EMAGE mouse gene expression spatial database

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EMAGE:7083

Syt2 synaptotagmin II ( MGI:99666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002635. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002635_01	euxassay_002635_02	euxassay_002635_03	euxassay_002635_04

euxassay_002635_05	euxassay_002635_06	euxassay_002635_07	euxassay_002635_08	euxassay_002635_09

euxassay_002635_10	euxassay_002635_11	euxassay_002635_12	euxassay_002635_13	euxassay_002635_14

euxassay_002635_15	euxassay_002635_16	euxassay_002635_17	euxassay_002635_18	euxassay_002635_19

euxassay_002635_20	euxassay_002635_21	euxassay_002635_22	euxassay_002635_23	euxassay_002635_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7083_wholemount_strong.wlz

7083_wholemount_moderate.wlz

7083_wholemount_weak.wlz

7083_wholemount_possible.wlz

7083_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7083_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex marginal layer

strong

regional

strong expression: see section 09 10 11 12 15 16 17

medulla oblongata basal plate

strong

regional

strong expression: see section 09 10 11 15 16 17

metencephalon basal plate

strong

regional

strong expression: see section 08 09 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 20

dorsal root ganglion

strong

regional

strong expression: see section 09 11 14 15 16 18 19 20 moderate expression: see section 10 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5287
Entity Detected:	Syt2, synaptotagmin II ( MGI:99666)
Sequence:	sense strand is shown >T5287 GCGGGAGGAGAGCCGCAGCGCTGGCCGGCGCGGGAGTGGGAAGAGGGGACCTGGACTTCGGGGCGGCAGA CTCCCGCTGCCTTCTGCACCAGCCCACGCCGAATCGGCTCCCGGACCGTCCCCGGCTATCCCCTCTGCCA CCATGAGAAACATCTTCAAGAGGAACCAGGAGCCAAATGTGGCTCCGGCCACCACCACTGCCACAATGCC CCTTGCACCCGTCGCACCTGCCGACAACTCTACAGAGAGCACGGGTCCTGGGGAGAGCCAAGAAGACATG TTCGCCAAGCTGAAGGAGAAATTCTTCAATGAGATCAACAAGATCCCCTTGCCCCCCTGGGCTCTGATCG CCATGGCTGTGGTTGCTGGCCTCCTGCTGCTCACCTGTTGCTTCTGCATCTGTAAGAAGTGCTGCTGCAA GAAGAAGAAGAACAAGAAGGAGAAGGGCAAAGGCATGAAGAACGCCATGAACATGAAGGACATGAAAGGG GGCCAGGATGACGATGATGCAGAGACAGGCCTGACTGAAGGAGAAGGTGAAGGCGAGGAGGAGAAAGAGC CAGAGAACCTGGGCAAATTGCAGTTTTCTCTGGACTATGATTTCCAGGCCAACCAGCTCACCGTGGGTGT CCTGCAGGCTGCGGAACTCCCAGCCCTGGACATGGGTGGCACATCAGACCCTTATGTCAAAGTCTTCCTC CTCCCAGACAAGAAGAAGAAATATGAGACTAAGGTGCATCGGAAGACGCTGAACCCAGCCTTCAATGAGA CATTCACTTTCAAGGTGCCATACCAGGAGTTAGGAGGCAAGACCCTGGTGATGGCAATCTATGACTTTGA CCGCTTCTCTAAGCATGACATCATCGGGGAGGTGAAGGTACCCATGAACACAGTGGACCTTGGCCAGCCC ATCGAGGAATGGAGAGACCTACAAGGCGGAGAGAAGGAAGAGCCAGAGAAGTTGGGTGACATCTGTACCT CCTTGCGCTACGTGCCCACAGCTGGGAAGCTCACCGTCTGTATCCTGGAGGCCAAGAACCTGAAGAAGAT GGATGTAGGGGGCCTTTCAGACCCCTATGTGAAGATCCACCTGATGCAGAACGGTAAGAGACTCAAGAAG AAGAAGACGACAGTGAAGAAGAAGACCCTGAACCCCTACTTCAACGAGTCCTTCAGCTTCGAGATCCCCT TTGAGCAGATCCAGAAAGTCCAGGTGGTCGTCACCGTGCTAGACTACGACAAACTGGGCAAGAATGAAGC CATCGGAAAGATCTTTGTAGGCAGCAACGCCACAGGCACCGAGTTGCGGCACTGGTCCGACATGCTGGCC AACCCTCGGAGGCCCATTGCCCAGTGGCACTCTCTTAAGCCTGAGGAAGAAGTGGATGCTCTTCTGGGCA AGAACAAGTAGGCTCCAGCGGCCGGGGCCACGCCCCTAAGGAGCCACGCCCCCGAGGCGCCACGCCCCCT GAGGACACTGACGAGATCCAGAGCTATCAATACCTCAGTTACGCGACCTTAGAGGTTTCTTCATTTGTTT GCGGTGTGTCCTGTTTTTCCTTCCTTTTTCTCTTTTTAAAGACCAACTTCCTTTTGGTGGCTGTGTGAAG AGAGTCCCCTAAGAGGTGAAAGAAAAGCCTGGCTCTGTTATTGACCCCGGAGCGGCCCTTGTTGCATGCC CTTTCACGGTTTCCCCCTTACCCCAAGTGGGGCCCTCTACTGTCAGACAGTTGAAGCACTAACTGCTTTT CCTGGGTTTTGGACCAACAACATGGCAAGCACATTCTGTTTCTTGACTGTGAAGGCAACATAGTGGCCAG CATTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACACCTGTATGTGCCCATCCAT CCCCATCTGCCTGTTTTGAACATCTCTCTTCATTTTCTGGAATGAGTCATGGACAGTGAAGCCATGTGAG AGGAGAATGTCTTCAGAGACTCCAAGGGAAAGCAAGCCCACTGCCTAAGCGGTCAGATGCCAGAAGAGAG CTTGCGGATGGCCATTTGGAAGGCGGCGAGATGTGATACTGGATGGGTTCAGAACTGGGACCAAAGCCCT TGATGCCATCATAGACACCCTCTTGTCAGTCATGGCTTTCCCCCCTGGAGTGGGGTTTTGGGGACATTCG TGGAAATCAACTATTCACCAGATTTGCCAGACTCTACCAGGGACTGCCAGCCCTGGGATGTCACTCCTGT TGACAACATCAAACTTTGAAGAAACAGAAGTCCCATTACTCAGAAAGCCTAGAATTAAGCTCCTGGATCT TTGCCTCGTCCCTCGTCCCTGCAGAGTACAAGCAGCGCGGTCATTTGCATTCACGCACCATCTTGCTTTT GCCTCTGTTTCCCTTTTTGTGTAAGTGGAAAAATACCATCTGAAGACAAGTGCTTTGCACAGAGCCAGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362352 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362352 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7086 same embryo
	EMAGE:7087 same embryo
	EMAGE:7085 same embryo
	EMAGE:7084 same embryo
	EurExpress:euxassay_002635 same experiment
	MGI:4828571 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source