EMAGE mouse gene expression spatial database

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EMAGE:31481

Dab2 disabled homolog 2 (Drosophila) ( MGI:109175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004432_01	euxassay_004432_02	euxassay_004432_03	euxassay_004432_04	euxassay_004432_05

euxassay_004432_06	euxassay_004432_07	euxassay_004432_08	euxassay_004432_09	euxassay_004432_10

euxassay_004432_11	euxassay_004432_12	euxassay_004432_13	euxassay_004432_14	euxassay_004432_15

euxassay_004432_16	euxassay_004432_17	euxassay_004432_18	euxassay_004432_19	euxassay_004432_20

euxassay_004432_21	euxassay_004432_22	euxassay_004432_23	euxassay_004432_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord meninges

moderate

regional

moderate expression: see section 11 12 13 14 15 16

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7575
Entity Detected:	Dab2, disabled homolog 2 (Drosophila) ( MGI:109175)
Sequence:	sense strand is shown >T7575 CAGAGATAGAATCAAGCTTGCAAGAGATTTGTTGAAGAGAGAAAAGTCAAGGTGTACTTTGTGGGTTCTG TCCTTTTGAGACCTGCCCGTCGGGGATTGGCTGGTATCAGTGACTGTCTACTGCTGGATTTTCTGCTTGC CTTCCCGTCATGTCTAACGAAGTAGAAACAAGCACAACCAATGGCCAGCCTGACCAACAGGCTGCCCCGA AAGCGCCATCAAAGAAGGAGAAGAAGAAAGGTTCTGAAAAGACAGACGAGTACTTGTTGGCCAGGTTCAA AGGTGATGGTGTAAAATACAAGGCCAAGCTAATCGGTATTGATGATGTGCCTGATGCTCGAGGAGACAAA ATGAGTCAGGATTCTATGATGAAACTCAAGGGAATGGCAGCAGCTGGTCGCTCTCAGGGACAACACAAGC AAAGAATCTGGGTCAACATTTCCTTGTCTGGCATAAAAATCATTGATGAGAAAACTGGGGTAATTGAACA TGAACATCCAGTAAATAAGATTTCCTTCATTGCTCGTGATGTGACAGACAACAGAGCATTTGGTTATGTG TGTGGAGGTGAAGGCCAGCATCAATTTTTTGCTATAAAAACAGGGCAACAGGCTGAACCATTAGTCGTCG ATCTTAAAGACCTTTTTCAAGTTATCTATAATGTAAAGAAAAAGGAAGAAGATAAGAAAAAGGTTGAAGA AGCCAACAAAGCAGAAGAGAATGGAAGTGAGGCCCTAATGACCCTTGATGATCAAGCTAACAAATTGAAG CTGGGTGTTGACCAGATGGATTTGTTTGGGGACATGTCTACACCTCCTGACCTAAATAGTCCAACATCTT CAGCAAACGACTTGCTTGCTTCAGACATCTTTGCCTCAGAACCTCCAGGCCAGATGTCCCCCACAGGACA ACCTGCAGTCCCGCAGTCGAACTTTCTGGATCTCTTCAAAGGCAATGCTCCTGCCCCAGTGGGGCCCCTT GTAGGTCTAGGTACGGTCCCAGTAACACCCCCCCAAGCAGGACCCTGGACGCCTGTTGTCTACAGTCCTT CGACAACTGTGGTCCCAGGAGCCATAATAAGTGGCCAGCCTCCCAGTTTTGGCCAGCCACTCGTTTTTGG TACAACCCCAGCAGTACAAGTCTGGAATCAGTCTCCATCATTTGCAACCCCAGCTTCCCCTCCACCCCCC ACAGTTTGGTGTCCTACCACATCTGTGGCGCCCAACGCTTGGTCATCCACAAGCCCTCTGGGGAATCCTT TTCAGAGTAATAATATCTTTCCACCTCCCACCATGTCCACTCAGTCCTCTCCTCAGCCTATGATGTCCTC TGTTCTGGCCACACCGCCTCAACCACCTCCCCGAAATGGCCCACTAAAGGACATTCCCAGTGACGCTTTC ACTGGCTTAGACCCCCTTGGGGATAAAGAGGTCAAGGAAGTGAAAGAAATGTTTAAGGACTTCCAGCTGC GGCAGCCACCTCTTGTTCCCTCAAGGAAGGGGGAGACGCCTCCCTCTGGGACTTCAAGCGCCTTCTCCAG TTACTTCAACAATAAAGTTGGCATTCCTCAGGAGCATGTAGACCATGATGATTTTGATGCCAATCAACTG