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EMAGE:31457

Ndrg1 N-myc downstream regulated gene 1 ( MGI:1341799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457
euxassay_004423_01 euxassay_004423_02 euxassay_004423_03 euxassay_004423_04 euxassay_004423_05
EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457
euxassay_004423_06 euxassay_004423_07 euxassay_004423_08 euxassay_004423_09 euxassay_004423_10
EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457
euxassay_004423_11 euxassay_004423_12 euxassay_004423_13 euxassay_004423_14 euxassay_004423_15
EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457
euxassay_004423_16 euxassay_004423_17 euxassay_004423_18 euxassay_004423_19 euxassay_004423_20
EMAGE:31457 EMAGE:31457 EMAGE:31457 EMAGE:31457
euxassay_004423_21 euxassay_004423_22 euxassay_004423_23 euxassay_004423_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
mandible
moderate moderate
regionalmoderate expression: see section 09 10 11 17 18 19 20
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
midbrain meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
palatal shelf
strong strong
regionalstrong expression: see section 13 14
diencephalon meninges
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
rectum
strong strong
regionalstrong expression: see section 13
telencephalon meninges
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
anterior naris
strong strong
regionalstrong expression: see section 13 moderate expression: see section 14 15 16
stomach pyloric region
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12
midgut
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20
hindbrain meninges
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 moderate expression: see section 07 09 10 11 15 16 17 18 19
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
external naris
moderate moderate
regionalmoderate expression: see section 12
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 12 16 17 moderate expression: see section 10 11 18 19
naris
moderate moderate
regionalmoderate expression: see section 15
upper jaw
strong strong
regionalstrong expression: see section 11 17 moderate expression: see section 09 10 18
nasal septum
strong strong
regionalstrong expression: see section 13 14 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7576
Entity Detected:Ndrg1, N-myc downstream regulated gene 1 ( MGI:1341799)
Sequence:sense strand is shown

>T7576
CCCACGCGTCCGCCCACGCGTCCGAGGCTACCCTCTCGCGTTGCAGGCAGATTGCTCACCCATCTTCGTC
TCCCTCTTCACCTCCCGCGGGGCGCTGCACCTCCAACCTTGTGGTTGGCTCCTTTCCGTGCTTGCTTGCT
CATTAGGTGTGTGATAGCACGGAAATGTCCCGAGAGCTACATGACGTGGACCTCGCTGAGGTGAAGCCTC
TGGTGGAAAAAGGGGAGAGCATCACTGGTCTCCTGCAAGAGTTCGATGTTCAGGAGCAGGATATTGAGAC
CCTACATGGCTCCCTGCATGTCACCCTGTGTGGGACCCCCAAGGGCAACCGTCCTGTCATCCTCACGTAT
CACGACATCGGCATGAACCACAAGACCTGCTACAACCCCCTCTTCAACTCTGAGGACATGCAGGAGATCA
