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EMAGE:32098

Aqp1 aquaporin 1 ( MGI:103201)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098
euxassay_002187_01 euxassay_002187_02 euxassay_002187_03 euxassay_002187_04 euxassay_002187_05
EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098
euxassay_002187_06 euxassay_002187_07 euxassay_002187_08 euxassay_002187_09 euxassay_002187_10
EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098
euxassay_002187_11 euxassay_002187_12 euxassay_002187_13 euxassay_002187_14 euxassay_002187_15
EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098
euxassay_002187_16 euxassay_002187_17 euxassay_002187_18 euxassay_002187_19 euxassay_002187_20
EMAGE:32098 EMAGE:32098 EMAGE:32098 EMAGE:32098
euxassay_002187_21 euxassay_002187_22 euxassay_002187_23 euxassay_002187_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
humerus
weak weak
regionalweak expression: see section 01 02 03 18 19 20
clavicle
weak weak
regionalweak expression: see section 11 12 14 16
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 16 17 weak expression: see section 06 07 08 09 15
male reproductive system
weak weak
regionalweak expression: see section 13
axial skeleton
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 13 14 weak expression: see section 12
viscerocranium
strong strong
regionalstrong expression: see section 08 09 10 11 16 17 18 19
metencephalon part of 4th ventricle choroid plexus
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18
hindlimb digit 3 mesenchyme
moderate moderate
regionalmoderate expression: see section 24
vibrissa
moderate moderate
regionalmoderate expression: see section 05 21 22 23 weak expression: see section 06 07 08
upper lip
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 10 11 12 13 14 15 17 18
heart valve
weak weak
regionalweak expression: see section 09 11 12
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 06 07 08 09 10 16 17 18
nasal septum
strong strong
regionalstrong expression: see section 12 13 14 15 16 17
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 04 05 06 07 17 18 19 20 21 22
lower lip
moderate moderate
regionalmoderate expression: see section 16 17 weak expression: see section 09 10 11 13 14
mandible
weak weak
regionalweak expression: see section 04 05 06 07 08 09 16 17 18 19 20
thyroid cartilage
weak weak
regionalweak expression: see section 09 10 11 12
vault of skull
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 03 04 05 06 07 17 18 19 20 21 23
tongue
moderate moderate
regionalmoderate expression: see section 13
rib
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 13 weak expression: see section 01 02 03 14 15 16 17 18 19 20 21 22 23 24
foot mesenchyme
moderate moderate
regionalmoderate expression: see section 05 06 23 24
hindlimb digit 2 mesenchyme
moderate moderate
regionalmoderate expression: see section 24
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 13 14 15 16 17
hand mesenchyme
moderate moderate
regionalmoderate expression: see section 23 24
liver lobe
moderate moderate
regionalmoderate expression: see section 01 05 09 10 13 16 17 18 19 20 21 weak expression: see section 02 03 04 06 07 08 11 12 14 15 22 23 24
exoccipital bone
