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EMAGE:32208

Coq10a ( MGI:2684847)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_016532_01 euxassay_016532_02 euxassay_016532_03 euxassay_016532_04 euxassay_016532_05
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euxassay_016532_06 euxassay_016532_07 euxassay_016532_08 euxassay_016532_09 euxassay_016532_10
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euxassay_016532_11 euxassay_016532_12 euxassay_016532_13 euxassay_016532_14 euxassay_016532_15
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euxassay_016532_16 euxassay_016532_17 euxassay_016532_18 euxassay_016532_19 euxassay_016532_20
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euxassay_016532_21 euxassay_016532_22 euxassay_016532_23 euxassay_016532_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 10 15 16
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 17 18 19 20 21 22
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38747
Entity Detected:Coq10a, ( MGI:2684847)
Sequence:sense strand is shown

>T38747
GTGGACTTCTCGATTTCCTTTGAGTTTCGCTCTCTGCTCCACTCCCAGCTGGCGACCATGTTTTTTGATG
AGGTTGTAAAACAGAATGTTGCTGCCTTTGAGCGCCGGGCAGCAACCAAGTTTGGTCCAGAAACAGCCAT
CCCCCGTGAACTCATGTTTCATGAAGTGCACCAAACTTGAGACGAGGGATTGTTCCTGGCCTCTAGCCCT
CCTCACACCCAGTTTTATTTATTGGTTTGGCTCTTGCTCTGAGAGGTCTAATCTCCTCTCAATTCTTCCT
AAACTGGGCTGCATGCTGTCTGAGCACAGGAAAGATAGCAGGAATGGAAGTGAGACAGAAGAAAGTGTCA
GGTACATTCTGAAAGCCTCACACGCCCAAGGCCGTTGCACACCAAATAAAAACCAGGGCTGTCACCGTCT
CTGTGCCTGGTCGGGGGAATACAGTAGTAACTTCCTGACACAGAGATACTGGCTAGCAAGAAGCCTTTAC
CCTGCTAAGCTTCTCAGGAGAAAAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161472. Forward Primer - name:161472_F_cDNA_mCG18666.1, sequence:GTGGACTTCTCGATTTCCTTTG; Reverse Primer - name:161472_N_SP6_cDNA_mCG18666.1, sequence:GCCTTTTCTCCTGAGAAGCTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_016532 same experiment
 EMAGE:31918 same embryo
 EMAGE:31915 same embryo
 EMAGE:32205 same embryo
 EMAGE:32234 same embryo
 EMAGE:32235 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS