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EMAGE:31510

1810046J19Rik ( MGI:1913678)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31510 EMAGE:31510 EMAGE:31510 EMAGE:31510 EMAGE:31510
euxassay_001703_01 euxassay_001703_02 euxassay_001703_03 euxassay_001703_04 euxassay_001703_05
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euxassay_001703_21 euxassay_001703_22 euxassay_001703_23 euxassay_001703_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 13 14 15 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 15 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20
telencephalon marginal layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
ventral grey horn
moderate moderate
regionalmoderate expression: see section 08 09 11 12 13
olfactory cortex marginal layer
moderate moderate
regionalmoderate expression: see section 11 12 13 15 16 17 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 18
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 17 18
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2722
Entity Detected:1810046J19Rik, ( MGI:1913678)
Sequence:sense strand is shown

>T2722
CGGGCATTGAGATTGAGTCGCGACTCGGGGGGACAGGGGCCTTCGAGATTGAGATCAATGGACAGCTGGT
GTTCTCCAAGCTGGAGAATGGAGGGTTTCCTTATGAGAAAGACCTCATGGAAGCCATCCGAAGAGCCAGC
AATGGAGAACCTGTAGAAAAGATCACCAACAGCCGGCCTCCCTGTGTCATCCTGTGACTAAGAAATGATT
CTTGGAAGCTTGGTTCTGGGGTTTAAACCTTTCCTTGTGGTTACGCTAGGAATCCCTCTACCTTTAGCCC
CTCACTTGGCCCTAGGTAACTAAGACCCTGACTTCACTTTGCCTCTTAGGGCACAAGGAGGGACTAGACA
TTTCTGTGGCCAAAGGCAGAAAAGAGATGTTCTCTGTGAACATCACCATCGCTCTCCCGGGATCTGTGCC
CTCTGCAGTTTGGTCCTCTCCCCAGCGTGGCAATACCTTTCAGATGCCACGGTTGCTATAACTTATTTTC
CCTGAATCCTGGGCAGTGTCGGCTCTTGGCAATAAAGAGTAATTACAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1513041 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513041 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001703 same experiment
 EMAGE:29570 same embryo
 EMAGE:29740 same embryo
 EMAGE:31525 same embryo
 EMAGE:31425 same embryo
 EMAGE:29569 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS