EMAGE mouse gene expression spatial database

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EMAGE:31440

Nfia nuclear factor I/A ( MGI:108056)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006871_01	euxassay_006871_02	euxassay_006871_03	euxassay_006871_04	euxassay_006871_05

euxassay_006871_06	euxassay_006871_07	euxassay_006871_08	euxassay_006871_09	euxassay_006871_10

euxassay_006871_11	euxassay_006871_12	euxassay_006871_13	euxassay_006871_14	euxassay_006871_15

euxassay_006871_16	euxassay_006871_17	euxassay_006871_18	euxassay_006871_19	euxassay_006871_20

euxassay_006871_21	euxassay_006871_22	euxassay_006871_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

regional

strong expression: see section 10 11 15 16

ventral grey horn

strong

regional

strong expression: see section 09 10 11 12 13 moderate expression: see section 14

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

pons ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 07 08 09 16

rest of cerebellum marginal layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20

pons mantle layer

strong

regional

strong expression: see section 06 07 09 14 15 16

dorsal grey horn

strong

regional

strong expression: see section 11 12 13

spinal cord ventricular layer

strong

regional

strong expression: see section 10 11 12 13

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 09 10 14 15

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 15 16 17

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 moderate expression: see section 07 16 weak expression: see section 17

hypothalamus ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 18 19 20 21 22