strong strong
regionalstrong expression: see section 02 moderate expression: see section 17 18 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6071
Entity Detected:Aqp1, aquaporin 1 ( MGI:103201)
Sequence:sense strand is shown

>T6071
GTTGAGCACCAGGCATCCAGCGGTCAAGTCGAGGCCCCTGCCAGCATGGCCAGTGAAATCAAGAAGAAGC
TCTTCTGGAGGGCTGTGGTGGCTGAGTTCCTGGCCATGACCCTCTTCGTCTTCATCAGCATCGGTTCTGC
CCTAGGCTTCAATTACCCACTGGAGAGAAACCAGACGCTGGTCCAGGACAACGTGAAGGTGTCGCTGGCC
TTTGGTTTGAGCATCGCTACTCTGGCCCAAAGTGTGGGTCACATCAGCGGTGCTCACCTCAACCCTGCGG
TCACACTGGGGCTCCTGCTCAGCTGTCAGATCAGCATCCTCCGGGCTGTCATGTACATCATCGCCCAGTG
TGTGGGAGCCATCGTCGCCACGGCCATTCTCTCGGGCATCACCTCCTCCCTAGTCGACAATTCACTTGGC
CGCAATGACCTGGCTCACGGTGTGAACTCCGGCCAAGGCCTGGGCATTGAGATCATTGGCACTCTGCAGC
TGGTACTGTGCGTTCTGGCCACCACTGACCGGAGGCGCCGAGACTTAGGTGGCTCAGCCCCGCTTGCCAT
TGGCTTGTCTGTGGCCCTTGGACACCTGCTGGCGATTGACTACACTGGCTGCAGTATCAACCCTGCCCGG
TCATTTGGCTCTGCTGTGCTCACCCGCAACTTCTCAAACCACTGGATTTTCTGGGTGGGGCCGTTCATTG
GGGGTGCCCTGGCAGTGCTCATCTATGACTTCATCCTGGCCCCACGCAGCAGCGACTTCACAGACCGCAT
GAAGGTGTGGACCAGTGGCCAGGTGGAGGAGTATGACCTGGATGCTGACGACATCAACTCCAGGGTGGAG
ATGAAGCCCAAATAGAGGAGGCTTGGCCTGGGCATCTATATGGGGGTGGGGCAGGGATGGGCGGAGGGAG
GGGAGGGCCGAAATCATATTGTAGACACTCTGACAAGCTGACCAAAGTCACTCCCCAAGACCTGCCTGAC
CCACATGGTCTAGCCTTGTCTGGGGCAGTAATATCACTGTCTTTCTTTCTGTTTCCTGGTCTCAGAGCTT
CCTGGGGACCAAGATTTATCAACTCAGCACCCCACTCCTTTGACATCATGGAGGAGTGAAAGCGAGGGAC
CCACTTGCTAGTTGTCCCCTCAGAGTGTGCTAGGAAGTCCCCCTCATTCCAAAGCTGCCCAGCAAGTCCC
CCACCTTAGGTGCCTGGGATTCTGCCAATGTCGCTTTGTGCCTTTGACCGCAGCCTTCCTTGGGGTAGGC
ACCTTATCTCCAAAGGCACTTTGAGGGAGAGGAGGTCCCCTAACTTTCCCCTTTGGTCTGACTTACCTCC
AGGACCCTTCCCCTTGAACTCACTCTAAGACCTTGGAATTGTCCCTGTGCTGAGGCCTCAGTGATCACAT
CTGTAAAGTGGCAAGGAAGGGACAGCTTTGGGGATTCTGTAAGTGTGGACATGCCACAGGTTCTAGAAGG
GACAGTCTTGACAGCACTGCTTTGTGTACCCGGCCTATACTCTTTCCCAGCGTCCACAGCCCACGAGCAC
ACGTGGATGGGCCACGGATCAGAGAATCAGTGTGGGATGCAAGACGTGCCTGTTCACACACACAGCCTTC
CTAGAGGCGGGTTAGCTGTAGCAGAACGAAGGTCATTTTGTGGTAGAAGAAGAGCTCACACTATCTTACC
TAGCTCTAGTGGGTCTGCCCGTGTAACATGGCACCGACTGGATGTTTATTTCGGTCCTCCAGTCTCCCTT
GCAGTATAGATCTGGTGTGTCTGTGTTTGTTAAAGAGCATTGGGCTATGTGCAGTGTCATGTCTGAGGAC
ATGCTGGGCACACAAGGGTTTGGATGCCCTTAGTTCATCATGAGCAAGGAGGTCACTCTGGCCCTGGCTT
GTGAGCTCCTGGCTGGATGAGTGTGACTGGAGCCTGGACAATCTGCAGAGGCTTACTCTGTGCCTCCAGG
CACAGTCTTCTCTCTGCATTGGCCCAATGCTACAGCTTATGTTGCAGCCCAAGGACAGCTCAGAGTGCAG
TCAGCTCAGGGGTTGCATTTAGCTCTAGGTCATTCCATTGGATCCCATCAATCCAGAGTGGCTCTACCAC
TGTGCCCTTAACCATGTTGTGAACCGAGAGCCACATTCTTCAGGTGCTTAGAAGCAGCAGAAGAGTCAGG
AGGCCACTGACCACTGGCATATCACAGCTGCACACTCGTCTCCGGTCCCTAGCAGACACTGTACCCACTT
AACAGATAAGGGCACTGAGGACCCATATGCTCAACTGTATCATATTGGATCAAGCTTAGCAGTCAAGGCC
ATGTTTGGCCACCCCATGGTCAATGACGTGGACTCCTCTCACCTCCAGCTTCTCAGTTTCTGCCTGGGCA
ATGGCTAAGGGCCAGGGGTGAGGGTGGGGGTGGAGGTGGTGAGAGCTGTGCTGGAGGGGGGCAGGGCAGG
CCCATCCCACCTGGTTCTGGCCATTGTGAACCAGGACCCTGTATCTGTCTGCTCTGTGTATGTTTCTTTG
CAATTGAATTTCATCTTATGGTAAGAAAATAAAGGACAATGACTTGTAAGGTCAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3491276 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3491276 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002187 same experiment
 EMAGE:32240 same embryo
 EMAGE:32243 same embryo
 EMAGE:29866 same embryo
 EMAGE:29635 same embryo
 EMAGE:30114 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS