EMAGE mouse gene expression spatial database

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EMAGE:31738

H2-M3 ( MGI:95915)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003704_01	euxassay_003704_02	euxassay_003704_03	euxassay_003704_04	euxassay_003704_05

euxassay_003704_06	euxassay_003704_07	euxassay_003704_08	euxassay_003704_09	euxassay_003704_10

euxassay_003704_11	euxassay_003704_12	euxassay_003704_13	euxassay_003704_14	euxassay_003704_15

euxassay_003704_16	euxassay_003704_17	euxassay_003704_18	euxassay_003704_19	euxassay_003704_20

euxassay_003704_21	euxassay_003704_22	euxassay_003704_23	euxassay_003704_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 13 15 weak expression: see section 14 16

right lung

strong

regional

strong expression: see section 18 19 20 21 22 23 moderate expression: see section 12 15 16 17 24 weak expression: see section 14

left lung

strong

regional

strong expression: see section 05 06 07 08 09 moderate expression: see section 04 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7037
Entity Detected:	H2-M3, ( MGI:95915)
Sequence:	sense strand is shown >T7037 CAGGGCTACCACGGGAAGGATGGGGTCCTCAAGCAACTGCGCTCTCCTGCACATGGTGGTGGTCTCTTTG GCTGTTACCCAAACAGGTTCTGGCTCACATTCACTGCGCTATTTCCACACTGCGGTGTCACGGCCGGGCC GTGGGGAGCCCCAGTATATCTCTGTGGGCTATGTTGACGACATGCAGTTTCAGCGCTGTGATAGCATTGA GGAAATTCCGAGGATGGAGCCTCGTGCACCTTGGATGGAGAAGGAAAGACCAGAGTACTGGAAGGAGCTG AAACTCAAGGTCAAGAACATTGCACAAAGTGCCAGAGCAAACCTTCGGACCCTGCTCCGCTACTACAACC AGAGTGAAGGCGGCTCTCACATCCTGCAGTGGATGGTTTCCTGTGAGGTAGGGCCAGACATGCGCCTTCT TGGAGCCCACTATCAGGCTGCGTATGATGGCTCAGATTACATCACCCTGAATGAAGACCTGAGCTCCTGG ACTGCAGTGGACATGGTGTCTCAGATCACGAAGAGCCGTTTAGAGTCAGCTGGCACAGCAGAGTACTTCA GGGCCTATGTGGAGGGAGAGTGCCTGGAGCTGCTCCACAGATTCCTGCGGAATGGCAAGGAGATTCTTCA GCGAGCAGATCCCCCAAAGGCACATGTGGCCCATCACCCGAGACCCAAAGGTGATGTTACCTTGAGGTGC TGGGCTCTGGGCTTCTACCCTGCTGACATCACCCTGACCTGGCAGAAGGATGAAGAGGACCTGACCCAGG ACATGGAGCTTGTGGAAACGAGGCCTTCAGGGGATGGAACCTTCCAGAAGTGGGCAGCTGTGGTGGTGCC TTCTGGAGAGGAGCAGAGATACACATGTTATGTGCACCATGAGGGGCTAACCGAGCCCCTTGCCCTGAAA TGGGGAAGATCGTCTCAATCATCTGTCGTCATCATGGTCATCGTTGCTAGTCTGGTTCTCCTGGGAGGTG TGATCACTATTGTTGTGGTGTGCAAGAGGAGGGGTGCAGGGGAAAGATGAATCAGTTATACTCAGACTGC CAGCAGGAACAGTGATTAAGTTCTGATGCTTCTTTCACCATTTAGAAAGTTGGCATCTCATGGTGTGTCT CCTGTCAGCACAGTGTGACAAAGTGAGCAGCCCAGGGCATCCCTGAGGACTGAGTCTCCTCTCCATACCT GTGTCTCCTCCTTTTCTCACCTGTACCACAGGGGGATCCAGGACTTCTTCATCCCTTGTGGAACTTTAGG TTTCTATGGGCAGCTTCTTCCCTATTGAAAAATAAGGATGTAGATATAGCTTTATTATTATTATTATTAT TTTACCTTCTCGATAAAAGCTGTACTTCATGCTGTTAAAAGTGTAAGATTTGTACATTGGAAAGGAAATA AAACATGAAAACTCACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4007538 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4007538 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003704 same experiment
	EMAGE:31171 same embryo
	EMAGE:31087 same embryo
	EMAGE:31217 same embryo
	EMAGE:31214 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source