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8141
Entity Detected:	Nfia, nuclear factor I/A ( MGI:108056)
Sequence:	sense strand is shown >T8141 CGCGCGGGCAGCTCGCGGGCACCCGGCCGGCCGGCGCGGGAGCGGGAAAGGGTGCGCTATGCCTTTAACG CCCGCGTACAGTAGACAGTATAGTGGAGTGTAGGGAAACTCTAGGCGGGGTTAAAGTTCAGCGCGTGGAG CGGCAAGAGCGCTGGCTGCTGCAGTTGAGCCGAGTTGGAAATGTGAACGCAAGAAGCAGGCTTGATTTTT TTTTCCTCCCCCCCTCCTAGCTCTCTCCTCCTCTCTCTCCCTCTCGCACCCACACTCACGCACACCTCCA GCCCGCACACAGACGTGCACGCACCCCCCGCCGCCCGGCAGTTATGTATTCTCCGCTCTGTCTCACCCAG GATGAGTTTCATCCTTTCATTGAAGCACTTCTGCCCCATGTCCGCGCCTTCGCCTACACATGGTTCAACC TGCAGGCCCGAAAGCGGAAATACTTCAAAAAACATGAGAAGCGCATGTCGAAAGAAGAGGAGAGGGCCGT GAAGGATGAACTGCTAAGCGAGAAGCCCGAGGTCAAGCAGAAGTGGGCTTCCCGACTTCTGGCCAAGTTA CGGAAAGATATCCGACCCGAGTACCGAGAGGATTTTGTTCTTACAGTTACAGGGAAAAAACCTCCATGCT GTGTTCTTTCCAACCCTGATCAGAAAGGCAAGATGCGGAGAATTGACTGCCTCCGCCAGGCAGATAAAGT ATGGAGGTTGGACCTCGTCATGGTGATCTTGTTCAAAGGTATTCCGCTGGAAAGTACTGATGGCGAGCGC CTTGTAAAGAGTCCACAGTGCTCTAATCCAGGGCTCTGTGTCCAGCCCCATCACATAGGGGTTTCTGTAA AGGAACTCGATTTATATTTGGCATACTTTGTACATGCAGCAGATTCAAGTCAATCTGAAAGTCCCAGCCA GCCAAGTGAAGCTGACATTAAGGACCAGCCAGAAAATGGACATTTGGGCTTCCAGGACAGCTTTGTCACA TCAGGTGTTTTCAGTGTGACTGAGCTAGTAAGAGTGTCACAAACACCAATAGCTGCAGGAACCGGCCCCA ATTTTTCTCTCTCTGATTTGGAAAGTTCTTCATACTACAGCATGAGTCCAGGAGCAATGAGGAGGTCTCT GCCCAGCACATCCTCTACCAGCTCTACAAAGCGCCTCAAGTCTGTGGAGGATGAAATGGACAGTCCTGGT GAAGAACCATTTTACACAGGCCAAGGGCGCTCCCCAGGGAGTGGCAGCCAGTCCAGTGGATGGCATGAAG TAGAGCCAGGCTTGCCATCTCCAAGCACACTGAAGAAGTCTGAGAAGTCTGGTTTCAGCAGCCCCTCCCC TTCGCAGACCTCCTCCCTGGGAACAGCATTCACACAGCATCACCGACCTGTCATTACAGGACCCAGAGCA AGTCCACATGCGACGCCATCGACTCTCCACTTTCCAACGTCACCCATCATCCAGCAGCCTGGGCCTTACT TCTCACACCCAGCCATCCGTTACCACCCTCAGGAGACGCTGAAAGAGTTTGTCCAACTTGTCTGTCCTGA TGCTGGTCAGCAAGCTGGACAGGTGGGGTTCCTCAATCCCAATGGAAGCAGTCAAGGCAAGGTGCACAAC CCATTCCTCCCCACCCCAATGTTGCCGCCGCCGCCACCACCACCGATGGCCAGACACCAAGCCTCCAACC ACACCTCACCGACCTACTCGACACCCAGCACCTCCCCCGCAAACCGATTCGTCAGTGTTGGACCACGGGA TCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTACCTGGGATAAAAGTTGCAGCATCCCACC ATCCTCCAGACAGACCACCTGACCCCTTCTCAACTCTGTAACATGGACGCAACCTCAACCCGGCGCAGTT ACAACTTCACTGTCAGTGGAAGGGGAGCGAAACCAACTCGAATCAACTTATACATGGAAACAGCAAACAT CGTGGTCAAACAGCAAAGGCCATAACTTTTTGGGATTCTTTTTTTTTTCTTTTTTTCTTTTTTTCTTTTT TTTTTTCTTTTTTAATACTTTAGGGACTGTCGTAATTTCTCATGGTGCTGGAAATGGTTGGGCTGTGTGA CATTTGAAGTGTTTCAGTGGTGGCGTGAGCAGTAGGTGAAGTGGTTGGTGAAGGATGAGCCTCGCTCGCG AACTTCCTGTGGTAACACTTGGGAGCCTGGCCTTTTCCCAGTATTTCCTGAATTTGCCCGTGCAGGTGTG TGACCCTCCCTGAGCATCTGGAGACACCTGGAGCATGAAACCTTCTGTAATCGCTGAGATCTGTAACTTA CAGGACACTCAAAGGGCAATGGTTTTTTGGGGGGGGGGGGTTGTTTTTTTGGTTTTTTTTTTTTCTGTAA CATATTAGGGAAAAAATGCAGTTAAGTTGATCTTTCTTTTAGTTTGGTTTGGTTCAGCAGTCAGCAGTTA CATACATAACACAACCCGCAAGGACAGTGAATCCGCTCCTGTTGCAGAGCAATTTCCAAAAAGACGGCAA AGACTACTACTATCAGTTTGGTTGGGTTTTTGTTTTTTTGTTTTTTTGTTTTTTTGTTTTTTTTGTTTTT TTTGTTCTTAAGTTTTGAAATGCTGCACTTACATTTAAAAAAAAAAACATTTTTCAACAATTTCAACAAT GACACAAAAATTCACATGGAAATGGGGAAGATGGTCTGTTTTGACAGAAACTGACAGGAATCAAAACAAT CGAATTTTGAATTGAGTGAAGTGCAATTTCATTGGATAGCTAAGTATCTTTGTAAGATAGAGATTGTTGA AAATTCTATTTTTGTTTTTCAAGTCCTCCCACCCCAGGACTCTAAATTATTGGGGTAAAAAACAGCCTTG CAAGAAAAAGGGGAGCTATTTTTGCTTTTTATGTTTTTTATTGTTAAACTTGTATCCCTTTAAAAAAAAA CCGAAGGAAAATTTTTTAAAGGGATACAAGTTTAACAATAAAAAACATAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30087137 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30087137 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006871 same experiment
	EMAGE:31381 same embryo
	EMAGE:31477 same embryo
	EMAGE:29414 same embryo
	EMAGE:31203 same embryo
	EMAGE:29525 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source