EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31563

Glra2 ( MGI:95748)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006123_01	euxassay_006123_02	euxassay_006123_03	euxassay_006123_04	euxassay_006123_05

euxassay_006123_06	euxassay_006123_07	euxassay_006123_08	euxassay_006123_09	euxassay_006123_10

euxassay_006123_11	euxassay_006123_12	euxassay_006123_13	euxassay_006123_14	euxassay_006123_15

euxassay_006123_16	euxassay_006123_17	euxassay_006123_18	euxassay_006123_19	euxassay_006123_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8540
Entity Detected:	Glra2, ( MGI:95748)
Sequence:	sense strand is shown >T8540 GGAAACTAGATTCCTGCCAGATTTTGAGTCTGGACAATAAATAGGCACTTTGTCCTAGTCTCCTTTCTGG AATCATTTCGGGATATTTTCCACAACCATCACAGAAACAGGAATGTACCGGCAGCTAGTGAACATTTTGA CAGCCTTGTTTGCATTTTTCTTAGGGACAAACCACTTCAGGGAAGCTTTCTGCAAAGACCATGACTCCAG GTCTGGAAAACATCCCTCGCAGACCCTATCTCCTTCAGATTTCTTGGATAAATTAATGGGAAGAACATCA GGATATGATGCAAGAATCAGGCCAAATTTTAAAGGTCCTCCAGTAAACGTTACTTGCAATATTTTTATCA ACAGTTTTGGATCAGTCACAGAAACCACCATGGACTACCGAGTGAACATTTTTCTGAGACAGCAGTGGAA TGATTCACGGCTGGCATACAGTGAGTACCCAGATGATTCCCTGGATTTGGATCCCTCAATGTTGGATTCG ATTTGGAAACCGGATTTGTTCTTTGCCAATGAAAAGGGTGCCAATTTCCATGATGTCACCACTGACAACA AGTTGTTGCGGATTTCCAAAAATGGCAAAGTGCTCTACAGTATTAGACTCACCTTGACTTTATCTTGTCC AATGGACCTGAAGAACTTTCCAATGGATGTCCAGACCTGTACAATGCAGCTGGAGAGTTTTGGGTACACC ATGAATGACCTGATATTTGAGTGGTTAAGTGATGGTCCAGTACAAGTTGCTGAAGGACTCACCTTGCCCC AGTTTATTTTGAAAGAAGAGAAGGAGCTTGGTTATTGCACAAAGCATTACAACACTGGCAAGTTTACCTG CATTGAGGTCAAGTTTCACCTGGAGCGCCAGATGGGCTACTATTTGATCCAGATGTACATCCCCAGCCTG TTGATAGTCATTTTGTCCTGGGTCTCCTTTTGGATAAACATGGATGCAGCCCCTGCCAGGGTTGCCCTGG GCATCACAACAGTCCTGACAATGACTACACAGAGTTCAGGTTCCAGGGCATCTCTGCCAAAGGTCTCCTA TGTGAAAGCAATTGACATCTGGATGGCGGTATGCCTTCTCTTTGTGTTTGCTGCCTTACTGGAATATGCA GCAGTGAACTTTGTCTCCAGGCAACATAAGGAGTTCCTTCGTCTCCGGAGACGACAGAAGAGGCAGAATA AGGAAGAAGATGTTACTCGTGAAAGTCGTTTTAACTTCAGTGGCTATGGGATGGGTCACTGCCTCCAAAT GAAAGATGGCACAGCTGTCAAGGCTACACCTGCCAACCCACTTCCACAACCCCCCAAAGATGCAGATGCT ATCAAGAAGAAGTTTGTGGATCGGGCAAAAAGAATTGACACCATATCTCGAGCTGCCTTCCCACTGGCCT TCCTCATTTTCAACATCTTTTACTGGATCACATACAAGATCATTCGGCATGAAGATGTCCACAAGAAATA GATGTACCCTGTTGACCCTGGACCTTCTTGCCTAAGTGTTGTGCTTGTAAATACACAGTAATAGTGTCTT TATATCGCTTCAACAGAGAAGAACATTGAGGGTGGGGGGAGGGAAATCACGAGGGTGGGGTTTCTGGCAC CTATATGACAAAAGACAGGTTCTATGGGAAATTAAGAAAACTGCACAAAATTAAGGTGTTGCAGAATCAC ATGAGCATAACTCCCATCCATAGTCTATAGCATTGTTCTTTCAGATGATGCATCGCTCAGACATGATGTG CAAGGTCAAGTTCTCAATGGATGATTAATTTATGGTTTGACTTGATGAGATTTGGTTCTGAAAGTTTAGT TTAATGCGCGCGAGTACGCGCGTGCGTGCGCGCACACACACACACACACACACACACACACACACACACT GAAAATGCTTACCATCTGACAATAGTGACTAGTCTATAGTAAATAATTGAGGACCAAACTTTTTCAGGAA AATGCTGCCTCGTTTTTAAAACAAGCCTCCTGAGCTATGACATTTACAATGTCTGTAATTAGTGTTTCAC CCAAGAAATCCTTCTGTGGGTTGTAAATTCTTTCTATGTACCAGAAAACAAGATAAACACAAATGACAAA GAACAGATTTCTACTCTACTGTCTGTAGGTGTCCACTTTGATATTATTTTTCTTTCCTAGATGTAAATTG GGGGTTTATTGTGTGTTGCGTAATTGATTTACTGGTATATCCTCTTAAAACAAATGCTCATTTTATTTTA GATCAAGTTAGCATACTATGTATATTTTTTTCACTTTGGCTATATTTACGTGTGACTTTAAGTGCCCAAG TGTAACTTAGCTATTGCTGTCGCTAAGCCACAGTATTTATGGAGATGGTGTGTTGTGACCGGTGTAGCTC AGATTAGCTCAAACTTCCCATTTCTGCCCTCATTGTAGCTGTAACTACTAGTCCTAATGTTGACTGACCA GTAATTTGTACTTTCAATCCAAGTGAATATCGTTTTAAATATCCTTGACTAACTTAAAAACTTTAAAACT GTACTGTGATTTTCATAACCCGTTGCCTTTTTGGTACCAGAGCTACGTGGTTTGAATCCTGGCTACATGT TTTAAGTAAGAAAAAAAAATGTATTTTTGCTTACTCAAAAGACAACCTGTAAAAATATATGGAAATAATT ACATTTTACATGTGCTGTGAAAGGGATTATTTTAAAAAAGCATTTGTTCAGTTTCAATAAAGATAAGTGT GCCACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708257 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708257 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006123 same experiment
	EMAGE:29604 same embryo
	EMAGE:29591 same embryo
	EMAGE:31553 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source