EMAGE mouse gene expression spatial database

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EMAGE:7044

Pbx3 pre B-cell leukemia transcription factor 3 ( MGI:97496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002571. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002571_01	euxassay_002571_02	euxassay_002571_03	euxassay_002571_04

euxassay_002571_05	euxassay_002571_06	euxassay_002571_07	euxassay_002571_08	euxassay_002571_09

euxassay_002571_10	euxassay_002571_11	euxassay_002571_12	euxassay_002571_13	euxassay_002571_14

euxassay_002571_15	euxassay_002571_16	euxassay_002571_17	euxassay_002571_18	euxassay_002571_19

euxassay_002571_20	euxassay_002571_21	euxassay_002571_22	euxassay_002571_23	euxassay_002571_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7044_wholemount_strong.wlz

7044_wholemount_moderate.wlz

7044_wholemount_weak.wlz

7044_wholemount_possible.wlz

7044_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7044_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23 24 moderate expression: see section 14 15 17

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 18 19 20 moderate expression: see section 16 17

hindbrain

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 05 06 07 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5177
Entity Detected:	Pbx3, pre B-cell leukemia transcription factor 3 ( MGI:97496)
Sequence:	sense strand is shown >T5177 GCCCGCGCGCGGCGGGATGGACGATCAATCCAGGATGCTGCAGACTCTGGCCGGGGTGAACCTGGCCGGC CACTCGGTCCAGGGGGGTATGGCTCTGCCGCCTCCCCCTCACGGCCACGAAGGGGCGGACGGCGACGGCA GGAAGCAGGACATCGGCGACATCCTCCACCAGATCATGACCATCACCGACCAGAGCTTGGACGAGGCGCA AGCAAAGAAACATGCTCTGAACTGTCACAGAATGAAACCGGCGCTCTTCAGCGTCTTGTGTGAGATCAAA GAGAAAACAGGTCTCAGCATCAGAGGAGCCCAGGAGGAAGACCCTCCCGACCCCCAGCTAATGAGACTGG ACAATATGCTTTTGGCAGAAGGGGTTTCAGGTCCTGAGAAAGGTGGGGGATCGGCGGCAGCAGCTGCAGC CGCGGCAGCCTCTGGAGGTTCTTCAGATAACTCTATTGAACACTCAGATTACAGAGCCAAATTGACCCAG ATCAGACAAATCTATCACACAGAACTGGAGAAATATGAACAGGCATGTAATGAATTCACAACACATGTGA TGAACCTTCTCCGAGAACAGAGTAGAACGCGTCCCATTTCCCCGAAAGAGATCGAAAGGATGGTGGGCAT CATCCATCGGAAATTTAGTTCCATTCAGATGCAGCTCAAGCAAAGCACGTGTGAAGCAGTTATGATTTTG AGATCAAGGTTCCTTGATGCCAGACGGAAAAGGCGTAACTTCAGTAAACAGGCTACGGAAATCTTGAATG AATATTTTTACTCACACCTCAGCAACCCCTACCCCAGTGAAGAAGCCAAAGAGGAGCTGGCCAAGAAATG CAGCATCACAGTATCACAGGTATCCAACTGGTTTGGCAACAAACGAATCAGGTACAAGAAGAACATCGGC AAATTTCAAGAGGAGGCCAATCTCTATGCTGCAAAAACAGCCGTGACAGCCGCACATGCAGTGGCTGCAG CCGTGCAGAACAACCAGACCAACTCACCCACCACGCCAAACTCTGGTTCTTCTGGTTCTTTTAACCTCCC AAATTCTGGGGACATGTTCATGAACATGCAGAGTCTGAATGGGGATTCTTACCAAGGGTCCCAAGTCGGA GCCAATGTGCAGTCACAGGTGGATACCCTCCGTCATGTTATCAATCAGACGGGAGGCTACAGTGACGGCC TTGGAGCAAACTCACTGTACAGTCCACATAACTTAAACGCTAATGGAGGCTGGCAGGACGCAACAACTCC ATCTTCTGTGACTTCTCCTACGGAAGGCCCGGGCAGTGTGCACTCGGATACCTCTAACTAATCCCCGGCG CTTTCCCAGCTGACATGCCTTGATAAGCGCATTTGGAGCACTAGGAGAAGGAAAGCGTTTGTAACCCACC ACCTACAGCTTTACTGTAACCCTGTCTTCTTAGAGAACTCGGGAAACCTGTTTTTTAAGGAATCATAATC ATTTGTATTTAAACTTAAGACACACGTTTAAAAAGAAAGCACTTTATCCAATTAGGCCAAGATGTAACAT TGTTGACACTTCTTTAACTGTTTCATCCTAATTTATTATGGGTCTTTTACATTAATGGTTCCACAGTTGC CAGTTCCTTGGCCTTAAGGGTAAGAAGTACCATCTATGCTAGACCCCAATTATAGAGCAGGCAAGGTTCA CCCTGCCTAAGCTGAACCCTTGCGGATTTCCATGCAGGCTCTCATCATCGTTCTCATATCACGGATGGGC TTGGAGGGAAGGCGATGTCTGCCTGTCTTCTTAAGGAAGGCATTTCAGGACTTCCCTTGCCATGTTTGTG TTGCCATCTCTTATGTTGTAATTAGGTCAAAGACTGGTGGCCTCCTTTCTCCCTCTGAAGTACCAGAGCC TGGAGTCTGCGCTAATGAGACCATGGATCCAGATTGCCCTAAGATGAAGATCTTGCTGCTACAGAGGTGA AAACAAGACCTGTCTGGGGTTTGACCGTGTGCGCTAGGTGCTGCACAGACTTGTCAAGGTGTGCTCTTCC TCTGGGCAGCCTGGAAAGGCCCTGCTCTCTGGAGTGCTGTATATAGTGTAGCAAAAGAGTATTTATACAT CCCACCAATCAAAACACAGCTTTATTACCTCATGCGAACTCATACAAACCAATAGAATCCCAACATGTTC TGTAGCTTAGAGCGCTCACTTACTACCTCTGAACAATACTCACGCTGTAGTTTGTCTCTTTCTTATCTTT TTTGCATCTTGTAATTAACTCTTTGTTTCCCTTCACAAAATGTAATGTACATTGTAATCTTTTAAAAGAA AACTCAGGGTTGCATTTGCAACCCTTAAAAAGCCAAGATTGGCAACATTGGGTCTGACTCTTAACACAGG ATCATCCAAGCTGTTGTAAATACTGAGAAGCGTTTTGAATGTTCTTCATCTTGTTACTAATCCAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5348692 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5348692 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7043 same embryo
	EMAGE:7045 same embryo
	EMAGE:7042 same embryo
	EMAGE:7047 same embryo
	EMAGE:7046 same embryo
	EurExpress:euxassay_002571 same experiment
	MGI:4827070 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source