CACAACATTTTGCTGTCTGCCATGTGGATGCCCCTGGCCAACAGGATGGTGCCCCTTCCTTCCCAGTGGG
GTACATGTACCCCTCAATGGATCAGTTGGCTGAAATGCTTCCTGGAGTCCTTCACCAGTTTGGGCTCAAG
AGTGTCATTGGCATGGGGACAGGAGCTGGCGCCTACATCCTGACCCGCTTCGCACTCAACAACCCTGAGA
TGGTAGAGGGTCTCGTGCTCATGAATGTGAACCCCTGTGCTGAAGGCTGGATGGACTGGGCTGCCTCTAA
GATCTCAGGATGGACCCAAGCCCTGCCTGACATGGTGGTGTCCCACCTCTTCGGCAAGGAGGAGATACAC
AACAACGTGGAGGTAGTGCACACGTACCGCCAGCACATCCTCAACGACATGAACCCGAGCAACCTACACT
TATTCATCAGCGCCTACAACAGCCGGAGAGACCTGGAGATTGAGCGGCCAATGCCTGGAACCCACACTGT
CACTTTGCAGTGCCCTGCTCTGCTGGTAGTTGGAGACAACTCTCCTGCCGTGGATGCTGTGGTGGAATGC
AATTCTAAATTGGACCCAACGAAGACCACCCTGCTCAAGATGGCAGACTGTGGAGGCCTTCCTCAGATCT
CTCAGCCCGCCAAGCTTGCTGAGGCCTTCAAGTACTTTGTGCAGGGCATGGGATACATGCCTTCTGCCAG
CATGACTCGCCTGATGCGGTCCCGCACAGCCTCTGGCTCCAGTGTCACATCCCTGGAGGGCACCCGTAGC
CGCTCCCATACCAGCGAGGGCCCCCGAAGCCGCTCGCACACCAGTGAGGGGTCCCGTAGCCGCTCCCATA
CCAGTGAGGATGCCCGTCTCAACATCACCCCCAACTCGGGTGCCACCGGAAACAATGCCGGGCCCAAGTC
CATGGAGGTGTCCTGCTAAGCAATCTGTGCATCAGTCACCCTGGACTGGTGGCCTCTCCCTTAGCCCCCA
ACCCTTGCCTGCCACCCTACGCTAATGCGGTATTAATCTAAAGCTGCTTTTGTAAGAATGAACTCTGGCG
GTGGCAGACCCAGGCTGTTCTGACTGCCTCCTGGGACCAGGGTGGGTGGTGGTGGACCAAAGGCAAGAAG
CAGTTCAGCGTACTGTTCTGCTAACAAGTGGCCTGGTGGACCCTCTCTCTACCCTCAGACACCAAACTGC
CAAAACTAAGAAATAGATAGCTGTAACACTGCCTGGCTCCAAGCCCAGGCTGATCCAGCATTCTGGTTCA
TAGGCGTTTTGCCAACCTGCCTACTTCCTGTTTCCTCCCCACTAAACTCATTCCTGGAAACAAACTTCTG
TTTTGAAAGGAGAGAGAGAGGCAGGAAAGTTGGGCGCCTTATTTTAAGTAGAGAAACCAAGAAAGGAAGA
GAAAAAAAGGCAGGGTCTAGGGCAGGTAGAGGAAAAACACAACAAGTCTACTAGGCTCACTTGAAGGAAT
TGATTTTAAAGTTCTGGACACTGAAACCCACAACCTGTTTTTCTGGCAACCTTTTAACGTGCAAGAAAAA
CTGGTAACAAACCCCAGGCTTTATAGAGAAAGTGATCCAGCAAATGTGGGGCATCAGTGATTTAAGACTC
TTGCTATGCAGTCTACTGTACCCCGCTCCCATTCCACAGTGAATGAGTGGATGACACCTCTGTCCCTCGG
GAAGATGGTTTTGATTTGTTGTTGCATTCTTTGAGGCAGAGGGAGAACCCAGCAGGTGACAGGGGTCCTT
GTCTGGGATGAGTCTTTAGGATGGGAAGGGTGGGGATAGAATGGCCCTGGGATTTTGAGGACTCATGGTA
GGGCCAAGTGCTAGAACCTGGTGGGTGTGATTTCATTGATCTGGAATCTTGTGTGACTGAGTCACACCTT
GTCTCCCATAATTGATGTCTGGCATTGATTTGAAACTTGATAGCTGAATGTCTCTTAACTGGCCCATCGC
TGGTTCTTCAGAGAGAACCGTATGTTCTAGTGGGGGAAGGACTGGTTTTGGGTTCTGTACATTCCACGGG
AACTTGCTTCCCTCTCCTGCCCCTCCTGCTGACTGGGTTTGTAGCATTGGCCTCTTAAGCAATGCCAGCC
TAATGCCCATGCTCCATACTACTGCCTTTTGATTCTTACTGTGGCTAACCTTGAACCTTTCCTATAGGAA
ATGTGATCAGATCAGAGACACTTGATCGTTGGGAGTCAGCCCTCAAGGCCTGGCGACTTCCAGGATCTTG
GAGTTGCTAGAGGAAAAGTAGTTATGCCCTATAGGCGGCATGAGAGACAAGGGATCAGTTTACCTGCCAA
CTCCTACCGTGGAGGAGGAGTTCCACTGACCTTGTGTAGAAAGATCAGGAATCCAGGCCAGGGTGATTGA
ATGCTGACAAGAAATCACTGTATATGTCTCTCCAGGGCAGTTTCTTAATAAGATGTTTGTATTTATTTCC
AGACCAATAAATTTGTAACTTTGCAGTGAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4242075 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242075 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004423 same experiment
 EMAGE:31417 same embryo
 EMAGE:30372 same embryo
 EMAGE:30371 same embryo
 EMAGE:31481 same embryo
 EMAGE:31403 same embryo
 EMAGE:31402 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS