EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6973

Blnk B-cell linker ( MGI:96878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002437. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002437_01	euxassay_002437_02	euxassay_002437_03	euxassay_002437_04

euxassay_002437_05	euxassay_002437_06	euxassay_002437_07	euxassay_002437_08	euxassay_002437_09

euxassay_002437_10	euxassay_002437_11	euxassay_002437_12	euxassay_002437_13	euxassay_002437_14

euxassay_002437_15	euxassay_002437_16	euxassay_002437_17	euxassay_002437_18	euxassay_002437_19

euxassay_002437_20	euxassay_002437_21	euxassay_002437_22	euxassay_002437_23	euxassay_002437_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6973_wholemount_strong.wlz

6973_wholemount_moderate.wlz

6973_wholemount_weak.wlz

6973_wholemount_possible.wlz

6973_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6973_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

weak

regional

weak expression: see section 12 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

hindgut

moderate

regional

moderate expression: see section 15 16 17 weak expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 15 16 17 weak expression: see section 11 12 13 14 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5120
Entity Detected:	Blnk, B-cell linker ( MGI:96878)
Sequence:	sense strand is shown >T5120 AGTGGCTTGAGTTCTTGAGGCCAGAGCCTTACCATGCTGCTCCCCAGGAAGTCCAGGAGCTGCTGACACC CCCCTGGACAGCGACACATCCTCTCTCAAGAAAATGGACAAGCTGAATAAGATAACTGTCCCTGCCAGCC AGAAGCTGAGACAGCTTCAAAAGATGGTCCATGATATTAAGAACAATGAAGGTGGAATAATGGACAAGAT AAAAAAGCTAAAAGTCAAAGGCCCTCCAAGTGTTCCTCGAAGGGACTATGCATTAGACAGCCCTGCAGAT GAAGAGGAGCAGTGGTCAGATGACTTCGACAGTGACTATGAAAATCCAGATGAACATTCGGACTCCGAGA TGTATGTGATGCCTGCCGAGGAGACGGGCGACGATTCCTATGAACCGCCTCCCGCTGAGCAGCAGACACG GGTGGTCCATCCAGCCCTGCCCTTCACGAGGGGCGAGTATGTAGATAATCGATCCAGCCAGCGGCACTCT CCGCCCTTCAGCAAGACACTTCCCAGTAAGCCCAGCTGGCCTTCAGCGAAAGCGAGGCTGGCCTCCACTC TGCCAGCCCCCAACTCTCTACAGAAGCCTCAAGTCCCCCCCAAGCCCAAAGACCTCCTTGAGGATGAGGC TGATTATGTGGTCCCTGTGGAAGATAACGATGAAAACTATATCCATCCCAGAGAAAGCAGCCCGCCGCCT GCTGAGAAGGCTCCCATGGTGAATAGATCAACCAAGCCAAACAGTTCCTCAAAGCACATGTCGCCTCCAG GGACTGTCGCAGGTCGAAACAGTGGGGTCTGGGACTCCAAGTCATCTTTGCCTGCCGCACCATCCCCACT ACCACGGGCTGGGAAGAAGCCAGCTACACCACTTAAGACTACTCCCGTTCCTCCCCTACCGAATGCATCA AATGTTTGTGAAGAAAAGCCTGTTCCTGCTGAGCGCCACCGAGGGTCTAGTCACAGACAAGACACTGTAC AGTCACCAGTGTTTCCTCCCACCCAGAAACCTGTCCATCAAAAGCCTGTACCCTTGCCAAGGTTCCCAGA AGCGGGGAGCCCAGCTGCAGATGGACCGTTCCACAGCTTCCCATTTAATTCGACGTTTGCAGACCAGGAG GCTGAACTGCTCGGTAAGCCCTGGTATGCTGGCGCCTGTGACCGCAAGTCTGCTGAAGAGGCCTTGCACA GATCCAACAAGGATGGATCGTTTCTTATTCGGAAGAGCTCTGGCCATGATTCCAAGCAGCCGTACACCCT AGTTGCGTTCTTTAACAAGCGAGTGTATAATATTCCTGTACGGTTTATTGAAGCAACCAAACAGTATGCT TTGGGAAAGAAGAAAAATGGTGAAGAGTACTTCGGAAGTGTTGTGGAAATCGTCAACAGTCACCAGCACA ACCCCCTGGTTCTTATTGACAGTCAGAATAACACGAAAGATTCCACGAGACTGAAATATGCTGTGAAGGT TTCATAACGATACCACGGTTCCAGACATGTCCTCTGTTTCTTCTTTTGAGAAAACATCATATTCTGGCTA TGACTCCTCAGCAGTAAGAGAGAAAAGATGAATGAAGCCACTGAGGCTTCGTGAATGAATGAATCTACTC CTTCCTAGGGCGTTCACACGAGCTTTTCTATCACCTGACCTGACGAAGTCATAGCTGGGGAGGTTCGGTT ACTATGATACTAATATTGTCCAAATAAATGTATTTAAAAGCAAAAAAAAAAAAAAAGGGCGGCCGCTCTA GA
Notes:	The probe template was PCR amplified from IMAGE:5339723 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5339723 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6972 same embryo
	EMAGE:6969 same embryo
	EMAGE:6970 same embryo
	EMAGE:6971 same embryo
	EMAGE:6974 same embryo
	EurExpress:euxassay_002437 same experiment
	MGI:4823479 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source