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EMAGE:31381

Slc1a5 solute carrier family 1 (neutral amino acid transporter), member 5 ( MGI:105305)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381
euxassay_006886_01 euxassay_006886_02 euxassay_006886_03 euxassay_006886_04 euxassay_006886_05
EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381
euxassay_006886_06 euxassay_006886_07 euxassay_006886_08 euxassay_006886_09 euxassay_006886_10
EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381
euxassay_006886_11 euxassay_006886_12 euxassay_006886_13 euxassay_006886_14 euxassay_006886_15
EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381 EMAGE:31381
euxassay_006886_16 euxassay_006886_17 euxassay_006886_18 euxassay_006886_19 euxassay_006886_20
EMAGE:31381 EMAGE:31381 EMAGE:31381
euxassay_006886_21 euxassay_006886_22 euxassay_006886_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
midbrain meninges
moderate moderate
homogeneousmoderate expression: see section 05 06 07 14 15 16 17 18 weak expression: see section 08 09 10 11 12 13
clavicle
moderate moderate
regionalmoderate expression: see section 08 09 15 16
rectum
moderate moderate
regionalmoderate expression: see section 12
telencephalon meninges
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19 weak expression: see section 08 09 10 11 12 13 20 21 22 23
midgut loop
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 16
bladder
strong strong
regionalstrong expression: see section 11 12 13
esophagus
strong strong
regionalstrong expression: see section 11 13 moderate expression: see section 12
pancreas
weak weak
regionalweak expression: see section 08 09 10
lower jaw molar
moderate moderate
regionalmoderate expression: see section 05 18
upper jaw molar
moderate moderate
regionalmoderate expression: see section 05 18
trachea
moderate moderate
regionalmoderate expression: see section 12
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 13 14
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
spinal cord meninges
moderate moderate
homogeneousmoderate expression: see section 14 15 weak expression: see section 08 09 10 11 12 13
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 16 17 moderate expression: see section 08 09 10 13 14 15
hindgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 18 19 20 21 22 23
mandible
moderate moderate
regionalmoderate expression: see section 05 07 08 09 10 13 14 15 16 17 21 weak expression: see section 02 03 04 06 18 19 20
liver right lobe
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23
heart right ventricle
weak weak
regionalweak expression: see section 13 14 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09
diencephalon meninges
moderate moderate
homogeneousmoderate expression: see section 06 07 14 15 16 17 weak expression: see section 08 09 10 11 12 13
urethra of male
strong strong
regionalstrong expression: see section 11 12 13
midgut
moderate moderate
regionalmoderate expression: see section 14 15
thyroid gland
moderate moderate
regionalmoderate expression: see section 10 14 weak expression: see section 11
hindbrain meninges
moderate moderate
homogeneousmoderate expression: see section 02 03 04 05 06 07 14 15 16 17 18 19 weak expression: see section 08 09 10 11 12 13 20
