EMAGE mouse gene expression spatial database

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EMAGE:32049

Il1rap ( MGI:104975)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003867_01	euxassay_003867_02	euxassay_003867_03	euxassay_003867_04	euxassay_003867_05

euxassay_003867_06	euxassay_003867_07	euxassay_003867_08	euxassay_003867_09	euxassay_003867_10

euxassay_003867_11	euxassay_003867_12	euxassay_003867_13	euxassay_003867_14	euxassay_003867_15

euxassay_003867_16	euxassay_003867_17	euxassay_003867_18	euxassay_003867_19	euxassay_003867_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

homogeneous

strong expression: see section 15 weak expression: see section 08 09 10 11 12 13 14 16 17 18 19

ventral grey horn

moderate

single cell

moderate expression: see section 09 10 11 12 13 14 15

kidney calyx

moderate

regional

moderate expression: see section 08 18

4th ventricle lateral recess

weak

homogeneous

weak expression: see section 03 04 05 06 07 09 10 11 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7239
Entity Detected:	Il1rap, ( MGI:104975)
Sequence:	sense strand is shown >T7239 CCACGCGTCCGGGCTGCCAGCATCTGGCTTTCCTAGGTTCGGCTTGGACCAGGGCGCTGAAAGAGGCAGT GGTCGGCCACCCTGCATCCATCTGGTCGCTGTCGCCGAGCCTGCTGGGCTGGCCCGATAAGGATGGGACT TCTGTGGTATTTGATGAGTCTGTCCTTCTATGGGATCCTGCAGAGTCATGCTTCGGAGCGCTGTGATGAC TGGGGACTAGATACCATGCGACAAATCCAAGTGTTTGAAGATGAGCCGGCTCGAATCAAGTGCCCCCTCT TTGAACACTTCCTGAAGTACAACTACAGCACTGCCCATTCCTCTGGCCTTACCCTGATCTGGTACTGGAC CAGGCAAGACCGGGACCTGGAGGAGCCCATTAACTTCCGCCTCCCAGAGAATCGCATCAGTAAGGAGAAA GATGTGCTCTGGTTCCGGCCCACCCTCCTCAATGACACGGGCAATTACACCTGCATGTTGAGGAACACAA CTTACTGCAGCAAAGTTGCATTTCCCCTGGAAGTTGTTCAGAAGGACAGCTGTTTCAATTCTGCCATGAG ATTCCCAGTGCACAAGATGTATATTGAACATGGCATTCATAAGATCACATGTCCAAATGTAGACGGATAC TTTCCTTCCAGTGTCAAACCATCGGTCACTTGGTATAAGGGTTGTACTGAAATAGTGGACTTTCATAATG TACTACCCGAGGGCATGAACTTGAGCTTTTTCATCCCCTTGGTTTCAAATAACGGAAATTACACATGTGT GGTTACATATCCTGAAAACGGACGTCTCTTTCACCTCACCAGGACTGTGACTGTAAAGGTGGTGGGCTCA CCAAAGGATGCATTGCCACCCCAGATCTATTCTCCAAATGACCGTGTTGTCTATGAGAAAGAACCAGGAG AGGAACTGGTTATTCCCTGCAAAGTCTATTTCAGTTTCATTATGGACTCCCACAATGAGGTCTGGTGGAC CATTGATGGAAAGAAGCCTGATGACGTCACAGTCGACATCACTATTAATGAAAGTGTAAGTTATTCTTCA ACGGAAGATGAAACAAGGACTCAGATTTTGAGCATCAAGAAAGTCACCCCGGAGGATCTCAGGCGCAACT ATGTCTGTCATGCTCGAAATACCAAAGGGGAAGCTGAGCAGGCTGCCAAGGTGAAACAGAAAGGTAATGG ATGCACCGAGCCGATGACTCTGTGAACTCTCACGGTAACACAGTGGTGAATAGGAAATAAAGGAGAGAAT TTCTGCACCCACATCGGACACAAAACACTGAGTCAGTTAAATGAAAAATCCTAAGAGAAAGCTTCCATCT TTGTGATAATGATCAGTATGCTGGTTCCGATGTTGTCAACGCCTTTGTTTCTAAATGTTGCTTGAAGAAA ATCCATGAATACATGGGTACAATAAGACACAATTTCATATCTTAGATAATCATGAAAAATGAAAATATTC CAAGTGAGTAAAGATGTAACAGAATTGCTAGAGCCACATATGGTCAGTGAAGCACAAAGATGCCTTTAGA AGGAACTAGAAACAGGGTTCGAGAGTCATAAGAGTGATTGTGCCACATGACCTCATCCTTTGGGAATTTT TCCTGAATTATGCACCCACAGCTGTTCTTAGAAGTATATAAAAGACTAAAATGTAACTAGCAGACATCTA TTTGTGTAGAAGAAGTTAAGGAGGGCAGAGAATATAAATATTAATTAATATTAATTCTCAGAAAATTTCA AATTCACATTTTAAACAAGTACCAGTGTTTAGGCAGTTTTATAATGTTATAATTGATAAAATAAAACTTA TTAAAATATGATTTCATTTGCTGTTCAAATAATTTCTCCATTAACATGTGTATTTTCTATAATAAAGGAA AATTACAAATCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4161899 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4161899 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003867 same experiment
	EMAGE:29826 same embryo
	EMAGE:32051 same embryo
	EMAGE:29830 same embryo
	EMAGE:32059 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source