EMAGE mouse gene expression spatial database

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EMAGE:31588

Msh6 ( MGI:1343961)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006580_01	euxassay_006580_02	euxassay_006580_03	euxassay_006580_04	euxassay_006580_05

euxassay_006580_06	euxassay_006580_07	euxassay_006580_08	euxassay_006580_09	euxassay_006580_10

euxassay_006580_11	euxassay_006580_12	euxassay_006580_13	euxassay_006580_14	euxassay_006580_15

euxassay_006580_16	euxassay_006580_17	euxassay_006580_18	euxassay_006580_19	euxassay_006580_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7945
Entity Detected:	Msh6, ( MGI:1343961)
Sequence:	sense strand is shown >T7945 GCGATCCTTGTAGGCGCTCGGAGCCGTGGCCCGGTCTGCCCGTCAGCCTTCATCATGTCCCGACAAAGCA CCTTGTACAGCTTCTTCCCCAAGTCCCCGGCACTCGGCGACACCAAGAAGGCCGCGGCCGAGGCCTCACG CCAAGGTGCCGCTGCCTCTGGAGCCTCCGCTTCCCGCGGCGGGGATGCGGCCTGGAGCGAGGCTGAACCC GGGTCCCGGTCCGCGGCGGTATCCGCCTCGTCGCCGGAGGCAAAGGATCTCAACGGAGGGCTGCGGAGGG CCTCCTCCTCCGCGCAGGCCGTGCCTCCCAGTTCTTGTGACTTCTCACCAGGTGATTTGGTTTGGGCTAA GATGGAAGGTTACCCCTGGTGGCCTTGCCTAGTTTATAATCATCCCTTTGATGGAACGTTCATCCGGAAG AAAGGGAAATCTGTCCGTGTTCATGTACAGTTCTTTGATGACAGCCCAACAAGGGGCTGGGTTAGCAAAA GGATGTTAAAGCCATATACAGGTTCAAAGTCAAAGGAAGCCCAAAAGGGAGGTCATTTCTACAGTTCAAA GTCTGAGATACTCAGAGCAATGCAACGTGCAGATGAGGCCTTAAGTAAAGACACGGCTGAGAGGCTGCAG CTGGCAGTGTGTGATGAGCCTTCAGAGCCGGAGGAGGAGGAGGAGACAGAGGTGCATGAGGCTTATTTAT CTGATAAGAGTGAAGAGGATAATTACAACGAGAGTGAAGAAGAAGCACAGCCCAGTGTTCAGGGGCCTAG GCGAAGCAGCCGGCAAGTCAAGAAGCGACGGGTCATCTCAGACTCGGAGAGTGACATTGGTGGCTCTGAT GTAGAATTCAAGCCAGACACTAAGCAGGAAGGAAGCAGTGATGACGCGAGCAGTGGAGTTGGGGACAGCG ATAGTGAGGACCTAGGCACCTTTGGCAAAGGCGCTCCAAAGCGGAAGAGAGCCATGGTTGCTCAGGGTGG ACTTAGAAGGAAAAGTCTGAAGAAGGAAACAGGCTCAGCCAAACGAGCAACTCCCATTCTCTCAGAAACC AAGAGTACCTTGAGCGCTTTTTCTGCCCCTCAGAATTCTGAATCCCAGACCCACGTCAGTGGAGGAGGTA ACGACAGTAGTGGACCCACTGTTTGGTATCACGAAACTTTAGAATGGCTTAAGCCAGAAAAGAGAAGAGA TGAGCACAGGAGACGCCCCGATCACCCTGAATTTAACCCCACCACACTGTATGTGCCTGAAGAGTTCCTC AATTCTTGTACCCCAGGGATGAGGAAGTGGTGGCAGCTCAAGTCTCAGAACTTTGACCTCGTCATCTTTT ATAAGGTGGGGAAGTTTTATGAGTTGTATCACATGGATGCTGTTATTGGAGTCAGTGAGCTGGGGCTGAT ATTCATGAAGGGCAACTGGGCCCATTCTGGTTTTCCAGAGATTGCGTTTGGCCGGTTCTCGGATTCCTTG GTGCAGAAGGGCTATAAGGTAGCACGAGTGGAGCAGACCGAGACTCCAGAAATGATGGAGGCGCGATGTC GGAAAATGGCACACGTGTCCAAGTTTGATAGAGTGGTGAGAAGGGAGATTTGCAGGATCATTACCAAGGG CACACAGACCTACAGTGTGCTGGATGGTGATCCCTCTGAGAACTACAGTAGGTATCTTCTTAGCCTCAAA GAGAAGGAGGAAGAGACTTCTGGTCACACTCGTGTGTATGGTGTTTGCTTTGTGGACACTTCACTGGGCA AGTTTTTCATCGGCCAGTTTTCGGATGACCGCCACTGTTCCAGATTTAGGACGCTAGTGGCTCACTATCC TCCAGTACAAATTTTGTTCGAGAAAGGAAATCTCTCAACAGAGACCAAAACTGTCCTAAAGGGTTCACTG TCATCTTGTCTTCAGGAAGGTCTCATACCAGGTTCCCAATTTTGGGATGCCACTAAGACCTTGCGAACAC