heart left ventricle
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12
maxilla
moderate moderate
regionalmoderate expression: see section 05 07 08 13 17 weak expression: see section 03 04 06 18 19 20
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 13 14
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 09 10 11 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7371
Entity Detected:Slc1a5, solute carrier family 1 (neutral amino acid transporter), member 5 ( MGI:105305)
Sequence:sense strand is shown

>T7371
CCACGCGTCCGGGACCCTCCAGTTTCCGAGATCCGGTGGACGCATAACTTCAAGGACCGCTGCAAAGTTT
CAGCCTCCATTCTCGGTCCTAAGGACGTCACACCTAGTCTCCAGGCGCACAAGGAACCTCCCTGTTACGG
CTCAGCATGGCAGTGGATCCCCCTAAGGCTGACCCCAAAGGGGTAGTAGCGGTGGATTCCACCGCGAACG
GTGGCCCCGCGCTGGGATCCAGAGAGGACCAGAGTGCGAAAGCAGGTGGTTGCTGCGGTTCCCGTGACCG
GGTGCGCCGCTGCATTCGCGCCAACCTGCTGGTGCTGCTCACGGTGGCTGCGGTGGTGGCTGGCGTGGGG
CTGGGGCTGGGGGTCTCGGCGGCGGGCGGTGCTGACGCGCTGGGTCCCGCGCGCTTGACCGCTTTCGCCT
TCCCGGGAGAGCTGCTGCTGCGTCTGCTGAAGATGATCATCCTGCCGCTCGTGGTGTGCAGCCTGATCGG
AGGTGCAGCCAGCTTGGACCCTAGCGCGCTCGGTCGTGTGGGCGCCTGGGCGCTGCTCTTTTTCCTGGTC
ACCACACTGCTCGCGTCGGCGCTCGGCGTGGGTTTGGCCCTGGCGCTGAAGCCGGGCGCCGCCGTTACCG
CCATCACCTCCATCAACGACTCTGTTGTAGACCCCTGTGCCCGCAGTGCACCAACCAAAGAGGTGCTGGA
TTCCTTTCTAGATCTCGTGAGGAATATTTTCCCCTCCAATCTGGTGTCTGCTGCCTTCCGCTCTTTTGCT
ACCTCATATGAACCCAAAGACAACTCATGTAAAATACCGCAATCCTGTATCCAGCGGGAGATCAATTCAA
CCATGGTCCAGCTTCTCTGTGAGGTGGAGGGAATGAACATCCTGGGCCTGGTGGTCTTCGCTATCGTCTT
TGGTGTGGCTCTGCGGAAGCTGGGGCCCGAGGGTGAGCTGCTCATTCGTTTCTTCAACTCCTTCAATGAT
GCCACCATGGTCCTGGTCTCCTGGATTATGTGGTACGCACCCGTTGGAATCCTGTTCCTGGTGGCCAGCA
AGATTGTGGAGATGAAAGACGTCCGCCAGCTCTTCATCAGCCTCGGCAAATACATTCTGTGCTGCCTGCT
GGGCCACGCCATCCACGGGCTCCTGGTTCTGCCTCTCATCTACTTCCTCTTCACCCGCAAAAATCCCTAT
CGATTCCTGTGGGGCATCATGACACCCCTGGCCACTGCTTTCGGGACCTCTTCTAGCTCTGCCACCTTGC
CTCTGATGATGAAGTGTGTAGAGGAGAAGAATGGTGTGGCCAAACACATCAGCCGGTTCATCCTACCCAT
CGGCGCCACGGTCAACATGGACGGGGCGGCGCTGTTCCAGTGTGTGGCGGCAGTGTTCATCGCACAACTA
AACGGGGTGTCCCTGGACTTCGTGAAGATCATCACCATCCTGGTCACAGCCACAGCATCCAGTGTCGGTG
CGGCAGGGATCCCTGCAGGGGGCGTCCTCACCCTCGCCATCATCCTGGAAGCAGTCAGCCTGCCTGTGAA
GGACATCTCCTTGATCTTGGCCGTGGACTGGCTAGTGGACAGGTCCTGTACTGTCCTCAACGTGGAAGGT
GATGCTTTTGGGGCTGGACTGCTTCAGAGTTACGTGGATCGAACCAAGATGCCGAGCTCAGAGCCCGAAT
TGATCCAGGTGAAGAACGAGGTGTCTCTGAATCCACTGCCCCTCGCCACAGAGGAGGGGAATCCCCTCCT
GAAACAGTACCAGGGACCCACCGGGGACTCCAGTGCCACGTTCGAAAAGGAATCTGTCATGTGAATATCA
GGAGGGACTTTCTACGCCCTGGTGACTTCACGTTGGAAGCCGGGTCTTGCGAGCTGGAGAAATGGACTGG
ATGAGGGCTCTAGGGGTGGGCTGTTTACACACTCCAGGAATCTAGGGGTCGGTGTCTCCTTCCTGGAGAG
GCTATTAGGAGCCTTGTTGCTGGGGTGCATATGCTTATATGTGAATGTGTCCACAAATCCCACCCCCTCT
CCGTTCTGTCCCCCATTCAAGGAATGACATTCTCAGTCACCCAGAGCCATCTGGCCTACCCTCTCAGGAA
ATGGGTTGTCCTGTAGAGTTCTCTACCCTTTTAAAGGCAAAAGTATTGGGATGCAATACTATCTTAATGT
CCCTATCTGTGGTGTGTGTGCACTTGCTGTGACCTTCCCAGTAGTCCCTCCATGACAGAAAACACTCCCA
TGAGTCTCGAGGGAAAGCCAAGAATAAATGTCACTCCAGAACACAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4192790 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4192790 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006886 same experiment
 EMAGE:31440 same embryo
 EMAGE:31477 same embryo
 EMAGE:29414 same embryo
 EMAGE:31203 same embryo
 EMAGE:29525 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS