20 21
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 21 22
heart ventricle
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16
knee mesenchyme
strong strong
regionalstrong expression: see section 01 02 03
midbrain meninges
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 08 21 22
axial skeleton
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19
axial skeleton tail region
strong strong
regionalstrong expression: see section 14 15 16
telencephalon meninges
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 22
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 10
nose
weak weak
regionalweak expression: see section 06 07 08 09 16 17 18
lower leg mesenchyme
strong strong
regionalstrong expression: see section 01
axial musculature
strong strong
regionalstrong expression: see section 07 22 moderate expression: see section 05 06 08 20 21
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 05 06 07 08 09 11 12 13 15 16 17 18 19 20 moderate expression: see section 14
cochlea
moderate moderate
regionalmoderate expression: see section 05 06 07 08 17 18 19 20
pancreas
strong strong
regionalstrong expression: see section 07 08 10 11 12 13 16 moderate expression: see section 14 15
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 08 21 22
upper leg mesenchyme
strong strong
regionalstrong expression: see section 04 05 06 19 22
telencephalon ventricular layer
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
midbrain ventricular layer
strong strong
homogeneousstrong expression: see section 08 09 10 11 12 15 16 moderate expression: see section 13 14 17
spinal cord meninges
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14
naris
moderate moderate
regionalmoderate expression: see section 09 10 15 16
nasal septum
moderate moderate
regionalmoderate expression: see section 12 13
ventral grey horn
moderate moderate
regionalmoderate expression: see section 13 14 15 16 weak expression: see section 12
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 05 06 21 22
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 11 12 13 15 16 moderate expression: see section 10 14
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
diencephalon meninges
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
pons ventricular layer
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 11 12 13 15 16 17 18 19 20 moderate expression: see section 10 14
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 21 22
pons mantle layer
strong strong
regionalstrong expression: see section 06 18
hyoid
moderate moderate
homogeneousmoderate expression: see section 13 14 15
hindbrain meninges
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 20
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 15 16
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 20 21 22
aorta
strong strong
regionalstrong expression: see section 12 moderate expression: see section 13 14 15
adrenal medulla
strong strong
homogeneousstrong expression: see section 08 09 10 17 18 moderate expression: see section 11 19
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6759
Entity Detected:Smoc1, SPARC related modular calcium binding 1 ( MGI:1929878)
Sequence:sense strand is shown

>T6759
GCCGCGGCCACGCCGAGCGCAGCAGGCGCCACGCGCAGAGCACACGCTCGCACTCCAGCTCCGGGCCTCC
GCCGTTCATGACCGTGACCCCGGGCGCAGGCTCCTCCAGCCCCTGCCGCTGGCTCTTCGGCCACTCCCAG
CTGCTGCAAGGGTCCCCACCTCCCCAAGTGCTTTGAAGCTAGCCTGAGCCCCCCTGCCCGCTGCCCGCTC
GTGGCTGCCCGGCTCCGCTGGCACCATGCTGCCCGCGCGCGTCCGTCTGCTCACGCCCCACTTGCTGCTC
