EMAGE mouse gene expression spatial database

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EMAGE:6839

Sirt3 sirtuin 3 (silent mating type information regulation 2, homolog) 3 (S. cerevisiae) ( MGI:1927665)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002666. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002666_01	euxassay_002666_02	euxassay_002666_03	euxassay_002666_04

euxassay_002666_05	euxassay_002666_06	euxassay_002666_07	euxassay_002666_08	euxassay_002666_09

euxassay_002666_10	euxassay_002666_11	euxassay_002666_12	euxassay_002666_13	euxassay_002666_14

euxassay_002666_15	euxassay_002666_16	euxassay_002666_17	euxassay_002666_18	euxassay_002666_19

euxassay_002666_20	euxassay_002666_21	euxassay_002666_22	euxassay_002666_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6839_wholemount_strong.wlz

6839_wholemount_moderate.wlz

6839_wholemount_weak.wlz

6839_wholemount_possible.wlz

6839_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6839_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5033
Entity Detected:	Sirt3, sirtuin 3 (silent mating type information regulation 2, homolog) 3 (S. cerevisiae) ( MGI:1927665)
Sequence:	sense strand is shown >T5033 CGCTAAGCGGTCGACTGGCATTGGCCGCGCTCAGACTGTGGGGTCCGGGAGTGTTACAGGTGGGAGAAGG CCCATATCCCTCTGTGTGGGAGCCTCAGGCGGCTTTGGAGGTGGAGGAAGCAGTGAGAAGAAGTTTTCTC TGCAGGATGTAGCTGAGCTGCTTCGGACCAGAGCCTGCAGTAGGGTGGTGGTCATGGTGGGGGCCGGCAT CAGCACACCCAGTGGCATCCCGGACTTCAGATCCCCAGGGAGCGGCCTCTACAGCAACCTTCAGCAGTAT GACATCCCGTACCCTGAAGCCATCTTTGAACTTGGCTTTTTCTTTCACAACCCCAAGCCCTTTTTCATGT TGGCCAAGGAGCTGTACCCTGGGCACTACAGGCCCAATGTCACTCACTACTTCCTGAGGCTCCTCCACGA CAAGGAGCTGCTTCTGCGGCTCTATACACAGAACATCGACGGGCTTGAGAGAGCATCTGGGATCCCTGCC TCAAAGCTGGTTGAAGCCCACGGGACCTTTGTAACAGCTACATGCACGGTCTGTCGAAGGTCCTTCCCAG GGGAAGACATATGGGCTGATGTGATGGCGGACAGGGTGCCCCGCTGCCCTGTCTGTACTGGCGTTGTGAA ACCCGACATTGTGTTCTTTGGGGAGCAGCTGCCTGCAAGGTTCCTACTCCATATGGCTGACTTCGCTTTG GCAGATCTGCTACTCATTCTTGGGACCTCCCTGGAGGTGGAGCCTTTTGCCAGCTTGTCTGAAGCAGTAC AGAAATCAGTGCCCCGACTGCTCATCAATCGAGACTTGGTGGGGCCGTTCGTTCTGAGTCCTCGAAGGAA AGATGTGGTCCAGCTAGGGGATGTAGTTCATGGTGTGGAAAGGCTGGTGGACCTCCTGGGGTGGACACAA GAACTGCTGGATCTTATGCAGCGGGAACGTGGCAAGCTGGATGGACAGGACAGATAAGACTATGGCTTCT TCACCTGGGGAAGTCACACAGCAGATCATCCTATGTCCAGCAAGACTTCATGCCTGAAGACAGCTCCAAC ACGTTTACAAACATGAACCAGACCACAACATGTGGCCTGGACAGTGGTCCTCCGAGGCTGCCTTTGGAAA GGCTGACCAGGGATGTCTACCCTTGGGGCCCCTCCATGTGTGCGCCCTGTCCACCTCATCACTGCTGAAG GTGTAGTGCAGGTGCTGCTTTCTGCAGCGGCCCTTAAGTTATCACGAGGGCAGCACAGCACGCCCGTCGC CAGGCAGGCGATGCACTAGGGCAATCTAGCATGTTGATCGGTAAAGTGGCATCTTTAACTACAACATCAT TTCTTGCATGAAATAAACTTAGTATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5253854 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5253854 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6836 same embryo
	EMAGE:6841 same embryo
	EMAGE:6837 same embryo
	EMAGE:6840 same embryo
	EMAGE:6838 same embryo
	EurExpress:euxassay_002666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source