TTGAACAAGATTAATGAACCACCAAAGCCAGCCCCGAGACAAGGTGTCCTCTTGGGTACCAAGTCTGCTG ACAATTCACTCGAGAACCCTTTCTCCAAAGGGTTCAGCTCATCAAACCCCTCTGTGGTTTCTCAGCCTGC ATCTTCTGATCCCCACAGGAGCCCTTTCGGAAATCCTTTTGCCTAGCTTCTGAAGTTGTAATGATGACTA TCCAGATGAGCAAAAGACTGGCTTTGGTCAAGAATGAAGCAGACAGCCAGAAACATGTTGACCTCTGTCC TCGCTCCAGCTTTGACGTATTATCTGTTACCCTATTTGTTTTGGCCTCTTGTACTTGTAAAATGCCTTTC ATTTTCCTGTCTAGGCTAAAGCTAAACTTAAACTATGGCTTTACGTAAATTAAGCTCCTAAACTCTCTAG CTCCAATATAAATGAAGTAGCTTCCCTATCAAATCCTTGTCTGTTGTGCCCCCTTGAAACTTCCAGAATA TTCTCCATTCTACCCTCCATTTGGGAGGAGCGGCTACCTTTACCCTTAATATCACACTGCCTTGAGTCAA TGTCCAAATACTCATAGCTCTCAAAGTCATTTGGGGTTCCTGGTGTGCGGCCTAAACCTAAAGCATCCTA TTAATAGGGAAGTAAGACACCTTGCTTCCTATGTCCACTCAGAAAGAATTTTATTTAATAAAATGAAAGC AAGACTAACTTCTCAAATCCACCCAAGGACCATTTTGAGATGGTCGTTTCTCAGCTAACTGCACCATTTA CCAATCCTGCCCCAAGTGGTGCTTACATTTGACTTGAAGAAGAGAAAGAGCTAACTCAAAACACAAGGCA TTATTCAAAGCTAATAAAACAATTTCTCCCTGGGGCCCCACATTGTTTTCATTCCAGATACGTTGCAGCT GTTTGACCCTGATGACATTATGCCCTACATTTTCCTTGAAGATCCTGATTTTATTTCATGTGATTTTTGT TTCTCAATAAAGATGATTATTGTGTGCATTACTAAAAAATAATAAAGTATAATTTCTCTCATTCTGGTGA TCTCTCCCAGGACCTAGGCTGTTCCATTTTCAATCTCTTGCTGGCTGTTTTAAATGAGAGGAAAGCATTC TTTATCTCTCCCTGTCTTTCTCTCTTTCTCTTTCAGTTCATTTTGCTCCTGGGTTTATATAAACAGTGCC TCCCTTTTACCAGGAATGGATTTTTCACGGGGCAAAAAGCATTAAATGTTTCACAGATCTGAAGTGCACG CACCCACTCCAGTCATTGTTAGTCCAGGGGCTAGACTTCTGGAAGGTGCACCTTCTTCATTCTCCCTATG GCTTGGGGGACTTAGACTGAAGTTTCATAGAGCAATTTAACACAGTAGGGGAACTGGATTCTGCTCAGCA GTGAAGGACTGTATTCTGAAAGCTAAATGTGACTATGCTAGACACACTGGGTCCTTACTGGATGACTAGG CCTTTATCAAGTAACTGGAAAACTTTCTTGAAGCCACAATCATGGACAAATATTTGGGGTTATCATTGAA AAATCTGCCTGAAATATATTCCTCATAGCCTTGGAAACCTACACAGTACATTTTCATTTCCAGGCTTTTT GTTATACAGAGCTGAAGGGTTTGTTCCTGCCAAGTTGTGTTTTTTTTTTTAGTTTATGTTGAGTATTTTT TTTTCAATGTTATCTCTTGACCCAAAATGCTCAGGTCCTTGTGGGTGTTAGTCCAATCTTACCTTTTGTT GGAAGAGAATGACTGCTCAGGTTTTAAACAAGGCGCTAGCCTTGTGAGCCTCTGTTAATTAGCCCCAAGT GATCCAAGCTGAAGTCCTGTGGGTTTCTGTTTAATGCTAGTGGTTAATTATCTATGATATGTGTTTCTTT TTTCCTGTCTGACTTCCTACTCAGTCATTATGAACAGACTTGAAATCATACTTTAAAAATTTCAAATACC TGAAGATGTGCTAAAGTGAAGGTAACTCTTTCTAGATAGTCGAGTTTTTGAAAGTAATGATCAGCCACAC AACTGTTTTGTATGTACTTGTCTTCTTGTGCACTTTTCTGTATGCAAACAAATCTATAAATTTACGCATT CAATAAACTGGAATGACAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242174 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242174 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004432 same experiment
	EMAGE:31417 same embryo
	EMAGE:30372 same embryo
	EMAGE:30371 same embryo
	EMAGE:31403 same embryo
	EMAGE:31457 same embryo
	EMAGE:31402 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source