EMAGE mouse gene expression spatial database

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EMAGE:32111

Dcn decorin ( MGI:94872)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006312_01	euxassay_006312_02	euxassay_006312_03	euxassay_006312_04	euxassay_006312_05

euxassay_006312_06	euxassay_006312_07	euxassay_006312_08	euxassay_006312_09	euxassay_006312_10

euxassay_006312_11	euxassay_006312_12	euxassay_006312_13	euxassay_006312_14	euxassay_006312_15

euxassay_006312_16	euxassay_006312_17	euxassay_006312_18	euxassay_006312_19	euxassay_006312_20

euxassay_006312_21	euxassay_006312_22	euxassay_006312_23	euxassay_006312_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 15 16 17 18 19

trigeminal v nerve maxillary division

moderate

regional

moderate expression: see section 05 06 weak expression: see section 07 19 20 21

spinal cord meninges

strong

regional

strong expression: see section 09 12 moderate expression: see section 10 11 13 14

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain meninges

strong

regional

strong expression: see section 09 10 11 14 15 16 17 18 moderate expression: see section 07 08 12 13 19 20

stomach

strong

regional

strong expression: see section 02 03 04 05 06 07 08

palatal shelf

moderate

regional

moderate expression: see section 10 11 14 weak expression: see section 13

diencephalon meninges

strong

regional

strong expression: see section 09 10 11 14 15 16 17 18 moderate expression: see section 08 12 13 19

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 09 10 11 14 15 16 17 18 22 23 24 moderate expression: see section 04 05 06 07 08 12 13 19 20 21

tongue muscle

moderate

regional

moderate expression: see section 10 11 13 14

hindbrain meninges

strong

regional

strong expression: see section 09 10 11 14 15 16 17 18 moderate expression: see section 05 06 07 08 12 13 19 20 21

bladder

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35006
Entity Detected:	Dcn, decorin ( MGI:94872)
Sequence:	sense strand is shown >T35006 CTTCCTTCTGGCACAAGTCTCTTGGGCTGGACCATTTGAACAGAGAGGCTTATTTGACTTCATGCTAGAA GATGAGGCTTCTGGCATAATCCCTTATGACCCTGACAATCCCCTGATATCTATGTGCCCCTACCGATGCC AGTGTCATCTTCGAGTGGTGCAGTGTTCTGATCTGGGTTTGGACAAAGTGCCCTGGGATTTTCCACCCGA CACAACCTTGCTAGACCTGCAAAACAACAAAATTACAGAGATCAAAGAAGGGGCCTTCAAGAACCTGAAG GACTTGCATACCTTGATCCTTGTCAACAACAAGATCAGCAAAATCAGTCCAGAGGCATTCAAACCTCTCG TGAAGTTGGAAAGGCTTTACCTGTCTAAGAACCAACTAAAGGAACTGCCTGAAAAAATGCCCAGAACTCT CCAGGAACTTCGTGTCCATGAGAATGAGATCACCAAGCTGCGGAAATCCGACTTCAATGGACTGAACAAT GTGCTTGTCATAGAACTGGGCGGCAACCCACTGAAAAACTCTGGGATTGAAAACGGAGCCTTCCAGGGAC TGAAGAGTCTCTCATACATTCGCATCTCAGACACCAACATAACTGCGATCCCTCAAGGTCTGCCTACTTC TCTCACTGAAGTGCATCTAGATGGCAACAAGATCACCAAGGTTGATGCACCCAGCCTGAAAGGACTGATT AATTTGTCTAAACTGGGATTGAGCTTCAACAGCATCACCGTTATGGAGAATGGCAGTCTGGCCAATGTTC CTCATCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50055. Forward Primer - name:050055_F_cDNA_Dcn, sequence:CTTCCTTCTGGCACAAGTCTCT; Reverse Primer - name:050055_N_SP6_cDNA_Dcn, sequence:AGATGAGGAACATTGGCCAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006312 same experiment
	EMAGE:31559 same embryo
	EMAGE:31586 same embryo
	EMAGE:30711 same embryo
	EMAGE:31621 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source