EMAGE mouse gene expression spatial database

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EMAGE:31417

Slc22a2 ( MGI:1335072)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004445_01	euxassay_004445_02	euxassay_004445_03	euxassay_004445_04	euxassay_004445_05

euxassay_004445_06	euxassay_004445_07	euxassay_004445_08	euxassay_004445_09	euxassay_004445_10

euxassay_004445_11	euxassay_004445_12	euxassay_004445_13	euxassay_004445_14	euxassay_004445_15

euxassay_004445_16	euxassay_004445_17	euxassay_004445_18	euxassay_004445_19	euxassay_004445_20

euxassay_004445_21	euxassay_004445_22	euxassay_004445_23	euxassay_004445_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord meninges

moderate

regional

moderate expression: see section 10

midbrain meninges

strong

regional

strong expression: see section 04 05 19 20 21 moderate expression: see section 06 07 08 09 10 17 18

diencephalon meninges

strong

regional

strong expression: see section 19 20 21 moderate expression: see section 06 07 08 09 10 17 18

telencephalon meninges

strong

regional

strong expression: see section 04 05 19 20 21 22 23 moderate expression: see section 06 07 08 09 10

hindbrain meninges

strong

regional

strong expression: see section 04 05 11 12 13 14 15 16 19 20 21 22 moderate expression: see section 03 06 07 08 09 10 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7581
Entity Detected:	Slc22a2, ( MGI:1335072)
Sequence:	sense strand is shown >T7581 ATCAGCTGCCTCCAGGAACCATGCCGACCGTGGATGATATTCTAGAACATATAGGGGAATTCCACCTTTT CCAGAAACAAACATTTTTCCTCTTAGCTCTGCTCTCCGGTGCCTTCACCCCCATCTATGTGGGCATCGTT TTCCTGGGCTTCACCCCCAACCACCACTGCCGGAGTCCTGGAGTGGCCGAGCTGAGCCAGCGGTGTGGCT GGAGCCCAGCAGAGGAGCTGAACTACACCGTGCCGGGCCTGGGGTCTGCGGGTGAGGTCTCCTTCCTCAG CCAGTGCATGAGGTATGAGGTGGACTGGAACCAGAGCACCCTTGACTGTGTGGACCCACTGTCCAGCCTG GCTGCCAACAGGAGCCACTTGCCACTGAGCCCCTGCGAGCATGGCTGGGTGTACGACACTCCCGGCTCCT CCATCGTCACTGAGTTTAACCTAGTGTGTGCTCACTCCTGGATGCTGGACCTGTTTCAGTCATTAGTGAA CGTGGGGTTTTTCATCGGTGCTGTGGGCATTGGTTACCTAGCAGACAGGTTTGGGCGGAAGTTCTGCCTC TTGGTCACCATCCTCATCAATGCTATCTCTGGGGTTCTCATGGCGATTTCACCCAACTATGCCTGGATGT TGGTGTTTCGATTTCTACAGGGACTGGTCAGCAAAGCGGGCTGGTTAATTGGCTATATCCTGATTACAGA ATTTGTTGGGCTGGGCTATCGCAGAACAGTAGGGATTTGTTACCAAATCGCCTTTACTGTTGGGCTCCTG ATCCTGGCTGGAGTGGCCTATGCCCTTCCCAACTGGAGGTGGCTGCAATTTGCCGTGACTCTGCCCAACT TCTGCTTCCTGCTCTATTTCTGGTGCATACCGGAGTCTCCAAGATGGTTGATCTCCCAGAACAAAAATGC AAAAGCCATGAAAATCATTAAGCATATTGCTAAGAAAAATGGAAAATCGGTGCCAGTCTCTCTTCAGAGC CTGACGGCAGATGAGGATACTGGCATGAAATTGAACCCTTCGTTCCTGGACTTGGTCAGAACCCCTCAGA TAAGGAAACATACGTTGATCTTGATGTACAATTGGTTCACGAGCTCTGTTCTCTACCAGGGCCTCATCAT GCACATGGGCCTCGCAGGGGACAACATCTACTTGGATTTCTTCTACTCTGCCCTGGTGGAATTCCCAGCT GCCTTCATCATCATTCTCACCATAGACCGGATTGGTCGCCGCTATCCCTGGGCTGTGTCAAATATGGTGG CAGGAGCAGCCTGCCTAGCTTCGGTTTTTATCCCTGATGATCTACAGTGGCTGAAAATTACCGTGGCGTG CTTGGGTAGAATGGGCATCACCATAGCCTACGAAATGGTCTGCCTGGTCAACGCTGAGCTGTACCCCACA TACATCAGGAATCTTGCTGTCCTTGTCTGCTCCTCTATGTGTGACATTGGTGGCATCGTCACACCTTTCC TGGTCTACCGTCTCACTGACATCTGGCTGGAGTTCCCGCTGGTCGTATTTGCTGTGGTTGGCCTTGTTGC TGGGGGACTTGTGCTATTGCTACCTGAGACCAAAGGGAAGGCTCTGCCTGAGACCATCGAGGATGCTGAG AAGATGCAGAGGCCAAGAAAAAAGAAAGAAAAGAGAATTTACCTCCAAGTTAAGAAAGCAGAACTAAGCT AAAGAGAAAGAGCATCATTGCTGCTGGAGATGACTTTGCTCTCTCTGAGACCAGAGGCAGAGCTTCTCTT CCCCTCCCGCGAAAGCCACACAACCCAACCTCACTTACCCCTGAATTCCTGAACTCCATCAGCAGTGAGC TGTCGCCCGCCCAGTTTGTTGCACTGATGTGTCCAGCTCTTCCTCTCAACCAGGACCTCACTCCACCTCA CTCCACTCTCAACAGTCCTGGGGAATGCCCATTGCTTTGCTGGATTTGTTGGGGTTTTTTTCATCTGTAC ATTTCTTTACTTGGTTTCTTTCTCCAAGCATGACATCAAACCAAAATATACAAGGGAGCTGTGGGTCAGG ACAACTAACTATTAAAAAAAAATCTGAAAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242038 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242038 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004445 same experiment
	EMAGE:30372 same embryo
	EMAGE:30371 same embryo
	EMAGE:31481 same embryo
	EMAGE:31403 same embryo
	EMAGE:31457 same embryo
	EMAGE:31402 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source