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EMAGE:6949

Sap30 sin3 associated polypeptide ( MGI:1929129)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949
"Pseudo-wholemount" of euxassay_002386. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002386_01 euxassay_002386_02 euxassay_002386_03 euxassay_002386_04
EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949
euxassay_002386_05 euxassay_002386_06 euxassay_002386_07 euxassay_002386_08 euxassay_002386_09
EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949
euxassay_002386_10 euxassay_002386_11 euxassay_002386_12 euxassay_002386_13 euxassay_002386_14
EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949
euxassay_002386_15 euxassay_002386_16 euxassay_002386_17 euxassay_002386_18 euxassay_002386_19
EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949 EMAGE:6949
euxassay_002386_20 euxassay_002386_21 euxassay_002386_22 euxassay_002386_23 euxassay_002386_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6949Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6949_wholemount_strong.wlz
6949_wholemount_moderate.wlz
6949_wholemount_weak.wlz
6949_wholemount_possible.wlz
6949_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6949_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1965
Entity Detected:Sap30, sin3 associated polypeptide ( MGI:1929129)
Sequence:sense strand is shown

>T1965
TTTTTTTAATAGTAATAACCTTTATTTAAAATATGTGTAATCTAGGAAGCTCAGTTTATGAGTTTCCAAC
TAATTATTAGAATCAAAAATGACAACAACATTAAAAAAAAAAAAAAAGTCAAAGTAAATCCTCGGTAGAA
AAAATACACAAGAACATTTCTACATCTGAAAACCAGTAAACATTCTTTCCAGGCAGACATCTATGGGCTA
GTGTGAACAGCATCTCTCCTAGTGAACACCACTGTCGGCCTTGAGATCCGATTTGTTCTTGTCGTTCCTC
ACTGAGTAGATGAAACATGTTAAGGTGTCTTTTTCGTTCACTGGAATAGACTTAAAGTGGCAACCAACTA
TCTCAACAAGTTGTGCTTTATTTAGACCTGGTCTGGTTGGAAGCTTGAAGTGTCTTTTGTATCTTCTAAG
TGTATTTACTTGTAATTGGTACAAATCAACCTCTGGAGTGTCGATGTCCTGAACAGGCGAGTCTCCCCCA
TCATCATCGCTTCCTTTCCTCTTCCTTCTGTTCCGAACGCTCTGAATT
Notes:The probe template was PCR amplified from IMAGE:641512 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:641512 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6952 same embryo
 EMAGE:6951 same embryo
 EMAGE:6950 same embryo
 EMAGE:6948 same embryo
 EMAGE:6946 same embryo
 EMAGE:6947 same embryo
 EurExpress:euxassay_002386 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS