EMAGE mouse gene expression spatial database

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EMAGE:32142

Hmbs ( MGI:96112)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002165_16	euxassay_002165_17	euxassay_002165_18	euxassay_002165_19	euxassay_002165_20

euxassay_002165_21	euxassay_002165_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6010
Entity Detected:	Hmbs, ( MGI:96112)
Sequence:	sense strand is shown >T6010 CCCACGCGTCCGGCAGGTCCTGCAGCCGGAGTGAAGTGCGGGCTCGGGCCCCATGTGCCTTCAGTCCCGG CCGGCCCAGGTCGTCGGCTTCTGCAGACACCAGGGGACCGCAGCGGCACTGCCGCGCCTGCGCCCTGGGC GGAGTCATGTCCGGTAACGGCGGCGCGGCCACAACCGCGGAAGAAAACGGCTCAAAGATGAGGGTGATTC GAGTGGGCACCCGTAAGAGCCAGCTGGCTCGCATACAGACCGACACTGTGGTGGCGATGCTGAAAGCCTT GTACCCTGGCATACAGTTTGAAATCATTGCTATGTCCACCACGGGAGACAAGATTCTTGATACTGCACTC TCTAAGATTGGAGAGAAGAGCCTGTTTACCAAGGAGCTAGAAAACGCCCTGGAAAAAAACGAAGTGGACC TGGTTGTTCACTCCCTGAAGGATGTGCCTACCATACTACCTCCTGGCTTTACTATTGGAGCCATCTGCAA ACGGGAAAACCCTTGTGATGCTGTTGTCTTTCACCCAAAGTTTATTGGAAAGACCCTGGAAACCTTGCCA GAGAAAAGTGCCGTGGGAACCAGCTCTCTGAGGAGAGTGGCTCAGCTACAGAGAAAGTTCCCCAACCTGG AATTCAAGAGTATTCGGGGAAACCTCAACACCCGCCTTCGGAAGCTGGATGAGCTGCAGGAATTCAGTGC CATTGTCCTGGCTGTGGCTGGCCTACAGCGCATGGGCTGGCAGAACCGGGTGGGCCAGATTTTGCACCCA GAGGAATGCATGTATGCTGTGGGTCAGGGGGCCCTAGCCGTGGAAGTCCGAGCCAAGGACCAGGATATCT TGGACCTAGTGAGTGTGTTGCACGATCCTGAAACTCTGCTTCGCTGCATTGCTGAAAGGGCTTTTCTGAG GCACCTGGAAGGAGGCTGCAGCGTGCCCGTAGCAGTGCATACAGTGATGAAAGATGGGCAACTGTACCTG ACTGGTGGAGTATGGAGTCTAGATGGCTCAGATAGCATGCAAGAGACTATGCAGGCCACCATCCAGGTCC CTGTTCAGCAAGAAGATGGTCCAGAAGATGACCCACAACTGGTTGGAATCACTGCCCGGAACATTCCAAG AGGAGCCCAGCTAGCTGCTGAGAACCTGGGCATCAGCCTGGCCAGCTTGCTGCTCAACAAAGGAGCCAAG AACATCCTGGATGTTGCACGGCAGCTTAATGATGTGCGCTAACTGGTCTGTAGGGCACAGGAACCCTGGC TGCCACTCCAGTGCCTACTTCTGGCTTCCAAGTGCCCTGTGCTCCATCCCTAGGGGTGTGATTATCCCAG GAAATTGAACCACAGGGTTGTTGAGACTTCCACTTTGGAAGATATGCCTCACCTTGGGGCCTCCATATCT GCCTTTCCCTCAGTAGTTGGGGGCTTCATCTCTTTAGAGAAAGTCCATGCCAATCTTTGAATGTAACCAA TACCACTAATAAACCAGTTTAGAATGTGGTTCTTCTGATAGAGTTGGGGAAGATATGAATAAACCCAAAG CCCTTTTAAACTTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487642 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487642 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002165 same experiment
	EMAGE:32186 same embryo
	EMAGE:29885 same embryo
	EMAGE:32135 same embryo
	EMAGE:29886 same embryo
	EMAGE:29889 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source