TCCTTGAAGGAGGGTACTTTACTGGAAATGGAGACAGTAGTACAGTGCTGCCTCTGGTGCTTAAAGGAAT GACCTCGGAGTCAGATTCTGTTGGGTTGACACCAGGCGAGGAGAGTGAATTGGCTCTCTCTGCTCTAGGT GGTATTGTTTTCTACCTCAAAAAATGCCTGATTGATCAGGAGCTTCTGTCAATGGCTAATTTTGAAGAGT ATTTCCCTTTGGATTCTGACACGGTCAGCACAGTAAAACCTGGGGCTGTCTTTACTAAAGCCAGTCAGCG GATGGTGCTAGACGCAGTGACATTAAACAACCTGGAGATTTTCCTGAATGGGACAAATGGCTCTACTGAA GGGACCCTGCTAGAGAGATTGGATACTTGCCATACGCCCTTTGGCAAGCGGCTCTTAAAACAGTGGCTCT GTGCCCCCCTCTGCAGCCCTTCTGCCATCAGTGACCGTCTAGATGCGGTGGAAGATCTGATGGCCGTGCC TGACAAGGTCACTGAAGTTGCAGACCTCTTAAAGAAGCTGCCAGACCTTGAGAGGCTACTGAGTAAGATT CATAATGTTGGGTCTCCCCTGAAGAGCCAGAACCATCCAGACAGCAGGGCTATAATGTACGAAGAAACGA CATACAGCAAAAAGAAGATCATTGATTTTCTCTCTGCTCTAGAAGGATTCAAAGTAATGTGTAAAGTCTC AGGGCTTCTGGAGGAGGTCGCGGGTGGTTTTACGTCCAAAACCCTCAAGCAGGTTGTTACTCTGCAGTCA AAAAGTCCTAAAGGCCGCTTTCCTGACCTGACTGCAGAACTGCAGCGATGGGACACAGCCTTTGACCATG AGAAGGCTCGGAAGACCGGACTCATCACTCCAAAAGCAGGGTTTGACTCTGATTATGACCAAGCACTTGC TGACATCAGAGAGAATGAACAGAGCCTCCTGGAGTACTTAGACAAACAGCGCAGTCGGCTTGGCTGTAAG AGCATAGTCTACTGGGGAATCGGTAGGAACCGTTACCAGTTAGAGATTCCAGAGAATTTCGCTACCCGTA ATCTACCTGAAGAATATGAGCTGAAATCTACTAAAAAAGGCTGTAAACGATACTGGACCAAAACGATTGA GAAGAAATTAGCTAATCTTATTAATGCTGAAGAACGCAGGGACACATCCTTAAAGGACTGCATGCGGCGC TTGTTCTGTAACTTCGACAAGAACCACAAGGACTGGCAGTCTGCTGTAGAGTGCATTGCAGTGTTGGATG TCTTACTGTGCCTGGCTAACTACAGTCAAGGAGGAGATGGTCCTATGTGTCGCCCAGAAATTGTGTTACC AGGAGAAGACACACATCCCTTCTTAGAGTTTAAAGGGTCACGACATCCCTGCATTACAAAGACTTTTTTT GGAGACGATTTTATTCCTAACGACATTCTAATAGGCTGCGAGGAAGAAGCAGAGGAACATGGCAAAGCCT ATTGTGTGCTTGTGACTGGACCAAATATGGGGGGCAAGTCTACACTCATAAGACAGGCTGGTCTGTTGGC TGTGATGGCCCAGCTGGGTTGTTACGTACCTGCTGAGAAGTGCAGGCTCACACCAGTTGACAGAGTGTTT ACTAGGCTTGGTGCCTCAGATAGAATAATGTCAGGAGAAAGCACATTTTTTGTTGAGTTGAGTGAAACTG CTAGCATACTCAGGCATGCAACAGCACATTCTTTGGTACTTGTGGATGAATTAGGAAGAGGCACCGCAAC TTTTGATGGGACAGCGATAGCCAATGCGGTTGTTAAAGAACTTGCTGAAACCATAAAGTGTCGAACACTG TTTTCTACACACTATCATTCATTAGTAGAAGATTATTCTAAAAGTGTTTGTGTGCGCCTAGGACATATGG CATGCATGGTAGAAAATGAATGTGAGGACCCCAGCCAGGAGACTATTACTTTCCTCTATAAATTCATTAA GGGAGCTTGTCCTAAGAGCTATGGCTTTAATGCAGCAAGGCTTGCTAATCTCCCGGAGGAGGTTATTCAG AAGGGACACAGAAAGGCAAGAGAATTTGAGAGGATGAATCAGTCATTACAGCTATTTCGGGAAGTTTGTC TGGCTACTGAAAAGCCGACTATAAACGGTGAAGCTATCCATAGGCTGCTGGCCTTGATTAACGGATTGTA GACTACATTGGAAGCTGAGCTGACTTTTGAAAAAGGTGGTAAATTCAGACAACATTATGATCTAATAAAC TTTATTTTTAAAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6518577 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6518577 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006580 same experiment
	EMAGE:29617 same embryo
	EMAGE:31592 same embryo
	EMAGE:30333 same embryo
	EMAGE:31635 same embryo
	EMAGE:29601 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source