GTGTTGGTGCAGCTGTCCCCGGCCGGCGGCCACCGCACCACCGGCCCCAGGTTCCTAATAAGTGACCGTG
ACCCTCCGTGCAACCCACACTGTCCCAGGACTCAGCCCAAGCCCATCTGCGCCTCTGACGGCAGGTCCTA
CGAGTCCATGTGTGAGTACCAGAGAGCCAAGTGCCGAGACCCAGCCCTGGCCGTGGTCCATCGAGGTCGA
TGCAAAGATGCTGGCCAGAGCAAGTGTCGCCTGGAGAGGGCTCAGGCCTTGGAACAGGCCAAGAAGCCTC
AGGAGGCTGTGTTTGTCCCAGAGTGTGGCGAGGATGGTTCCTTCACACAGGTGCAGTGCCATACGTACAC
AGGGTACTGCTGGTGTGTCACCCCAGATGGCAAGCCCATCAGTGGTTCTTCCGTGCAGAATAAAACTCCT
GTATGTTCAGGTCCAGTTACCGACAAGCCCTTGAGCCAGGGTAATTCAGGAAGAAAAGATGATGGCTCTA
AACCCACGCCCACGATGGAGACCCAACCTGTGTTCGATGGAGATGAAATCACAGCCCCCACCTTATGGAT
TAAGCACTTGGTAATCAAAGACTCCAAGTTGAATAACACCAACGTAAGAAATTCAGAGAAAGTCCATTCC
TGTGACCAGGAAAGACAGAGTGCCCTGGAAGAGGCCCGGCAGAATCCCCGAGAGGGCATTGTGATCCCCG
AGTGTGCTCCTGGTGGGCTCTATAAGCCGGTGCAATGCCACCAATCCACAGGCTACTGTTGGTGCGTCCT
AGTAGACACAGGGCGCCCATTGCCGGGGACTTCCACACGCTATGTGATGCCAAGTTGCGAGAGTGACGCC
AGAGCCAAGAGTGTAGAGGCCGATGACCCTTTCAAGGACAGGGAGTTGCCAGGTTGTCCTGAAGGGAAGA
AGATGGAATTTATTACCAGCCTGCTGGATGCCCTCACCACAGACATGGTTCAGGCCATTAACTCAGCAGC
GCCCACTGGAGGTGGGAGGTTCTCAGAGCCAGACCCCAGCCACACCCTGGAGGAGCGAGTGGCACACTGG
TACTTCAGCCAGCTGGATAGCAACAGCAGCGATGACATTAACAAGCGGGAGATGAAGCCGTTCAAGCGCT
ATGTAAAGAAGAAAGCCAAGCCCAAGAAATGTGCCCGGCGCTTCACCGACTACTGCGACCTGAACAAGGA
TAAGGTCATCTCACTGCCTGAGCTGAAGGGCTGCCTGGGTGTTAGCAAAGAAGGTGGTAGCCTTGGCAGC
TTCCCTCAGGGAAAACGAGCAGGCACAAACCCGTTCATCGGACGCCTTGTCTAAAGAGTGGAAAAAGCAA
AAGGCCCATGGAAACTCCGAGGAGACAGGACTGACCGTCAGACACCTAACCCTCAGCGCTGCCCGTGGCC
CAGCCACAGTCTGTGTAACATAAGTGGTGCCCGCCATGTTTGCACTTTTAATAACTCTTACGTGTGTGGT
TTGTTTCTGGTTCCATTTGTAAACACCAATTATCTAATACCATAGCAGGAACAGGAAAGGGAAGAAAAGC
TGTTTATTCTCTCTTTTATTGTTAAGGTTTTTTGGATCTGCTACTGACATCTTTTAGGTTGGGGGGGGGG
GGCGAGGGACGGGGTCGAGAAGAAAGAGATTTATATACTGTATATAAATATATATGTAAATTGTATAGAT
CTTTTGTACAGGCATCGACATCGCTGTTTGTCCCTCCCTTCTACTTCCTCTGGACTCCCAGTCCAGGTCT
CTCCTTAGCTTCCCCAAGTTTCACTGTATATGGACTCTGAGAGAATAGATGGGAGCCCTGTGCTTGTGCT
GTGGATAGCATGTTTTCTTCTGGTGGGAAGAGGGTACTGGTCCATTAGCTGTCAAAGTCCAGTCACTTGG
CCATTGGCTGGGTAGGAGGGGAGCCCAGTTCCTGTGCTGGCCAGCATGGATTTCAGATCTCCGGTGGCCC
ACATGTCCATGCTATCCAGATCCCTTGGCAGTCTACAGTGAATAATGGAGCAATCTCTACCTGAAGGGGC
TGGATTTGGCTGCTTTAAACATCTCTACCCATGTTTAGCATGTCCCCCGTGTAGGACATGAGAGGTGAGA
CCCCAAGCATGTGAGGAAAAGGACACCCAGCACCAGCAGGGTTCTCCTGCAGCAGCAGGTGTTGGGCATC
TGAGGAGATGGTGAAGAGGACTGAAGACCTGAACCAGCCCTTCTGCAAACCCACAAGAGAGCCTCCACAA
GCATGTAGCTCCCAACACTAGAGTCCAACTAGGTTTTACTTTCGTGCTCTCCCTCCATCTCTTGGATCCT
CACCGATTGGGTCAGCCACCTTGTTGGCCAATCCCGGAGTTGCTCTTATTCCTCTGGCTTTTCTCATGGA
CTTCCAGAATTGTTGAATTGACGTGGCGTCCCTTACTTCTGCTTCCTCAGAGGAATAGGAGCGATGATCC
AGCTATAGGGAACTCTAGGCTTTTGCACAGAGTGTCACCGGAATTGCACCAGGGCAGATGAGCAACTTTT
TCTGTCTAGGTGACATGCTGATTCAGAGGTCCTTCAGTCAGAGCAGCAAGTGATACTTAGCAGCTCTTCC
ATGGTCAGCCATGATGGCAGTGAGGGAGGCCAGGACTGGTTCTTTTGCTGGCTTGCTCTCCTCAACCTGC
CTTCCCTGGGTTTCCATCTCAGTATCCTGTAAATGCATCCACCCTGGTAAACCCTGCTCTATTTTGGGTC
TGGAAGGCATGGACCACATAATGCTGCCCACATGTGGGGAACATAGCCTTCTCGGAGCTTGATTCGATGA
AGTCTCAGCTCCAGAAGGTTCCCGCTGTCCTGGTGGAAGTCACACATGCTGTCCCATGATGACCAGTAAC
CCTGTCCTGCATGGTAAGGTGGGAAGAACACCTTCTCTTGGAGCCTTGAACAGAAACACCGCACGCAGCA
GGCTGGAGCTGGATGGCCCTCCCAGCAGACTCCAGTCCCATTTCCTGGCCAGAAGGAAATGTGCACAGAG
AAGCCACCTTTATGTAGGAGTTTAGAATCTGACCAGCCAACTTCTCTCTCACAGATGGGCGTAGGCTGTG
CTGTGTGGTTTTCCCTTGGGGAGGCGGGAGCAAGAAAAAGTATTTGTAGCTTGTTTTATAAAAAATAAAA
ATGGGTAAACTTGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3673732 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3673732 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003378 same experiment
 EMAGE:29879 same embryo
 EMAGE:32176 same embryo
 EMAGE:29532 same embryo
 EMAGE:29537 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS