EMAGE mouse gene expression spatial database

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EMAGE:31553

Mcm5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) ( MGI:103197)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006125_01	euxassay_006125_02	euxassay_006125_03	euxassay_006125_04	euxassay_006125_05

euxassay_006125_06	euxassay_006125_07	euxassay_006125_08	euxassay_006125_09	euxassay_006125_10

euxassay_006125_11	euxassay_006125_12	euxassay_006125_13	euxassay_006125_14	euxassay_006125_15

euxassay_006125_16	euxassay_006125_17	euxassay_006125_18

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 10 11 12 13 14 moderate expression: see section 09

hypothalamus ventricular layer

strong

regional

strong expression: see section 09 13 moderate expression: see section 10 11 12

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 14 15 16

ventral grey horn

weak

regional

weak expression: see section 06 07 08

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

rest of cerebellum marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 11 12 13 14 moderate expression: see section 07 08

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 01 02 04 05 06 10 11 13 14

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 09 13 moderate expression: see section 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8530
Entity Detected:	Mcm5, minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) ( MGI:103197)
Sequence:	sense strand is shown >T8530 GTGGCGCGCCGCTGTGAGGTACAGTCATGTCGGGCTTCGACGACCCGGGCATTTTCTACAGCGACAGCTT CGGTGGCGACCCCGGTGCGGAAGAGGGCCAGGCCCGCAAGTCGCAACTGCAGAGGCGATTCAAGGAGTTC CTGAGACAGTACCGAGTGGGCACCGATCGCACGGGCTTCACCTTCAAGTACAGAGATGAACTCAAGCGGC ATTACAACCTGGGTGAATACTGGATCGAGGTGGAGATGGAGGACCTGGCCAGTTTTGACGAGGAACTGGC TGACCACTTGCATAAACAGCCGGCCGAGCACTTACAGCTGCTTGAGGAAGCTGCCAAGGAGGTGGCAGAT GAGGTGACCCGGCCCCGGCCAGCTGGAGATGAGCTGCTCCAAGACATCCAGGTCATGCTCAAGTCAGATG CCAGCCCGTCGAGCATTCGGATTCTGAAGTCAGACATGATGTCACACCTGGTGAAGATCCCTGGCATCAT CATTTCAGCCTCTGCAGTCCGTGCCAAGGCTACTCGTATCTCCATTCAGTGCCGCAGCTGCCACAATACC CTCACCAATATCGCCATGCGCCCAGGCCTAGAGGGCTATGCCCTTCCCAGGAAGTGCAATATGGATCAGG CTGGGCGCCCAAAGTGCCCACTGGATCCATACTTCATCATGCCTGACAAGTGCAAGTGTGTGGACTTCCA GACTCTGAAACTGCAGGAGCTGCCTGATGCAGTCCCTCATGGTGAGATGCCCAGGCACATGCAGCTTTAT TGTGACAGGTACCTGTGTGACAAGGTTGTTCCTGGGAACAGGGTCACCATCATGGGCATTTATTCCATCA AGAAGTTTGGCTTGAACCCCAGCAAGGGCCGGGACAGGGTAGGTGTGGGCATCCGGAGCTCGTACATCCG AGTGCTGGGCATCCAGGTGGACACAGATGGCTCTGGCCGAAGCTTTGCTGGGTCTGTCAGCCCACAGGAA GAGGAGGAATTTCGTCGCCTGGCTGCCCTCCCCAACATATATGAGCTCATCTCCAAGAGCATTTCCCCCT CCATCTTTGGGGGCATGGATATGAAGAAGGCCATTGCCTGCCTGCTTTTTGGGGGTTCCCGGAAGAGGCT CCCAGATGGACTCACTCGCCGAGGTGATATCAACTTGCTGATGTTGGGAGACCCTGGTACAGCCAAGTCT CAGCTTCTGAAGTTTGTGGAGAAGTGCTCTCCCATTGGGGTGTACACATCTGGGAAAGGTAGCAGTGCTG CTGGCTTGACTGCCTCAGTGATACGGGACCCCTCATCTCGAAACTTCATCATGGAAGGTGGAGCCATGGT TCTGGCCGATGGTGGGGTTGTCTGTATTGATGAGTTTGACAAGATGCGGGAAGATGACCGTGTTGCAATC CATGAGGCTATGGAGCAGCAGACCATCTCCATTGCTAAAGCTGGGATCACTACCACCTTGAACTCTCGCT GCTCTGTTCTGGCTGCAGCCAACTCAGTGTTTGGCCGATGGGATGAGACAAAAGGGGAGGACAATATTGA CTTCATGCCTACCATCTTGTCCCGATTTGATATGATCTTCATCGTCAAAGATGAGCACAATGAGGAGAGG GACATGATGCTAGCCAAACATGTGATGACTCTGCATGTGAGTGCACTGACACAGACACAGGCTGTGGAGG GTGAGATCGACCTGGCCAAGATGAAGAAGTTCATTGCCTACTGCCGAGCGAGGTGTGGACCTCGGCTATC AGCAGAGGCAGCAGAGAAGCTGAAAAACCGCTACATCATCATGCGGAGTGGGGCTCGTCAGCATGAGAGG GACAGTGACCGGCGTTCCAGCATCCCCATCACTGTGCGGCAGCTGGAGGCTATTGTGCGCATTGCTGAGG CCCTCAGTAAGATGAAACTGCAGCCCTTTGCCACTGAGGCTGATGTAGAGGAGGCATTGAGACTGTTCCA GGTGTCCACACTGGATGCTGCTTTGTCTGGCAATCTGTCGGGGGTGGAGGGCTTCACTACCCAGGAGGAC CAGGAGATGCTGAGCCGCATTGAGAAGCAACTCAAGCGCCGTTTTGCCATTGGCTCTCAGGTGTCTGAAC ACAGCATTGTCCAGGACTTCACCAAACAGAAATATCCAGAGCACGCTATCCGAAAGGTGCTGCAGCTCAT GCTACGCAGGGGTGAGATCCAACACCGTATGCAGCGCAAGGTGCTCTATCGCCTCAAGTGAGCCCATTGC CCATCAACCCTCAAGCCTGAAATGCTGCCACCACCCTATCTCCCAGTCAGTGCTCCAAACCTCCTTTTGC CCTGCCTCTCCACCTCAGACTGCAGTCTGCAGCACATCTGCAGCCCCTGGAAATGTACTTTGGTCTGTTG GCCTCATACTGTGTTTGAGTGTCTGAGGACTCTCTGCTCTGGGTGTCTATCCCCTGTCATGCCTTCTCAA CAAGATGAGTCTGGAGCAGGAACAGGCCCTGGAATGTAGATGGGTCTGTATATTGGCTCCCGGGCCACTC ACTGCCAAGCTTCTTTGTATGTACAGAGGTAATAAAGCAATTGAGTCCCTGGCTGCTAAGGTCAGTGGAC CCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5707724 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5707724 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006125 same experiment
	EMAGE:29604 same embryo
	EMAGE:29591 same embryo
	EMAGE:31563 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source