EMAGE mouse gene expression spatial database

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EMAGE:31930

Tspan1 ( MGI:1914055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004539_01	euxassay_004539_02	euxassay_004539_03	euxassay_004539_04	euxassay_004539_05

euxassay_004539_06	euxassay_004539_07	euxassay_004539_08	euxassay_004539_09	euxassay_004539_10

euxassay_004539_11	euxassay_004539_12	euxassay_004539_13	euxassay_004539_14	euxassay_004539_15

euxassay_004539_16	euxassay_004539_17	euxassay_004539_18	euxassay_004539_19	euxassay_004539_20

euxassay_004539_21	euxassay_004539_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

stomach

moderate

regional

moderate expression: see section 01 05 weak expression: see section 02 03 04

rectum

moderate

regional

moderate expression: see section 08

midgut loop

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5778
Entity Detected:	Tspan1, ( MGI:1914055)
Sequence:	sense strand is shown >T5778 CCTGGAGCTGTTCTGTTCCTTCTGGAGTGCAACAGATCCCGTTCAGAACCTATTGCCAAGAGCCCTGAAG CAGGACACACCATGCAGTGCTTCAAATTCATTAAGGTCATGATGTTCCTCTTCAATCTACTCATCTTTCT CTGTGGTGCAGCCCTGTTGGCTGTGGGAATCTGGGTGTCCGTCGATGGGACATCTTTCCTGAAGGTCTTC GGATCACTATCATCCAGTGCCATGCAGTTTGTCAACGTGGGCTACTTCCTCATCGCCGCTGGTGCTGTGC TCTTCATTCTTGGTTTCCTGGGCTGCTATGGTGCTCACTCTGAGAACAAGTGTGTGCTCATGATGTTCTT TTCCATCCTCCTCATCATCTTCATCGCTGAGATTGCAGGTGCTGTGGTTGCTTTGGTGTACACCACATTG GCTGAACAATTCCTGACACTCCTGGTGGTGCCTGCTATCGAAAAAGACTATGGTTACCAGACTGATTTCA CCCAAGTATGGAACACTACAATGGAAGAGTTGCATTGCTGTGGCTTTAACAACTACACAGATTTTAATGC CTCACGTTTCGTCAAAGAGAATAAAGTCTTCCCCCCACCCTGTTGTGCCAACCCTGGCAACCATACAGTT GAACCATGCACCGAGGAGAAGGCCAAAAGTATGAAAGTACAGGGTTGTTTCAAAGAGATTCTGCATAGAA TCAGAGCCAATGCAGTCACTGTGGGTGGTGTGGCAGTTGGAGTTGCGGCCCTAGAGCTGGCTGCCATGGT TGTATCCATGTATCTATACTGCAATCTGAAATAAGACTACTACTTCCTCCTGACTTGCTGCCGTCACATG GAAACCAGGAAGAGGCACTTGGCACAGCTAAGCAGCAGCAATGGGTGGAGATGAAATGTCATTTGGGCTG GAATGGATTTGCCTTTGCTGCTCAGGGACTTTGGGCTAAATAGGGACCACCTCGTGGGCTTTGTCTGCCT TGTTCTTCCACTGGTGGGGTGAGGGGGGCATCGGTCCCAGTCTCAGAGGCAGCCAAGTCTGCCCATTTCC TGAGACTCTTGATGATCCTAGCACTCCACTAGTGGATGAAGGCACTTACAGCCTGGGCATTTTCTAACAC TGTAAAGTCATTGAAGGAATGTATAGAAGGCATTTTGGAACCTATAAACCAATGAAAATATTTCTAATTG ACCTGTAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2654254 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2654254 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004539 same experiment
	EMAGE:31937 same embryo
	EMAGE:30645 same embryo
	EMAGE:31932 same embryo
	EMAGE:30643 same embryo
	EMAGE:30631 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source